نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی دوره دکتری فیزیولوژی ورزشی، گروه علوم ورزشی، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران
2 دانشیار فیزیولوژی ورزشی، گروه علوم ورزشی، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران
3 دانشیار گروه زیست‌شناسی، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران.
4 استادیار فیزیولوژی ورزشی، گروه علوم ورزشی، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران
چکیده
زمینه و هدف: عدم تنظیم در میکرو آر ان اِ ها (miRNAs)، نقش کلیدی در بروز اختلالات رایجی از جمله درد، در بیماری پارکینسون دارند. هدف مطالعه حاضر، بررسی اثر یک دوره تمرین شنا بر miRNAs مرتبط با درد، در موش‌های مبتلا به پارکینسون بود. روش تحقیق: تعداد 21 سرموش نر نژاد ویستار هشت تا 10 هفته‌ای، در سه گروه (هر گروه هفت سر موش) شامل گروه سالم، بیمار، و تمرین شنا تقسیم شدند. به گروه بیمار و تمرین، با تزریق یک میلی‌گرم به ازای هر کیلو وزن رزرپین، بیماری پارکینسون القا شد. تمرین شنای تناوبی شدید به مدت شش هفته در قالب 20 نوبت 30 ثانیه‌ای، با 30 ثانیه استراحت بین نوبت‌ها؛ با تکرار سه جلسه در هفته، اجرا گردید. بیان ژن های mir-23b و mir-let-7 در بافت هیپوکامپ، با روش Real Time-PCR اندازه‌گیری شد. تغییرات بیان ژن با استفاده از آزمون تحلیل واریانس یک راهه و آزمون تعقیبی LSD در سطح معنی‌داری 05/0>p تجزیه و تحلیل شدند. یافتهها: بیان ژن هیپوکامپی mir-23b در گروه بیمار نسبت به گروه سالم، به طور معنی‌داری پایین‌تر بود (01/0=p)؛ اما تفاوتی بین گروه بیمار با گروه تمرین (22/0=p) و بین گروه تمرین و گروه سالم (09/0=p)؛ مشاهده نشد. از طرف دیگر، بیان ژن هیپوکامپی mir-let-7 تفاوت معنی‌داری بین گروه بیمار و گروه سالم نداشت (50/0=p)؛ و تفاوت معنی‌داری در بیان این ژن، بین گروه تمرین و سالم (82/0=p)، و بین گروه بیمار و تمرین (64/0=p)؛ مشاهده نشد. نتیجهگیری: به نظر میرسد تمرینات شنای تناوبی شدید تأثیر چندانی بر miRNAs مرتبط با درد، در موش‌های پارکینسونی ندارد؛ هرچند به دلیل محدودیت های موجود، نیاز به بررسی های بیشتر می باشد.
کلیدواژه‌ها
عنوان مقاله [English]
The effect of swimming training on hypocampic mir-23b and mir-let-7 gene expression related to pain in Wistar rats with Parkinson’s disease
نویسندگان [English]
- Reza Zeinolebadi 1
- Mehrzad Moghadasi 2
- Mohammdamin Edalatmanesh 3
- Mehdi Noora 4
1 PhD student in exercise physiology, Department of exercise physiology, Shiraz branch, Islamic Azad University, Shiraz, Iran
2 Associate professor of exercise physiology, Department of exercise physiology, Shiraz branch, Islamic Azad University, Shiraz, Iran
3 Associate Professor at Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
4 Assistant professor of exercise physiology, Department of exercise physiology, Shiraz branch, Islamic Azad University, Shiraz, Iran
چکیده [English]
Background and Aim: Dysregulated miRNAs play critical roles in many disorders such as pain in Parkinson’s disease (PD). The aim of this study was to examine the effect of swimming training on hypocampic miRNAs gene expression related to pain in rats with PD. Materials and Methods: Twenty-one male Wistar rats (age eight to 10 weeks) were divided (n=7) into healthy control, PD, and training groups. PD was induced by injection of one mg/kg Reserpine to rats in PD and training groups. The rats in the training group performed six weeks of high-intensity interval training, including 20 times of 30 seconds of swimming with 30 seconds rest between each time and three times a week. mir-23b and mir-let-7 gene expressions were measured in hippocampus using Real Time-PCR method. Data were analyzed using one-way ANOVA and LSD tests at a significant level of p<0.05. Results: The hypocampic mir-23b gene expression was significantly lower in PD compare to the healthy control group (p=0.01), while no significant difference was observed between PD and training groups (p=0.22); and between healthy control and training groups (p=0.09). On the other hand, hypocampic mir-let-7 gene expression did not significantly difference between PD and healthy control group (p=0.50); moreover, no significant difference was observed between PD and training (p=0.82); and healthy and training (p=0.64) groups. Conclusion: It seems that high-intensity interval swimming training did not affect miRNAs related pain in rats with PD; however, due to existing limitations, more investigations are needed.
کلیدواژه‌ها [English]
- Parkinson’s disease
- Swimming training
- Pain
- miRNAs gene expression
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