نوع مقاله : مقاله پژوهشی
نویسندگان
1 کارشناس ارشد فیزیولوژی ورزشی، دانشگاه آزاد اسلامی، واحد گناباد، ایران
2 استادیار گروه علوم ورزشی، دانشگاه بجنورد، بجنورد، ایران
3 استادیار گروه بیوشیمی بالینی، دانشگاه علوم پزشکی گناباد، گناباد، ایران
چکیده
زمینه و هدف: مطالعات نتایج متناقضی را پیرامون اثر حاد تمرین ورزشی بر شاخصهای التهابی، آسیب اکسایشی و آسیب عضلانی مطرح ساختهاند. از این رو، هدف مطالعه حاضر بررسی اثر حاد فعالیت مقاومتی شدید بر سطوح سرمی عامل نکروز دهنده تومور آلفا (TNF-α) به عنوان شاخص التهابی، مالون دی آلدهید (MDA) به عنوان شاخص فشار اکسایشی، و لاکتات دهیدروژناز (LDH) و کراتین کیناز (CK) به عنوان دو شاخص آسیب عضلانی بود. روش تحقیق: بدین منظور 20 زن سالم غیرفعال (میانگین سن 1/56±21/55 سال و شاخص توده بدنی 3/26±21/68 کیلوگرم بر متر مربع) به طور تصادفی به دو گروه فعالیت مقاومتی شدید و کنترل تقسیم شدند. آزمودنیها فعالیت مقاومتی حاد را با شدت 85 درصد یک تکرار بیشینه در 8 ایستگاه (پرس سینه، پرس پا، قایقی نشسته، پرس بالای سر، باز کردن زانو، باز کردن بازو، خم کردن بازو و بلند کردن پاشنه) در 3 نوبت انجام دادند. قبل و بلافاصله بعد از فعالیت مقاومتی، نمونههای خونی جمع آوری شدند. با استفاده از کیتهای تجاری متغیرهای MDA و TNF-α به روش الایزا اندازهگیری شدند و سطوح LDH و CK به روش فتومتریک مورد سنجش قرار گرفتند. از آزمون tوابسته و مستقل به ترتیب برای مقایسه درون گروهی و بین گروهی متغیرهای وابسته در سطح (0/05>p) استفاده شد. یافتهها: یک جلسه فعالیت مقاومتی شدید موجب افزایش معنیدار (0/001=p) سطوح سرمی TNF-α و MDA شد. همچنین، سطوح سرمی شاخصهای آسیب عضلانی LDH (p=0/03) و CK(p=0/001) متعاقب فعالیت مقاومتی حاد به طور معنیداری افزایش یافت. نتیجه گیری: یک جلسه فعالیت مقاومتی شدید با آسیب اکسایشی و عضلانی در زنان غیرفعال همراه است و اتخاذ تدابیر تمرینی و تغذیهای هنگام اجرای این نوع تمرینات، ضروری به نظر میرسد.
کلیدواژهها
عنوان مقاله [English]
The effect of acute resistance exercise on serum levels of some inflammatory and muscle damage markers in inactive women
نویسندگان [English]
- Mahboobe Ajam Zibad 1
- Hossein TaheriChadorneshin 2
- Seyed Hosein Abtahi Eivary 3
1 MSc in Exercise Physiology, Islamic Azad University, Gonabad Branch, Iran
2 Assistant Professor of Sport Sciences Department, University of Bojnord, Bojnord, Iran
3 Assistant Professor, Clinical Biochemistry Department, Gonabad University of Medical Sciences, Gonabad, Iran
چکیده [English]
Background and Aim: Studies have reported conflicting results regarding the acute effects of exercise on inflammatory, oxidative stress and muscle damage parameters. Therefore, this study aimed to investigate the effect of acute resistance exercise on serum levels of tumor necrosis factor alpha (TNF-α), as a marker of inflammation, malondialdehyde (MDA), as a marker of oxidative stress, and levels of lactate dehydrogenase (LDH) and creatine kinase (CK), as two markers of muscle damage. Materials and Methods: For this purpose, 20 healthy and inactive women (age, 21.55+1.56 year; BMI, 21.68+3.26 kg/m2) were randomly divided into acute resistance exercise and control groups. The subjects performed one bout of acute resistance exercise with 85% of one-repetition maximum (1RM) at 8 stations (bench press, leg press, seated rows, overhead press, seated leg press, arm extension, arm curls and calf raises), each exercise were done for 3 sets. Blood samples were taken before and immediately after exercise. TNF-α and MDA levels were measured using ELISA method by commercial kits. Moreover, LDH and CK were measured by photometric method. Paired sample t-test and independent t-tests were used to analyzed dependent variable within and between groups, respectively (p<0.05). Results: One bout of acute resistance exercise had significant increases (p=0.001) in serum levels of TNF-α and MDA. Also, serum levels of muscle damage parameters, LDH (p=0.03) and CK (p=0.001), showed significantly increases following acute resistance exercise. Conclusion: One bout of intense resistance exercise is associated with oxidative and muscle damage in inactive women, therefore adoption of training and nutritional approaches are essential when implementing with this kind of exercise.
