نوع مقاله : مقاله پژوهشی
نویسندگان
1 کارشناس ارشد فیزیولوژی ورزش/ دانشکده تربیت بدنی/ دانشگاه اصفهان/ اصفهان
2 دانشیار فیزیولوژی ورزش/ دانشکده تربیت بدنی/ دانشگاه اصفهان/ اصفهان
3 استاد بیوشیمی بالینی/ مرکز تحقیقات علوم دارویی دانشکده داروسازی وعلوم دارویی/ دانشگاه علوم پزشکی اصفهان
4 کارشناس ارشد بیوتکنولوژی/ شرکت تحقیقاتی حکیمان شرق اصفهان/ اصفهان
5 دانشجوی دکتری فیزیولوژی ورزش/ دانشکده تربیت بدنی/ دانشگاه اصفهان/ اصفهان
چکیده
زمینه و هدف : هدف از تحقیق حاضر بررسی تغییرات ظرفیت آنتی اکسیدانی تام و پراکسید هیدروژن و در نتیجه استرس اکسیداتیو احتمالی ناشی از فعالیت ورزشی حاد، در موش های نر نژاد ویستار می باشد. روش تحقیق: 14 سر موش صحرایی نر به صورت تصادفی به دو گروه کنترل و فعالیت ورزشی حاد تقسیم شدند. گروه فعالیت ورزشی حاد با شدت تمرینی 16-26 متر در دقیقه برای یک ساعت، تا رسیدن به واماندگی بر روی نوارگردان دویدند. خونگیری یک ساعت پس از فعالیت ورزشی حاد صورت گرفت. برای اندازه گیری سطح سرمی ظرفیت آنتی اکسیدانی تام و پراکسید هیدروژن به ترتیب از روش FRAP و FOX- 1 استفاده شد. جهت تعیین طبیعی بودن توزیع داده ها از آزمون کولموگروف اسمیرنوف استفاده شد و سپس نتایج با استفاده از آزمون t مستقل در سطح p<0/05 استخراج گردید. یافته ها: طبق یافته های به دست آمده، ظرفیت آنتی اکسیدانی تام (0/002=p) و پراکسید هیدروژن (0/003=p) درگروه فعالیت ورزشی حاد در مقایسه با گروه کنترل، به طور معنی دار افزایش یافت. نتیجه گیری: هر چند در پاسخ به فعالیت ورزشی حاد، میزان پراکسید هیدروژن افزایش یافت، اما بالا رفتن ظرفیت آنتی اکسیدانی موش ها می تواند دال بر واکنش و پاسخ سیستم دفاعی آنتی اکسیدانی بدن به ورزش حاد اجرا شده باشد.
کلیدواژه‌ها
عنوان مقاله [English]
The effect of acute exercise on total antioxidant capacity and hydrogen peroxide in male Wistar rats
نویسندگان [English]
- Masume Kazemi 1
- Sayed Mohammad Marandi 2
- Ahmad Movahedian Attar 3
- Mona Haghighatian 4
- Zeinab Rezaee 5
1
2
3
4
5
چکیده [English]
Background and Aim: The aim of this study was to determine the amount of total antioxidant capacity and hydrogen peroxide changes after an acute exercise in Wistar rats. Material and Methods: 14 male Wistar rats were randomly divided into two groups as control and acute exercise groups. Acute exercise intensity was set to 16 to 26 m/min for one hour, or until exhaustion on a treadmill running. Blood samples were collected 1 hour after exercise. Total antioxidant capacity and hydrogen peroxide were detected by FRAP and FOX-1 methods respectively. When the normal distribution of the data was revealed by Kolmogorov-Smirnov test, it is applied independent t-test at significance level of pResults: Results showed that TAC (p= 0.002) and H2O2 (p= 0.003) significantly increased in the acute exercise group as compared with control group. Conclusion: Although the level of hydrogen peroxide increased in response to the acute exercise, improved antioxidant capacity may be indicator of response of rats antioxidant system to the performed acute exercise.
کلیدواژه‌ها [English]
- Acute exercise
- Oxidative stress
- Hydrogen peroxide
- Antioxidant capacity
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