نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی کارشناسی ارشد، گروه تربیت بدنی و علوم ورزشی، واحد لامرد، دانشگاه آزاد اسلامی، لامرد، فارس، ایران.
2 استادیار گروه تربیت بدنی و علوم ورزشی، واحد لامرد، دانشگاه آزاد اسلامی، لامرد، فارس، ایران.
3 استاد گروه تربیت بدنی و علوم ورزشی، دانشکده ادبیات و علوم انسانی، دانشگاه لرستان، خرم‌آباد، ایران.
چکیده
زمینه و هدف: تمرینات ورزشی می‌‌تواند به حفظ سلامت و شکل‌‌پذیری دستگاه عصبی کمک کند. تروپومودولین-۲ (Tmod2) نقش‌‌های مهمی در سیستم عصبی مرکزی داشته و با اعمال مختلفی همچون تشکیل سیناپس جدید و افزایش جوانه‌‌زنی عصبی، همراه است. هدف از انجام این مطالعه، بررسی تأثیر تمرین استقامتی با شدت پایین بر سطوح پروتئین Tmod2 و مالون دی آلدئید (MDA) در بافت هیپوکمپ موشهای صحرائی نر ویستار بود. روش تحقیق: پژوهش حاضر تجربی است. برای این منظور تعداد 20 سر موش صحرایی به‌طور تصادفی به دو گروه شامل تمرین (10 موش) و کنترل (10 موش) تقسیم شدند. برنامه تمرین استقامتی به مدت شش هفته و با شدتی برابر با 20 تا 40 درصد حداکثر سرعت دویدن اجرا شد. زمان 48 ساعت پس از آخرین جلسه تمرینی، موش‌‌ها تشریح و بافت هیپوکمپ استخراج شد. جهت سنجش بیان Tmod2 از روش ایمونوهیستوشیمی و برای ارزیابی غلظت MDA از روش الایزا استفاده گردید. برای مقایسه گروه‌ها از آزمون آماری t مستقل در سطح معنی داری 0/05>p استفاده شد. یافته‌‌ها: تمرین استقامتی، بیان Tmod2 را در گروه تمرین نسبت به کنترل به صورت معنی‌‌داری افزایش (0/01=p) و غلظت MDA را به صورت معنی‌‌داری کاهش داد (0/001=p). نتیجه‌‌گیری: تمرین استقامتی اثر سودمندی بر Tmod2 و سیستم عصبی دارد. با این حال، کاهش غلظت MDA می تواند نشانه شاخص استرس اکسایشی باشد؛ تغییری که خود نشان از اثرات محافظتی این شیوه تمرینی در برابر استرس اکسایشی دارد.
کلیدواژه‌ها
عنوان مقاله [English]
The effect of six weeks low-intensity endurance training on tropomodulin-2 protein expression and malondialdehyde in hippocampal tissue of male Wistar rats
نویسندگان [English]
- Seyed Abdolreza Sajjadi 1
- Hadi Ghaedi 2
- Masoud Rahmati 3
1 MS.c Student, Department of Physical Education and Sports Sciences, Lamerd Branch, Islamic Azad University, Lamerd, Fars, Iran.
2 Assistant Professor at Department of Physical Education and Sports Sciences, Lamerd Branch, Islamic Azad University, Lamerd, Fars, Iran.
3 Professor at Department of Physical Education and Sport Sciences, Faculty of Literature and Humanities, Lorestan University, Khorramabad, Iran.
چکیده [English]
Background and Aim: Exercise and training can maintain the health and plasticity of the nervous system. Tropomodulin-2 (Tmod2) plays an important roles in the central nervous system and it correlated with various functions such as the formation of new synapses and increased neuritis extension. The aim of this study was to evaluate the effect of low-intensity endurance training on Tmod2 protein levels and Malondialdehyde (MDA) in the hippocampal tissue of male Wistar rats. Materials and Methods: For this experimental research, 20 rats were randomly divided into two groups including training (n=10) and control (n=10), that for the training group, the endurance training program was run for six weeks at 20-40 percent of maximum running speed. Forty eight hours after the last training session, the rats were dissected and hippocampal tissue was extracted. Immunohistochemistry and Elisa methods were used for measuring the expression of Tmod2 protein and MDA respectively. Independent t-test was used to compare the groups at the significant level of p<0/05. Results: Based on the results, low-intensity endurance training significantly increased the expression of Tmod2 protein (p=0.01) but the MDA concentration (p=0.001) reduced during training as compared to the control group. Conclusion: Endurance training showed a beneficial effect on Tmod2 and also the nervous system; however, reducing the concentration of MDA can be considered as a decreasing of the oxidative stress; a change that shows the protective effects of this training method against oxidative stress.
کلیدواژه‌ها [English]
- Endurance training
- Tropomodulin-2
- Malondialdehyde
- Hippocampus
- Rats
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