کلیدواژهها [English]
- Acute resistance exercise
- Tumor necrosis factor alpha
- Malondialdehyde
- Lactate Dehydrogenase
- Creatine Kinase
supplementation on malondialdehyde and total thiol in non-athlete women. Zahedan Journal of Research in Medical Sciences, 15, 29-33.
Aguilo, A., Tauler, P., & Fuentespina, E. (2005). Antioxidant response to oxidative stress induced by exhaustive
exercise. Physiology & Behavior, 84(1), 1-7.
Ahmadi, A., Agha Alinejad, H., & Gharakhanlo, R., Zarifi, A. (2009). Study of relationship between serum alterations of
interleukin-6 and inactive girls after tow kind of concentric and eccentric submaximal exersice. Olympic, 46, 63-72.
[Persian]
Baghaee, B., Tartibian, B., & Baradaran, B. (2013). The effect of gender differences on relationship between total antioxidant status and inflammatory enzyme fallowing to intensive aerobic exercise in young athletes individual. Journal of Sabzevar University of Medical Science, 4(66), 345-353. [Persian]
Barquilha, G., Uchida, M.C., Santos, V.C., Moura, N.R., Lambertucci, R.H., Hatanaka, E., Cury-Boaventura, M.F.,
Pithon-Curi, T.C., Gorjão, R., & Hirabara, S.M. (2011). Characterization of the effects of one maximal repetition
test on muscle injury and inflammation markers. Physiology, 2(3), WMC001717.
Bloomer, R.J., Goldfarb, A.H., Wideman, L., McKenzie, M.J., & Consitt, L.A. (2005). Effects of acute aerobic and anaerobic
exercise on blood markers of oxidative stress. Journal of Strength & Conditioning Research, 19(2), 276-85.
Bloomer, R.J. & Smith, W.A. (2011). Oxidative stress in response to aerobic and anaerobic power testing: influence of
exercise training and carnitine supplemention. Research in Sport Medicine, 17, 1-16.
Brandauer, J., Landers-Ramos R.Q., Jenkins, N.T., Spangenburg, E.E., Hagberg, J.M., & Prior S.J. (2013). Effects of prior acute exercise on circulating cytokine concentration responses to a high-fat meal. Physiological Reports, 1(3), 1-10.
Buckley, J.D., Thomson, R.L., Coates, A.M., Howe, P.R., DeNichilo, M.O., & Rowney, M.K. (2010). Supplementation with
a whey protein hydrolysate enhances recovery of muscle force-generating capacity following eccentric exercise. Journal of
Science and Medicine in Sport, 13(1), 178-81.
indices of oxidative stress. Journal of Strength & Conditioning Research, 24(9), 2491-2497.
Calle, M.C., & Fernandez, M.L. (2010). Effects of resistance training on the inflammatory response. Nutrition Research
and Practice, 4(4), 259–269.
Changizi, M., Ebrahimi, M., & Avandi, M. (2015). Acute effects of coenzyme Q10 supplement on serum parameters of
oxidative stress following one session of resistance training in male college athletes. Koomesh, 16(4), 603-610.
[Persian]
Cooper, C.E., Vollaard, N.B.J., Choueiri T., & Wilson. M.T. (2002). Exercise, free radicals and oxidative stress.
Biochemical Society Transactions, 30(2), 280 – 285.
Deminice, R., Rosa, F.T., Franco, G.S., Jordao, A.A., & de Freitas, E.C. (2013). Effects of creatine supplementation on
oxidative stress and inflammatory markers after repeated-sprint exercise in humans. Nutrition, 29(9),1127-32.
Dixon, C.B., Robertson, R.J., Goss, F.L., Timmer, J.M., Nagle, E.F., & Evans, R.W. (2006). The effect of acute
resistance exercise on serum malondialdehyde in resistance-trained and untrained collegiate men. Journal of
Strength & Conditioning Research, 20(3), 693-8.
Fatouros, I., Chatzinikolaou, A., Paltoglou, G., Petridou, A., Avloniti, A., Jamurtas, A., Goussetis, E., Mitrakou, A.,
Mougios V., Lazaropoulou, C., Margeli, A., Papassotiriou, I., & Mastorakos, G. (2010). Acute resistance exercise results in
catecholaminergic rather than hypothalamic-pituitary-adrenal axis stimulation during exercise in young men.
Stress, 13(6), 461-8.
Felger, J.C., & Lotrich, F.E. (2013). Inflammatory cytokines in depression: neurobiological mechanisms and therapeutic
implications. Neuroscience, 246, 199-229.
Gadruni, K., Mahmmadpour, H., & Gadruni, M. (2015). Effect of elastic-band exercise on muscle damage
and inflammatory responses in Taekwondo athletes. Revista Brasileira de Medicina do Esporte, 21(4), 297-301.
Ghasemi, E., Afzalpour, M.E., Saghebjoo, M., & Zarban, A. (2012). Effects of short-term green tea supplementation on
total antioxidant capacity and lipid peroxidation in young women after a resistance training session. Journal of Isfahan
Medical School, 30(202), 1267-1268. [Persian]
Gonzalez, A.M., Hoffman, J.R., Jajtner, A.R., Townsend, J.R., Boone, C.H., Beyer, K.S., Baker, K.M., Wells, A.J.,
Church, D.D., Mangine, G.T., Oliveira, L.P., Moon, J.R., Fukuda, D.H., & Stout, J.R. (2015). Protein supplementation
does not alter intramuscular anabolic signaling or endocrine response after resistance exercise in trained men. Nutrition
Research, 35(11), 990-1000.
Gorzi, A., Agha Alinejad, H., Rajabi, H., & Azad, A. (2012). Effect of concurrent, strength and endurance training on hormones, lipids and inflammatory characteristics of untrained men. Iranian Journal of Endocrinology and Metabolism, 13(6), 614-620. [Persian]
Hammouda, O., Chtourou, H., Chaouachi, A., Chahed, H., Ferchichi, S., Kallel, C., Chamari, K., & Souissi N. (2012). Effect of short-term maximal exercise on biochemical markers of muscle damage, total antioxidant status, and homocysteine levels in football players. Asian Journal of Sports Medicine, 3(4), 239-46.
Kirwan, J.P., Krishnan, R.K., Weaver, J.A., Del Aguila, L.F., & Evans, W.J. (2001). Human aging is associated
with altered TNF-alpha production during hyperglycemia and hyperinsulinemia. American Journal of Physiology
Endocrinology and Metabolism, 281(6), 1137-43.
Musculoskeletal and Neuronal Interactions, 14(1), 68-77.
Miles, M.P., & Stephen, B. (2004). IL-6, CRP, and CK responses to a 20-mile race at altitude in women verses men.
Medicine & Science in Sports & Exercise, 36(5), S150.
Nameni, F., Kashef, M., & Lari, A.A. (2004). Effect of warm up on relationship between CK and LDH in recovery period
in athletic women. Olympic, 12(4), 97-107. [Persian]
Nayebifar, S., Afzalpour, M.E., Saghebjoo, M., Hedayati, M., & Shirzaee, P. (2012). The effect of aerobic and resistance
trainings on serum C- reactive protein, lipid profile and body composition in overweight women. Modern Care Journal, 8
(4), 186-196. [Persian]
Nikolaidis, M.G., Kyparos, K., Hadziioannou, M., Panou, N., Samaras, L., Jamurtas, A.Z., & Kouretas, D. (2007). Acute
exercise markedly increases blood oxidative stress in boys and girls. Applied Physiology Nutrition and Metabolism, 32,
197–205.
Pareja-Blanco, F., Rodríguez-Rosell, D., Sánchez-Medina, L., Ribas-Serna, J., López-López, C., Mora-Custodio, R.,
Yáñez-García J.M., & González-Badillo, J.J. (2016). Acute and delayed response to resistance exercise leading or not
leading to muscle failure. Clinical Physiology and Functional Imaging, 36(5), 1-10.
Penkowa, M., Keller, C., Keller, P., Jauffred, S., & Pedersen, B.K. (2003). Immunohistochemical detection of interleukin-6 in human skeletal muscle fibers following exercise. Federation of American Societies for Experimental Biology Journal, 17, 2166-2168.
Powers, S.K., & Jackson, M.J. (2008). Exercise-induced oxidative stress: cellular mechanisms and impact on muscle
force production. Physiological Reviews, 88(4),1243-76.
Rahman, Z.A., Abdullah, N., Singh, R., & Sosroseno, W. (2010). Effect of acute exercise on the levels of salivary cortisol,
tumor necrosis factor-alpha and nitric oxide. Journal of Oral Science, 52(1), 133-6.
Rogers, M.A., Stull, G.A., & Apple, F.S. (1985). Creatine kinase isoenzyme activities in men and women following a
marathon race. Medicine & Science in Sports & Exercise, 17(6), 679-82.
Rosene, J., Matthews, T., Ryan, C., Belmore, K., Bergsten, A., Blaisdell, J., Gaylord, J., Love, R., Marrone, M., Ward, K.,
& Wilson, E. (2009). Short and longer-term effects of creatine supplementation on exercise induced muscle damage.
Journal of Sports Science and Medicine, 8(1), 89-96.
Saghebjoo, M., Ghanbari-Niaki, A., Rajabi, H., Rahbarizadeh, F., & Hedayati, M. (2011). The influence of circuit resistance
training intensity on ghrelin to obestatin ratio of plasma in healthy young women. Iranian Journal of Endocrinology
and Metabolism, 12(6), 626-632. [Persian]
Smart, N.A., Larsen, AI, Le Maitre, J.P., & Ferraz, A.S. (2011). Effect of exercise training on interleukin-6, tumour necrosis factor alpha and functional capacity in heart failure. Cardiology Research and Practice, 2011, 532620.
Townsend, J.R., Hoffman, J.R., Fragala, M.S., Jajtner, A.R., Gonzalez, A.M., Wells, A.J., Mangine, G.T., Fukuda, D.H., &
Stout, J.R. (2015). TNF-α and TNFR1 responses to recovery therapies following acute resistance exercise. Frontiers in
Physiology, 6, 1-6.
Ullum, H., Martin, M., Diamant, M., Boer, A.G., & Breimer, D.D. (1985). Bicycle exercise enhances plasma IL-6 but does
not change IL-1 alpha, IL-1 beta, IL-6, or TNF-alpha pre-mRNA in BMNC. Journal of Applied Physiology, 77(1), 93-97.
effects of enhanced eccentric and concentric resistance exercise on metabolism and inflammation. Journal of Novel
Physiotherapies, 4(2),1-17.
Yamashita, N., Hoshida, S., Otsu, K., Asahi, M., Kuzuya, T., & Hori, M. (1999). Exercise provides direct biphasic cardioprotection via manganese superoxide dismu-tase activation. Journal of Experimental Medicine, 189(11),
1699–706.
Zarghami Khameneh, Z., Jafari, A., & Akhtari Shojaei, E. (2014). The effect of acute caffeine ingestion on oxidative
response in male volleyball players following one-session resistance exhaustive exercise. Sport Physiology, 6
(22), 115-130. [Persian]