نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی دکترای فیزیولوژی ورزشی، گروه تربیت بدنی و علوم ورزشی، دانشگاه آزاد اسلامی، بجنورد، ایران
2 استادیار فیزیولوژی ورزشی، گروه تربیت بدنی و علوم ورزشی، دانشگاه آزاد اسلامی، بجنورد، ایران
3 استاد فیزیولوژی، گروه فیزیولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران
چکیده
زمینه و هدف: بیماری‌های التهابی روده دسته‌ای از بیماری‌های مزمن دستگاه گوارش می‌باشند که با اسهال، درد شکم و خونریزی در مدفوع مشخص می شوند و درمان آن ها هنوز با چالش هایی مواجه است. هدف مطالعه حاضر بررسی اثر تمرینات هوازی شنا به تنهایی و همراه با آب حاوی هیدروژن بر علایم، تغییرات بافت شناسی و شاخص های استرس اکسیداتیو در بیماری التهابی روده در مدل تجربی می باشد. روش تحقیق: تعداد 30 سر موش نر نژاد C57BL/6 به طور تصادفی به گروه های کنترل، تمرین شنا، کولیت، کولیت+ تمرین شنا، کولیت+ تمرین شنا+ آب حاوی هیدروژن (ترکیبی) تقسیم شدند. در پایان آزمایش، وزن بدن، طول کولون، تغییرات بافت شناسی و شاخص های استرس اکسایشی اندازه گیری شدند. تجزیه و تحلیل آماری با استفاده از آزمون آماری تحلیل واریانس یک راهه و دو راهه در سطح معنی داری 0/05˂p صورت گرفت. یافته ها: در حیوانات مبتلا به کولیت، شاخصهای شدت فعالیت بیماری (کاهش وزن، خونریزی و بیرون‌زدگی رکتوم و قوام مدفوع) در گروه تمرین شنا در مقایسه با گروه کولیت، به طور معنی‌دار کاهش یافت (0/05˂p)؛ به گونه ای که این شاخص در گروه درمان ترکیبی در مقایسه با گروه تمرین تنها، کاهش بیشتری داشت (0/001˂p). طول کولون در گروه‌های تمرین شنا و گروه ترکیبی در مقایسه با گروه کولیت، به طور معنی دار افزایش یافت (0/05˂p). به علاوه، تغییرات بافت‌شناسی در گروه‌های ترکیبی در مقایسه با گروه کولیت، به‌طور معنی‌داری بهبود یافت (0/01˂p). حیوانات مبتلا به کولیت دارای سطح بافتی بالای شاخص مالون دی آلدئید و کاهش شاخصهای آنتی‌اکسیدانی (گروه های تام تیول، سوپراکسید دسموتاز و کاتالاز) نسبت به گروه کنترل بودند (001/0>p) و تمرینات شنا بخصوص در ترکیب با آب حاوی هیدروژن، به طور معنی داری سبب افزایش عوامل آنتی اکسیدان و کاهش مالون دی آلدئید گردید (0/05˂p). نتیجه گیری: تمرینات هوازی شنا بخصوص در ترکیب با آب حاوی هیدروژن، سبب بهبود علائم و تغییرات هیستولوژیک در کولیت می‌شود و ازاین‌رو، می‌تواند در کنار سایر داروهای معمول، به درمان کولیت کمک نماید.
کلیدواژه‌ها
عنوان مقاله [English]
The effect of aerobic exercise swimming in combination with hydrogen rich water on clinical symptoms, histological changes and oxidative stress markers in experimental colitis model
نویسندگان [English]
- Zohreh Kolahi 1
- Ali Yaghoubi 2
- Najmeh Rezaeian 2
- Majid Khazaei 3
1 Ph.D student in exercise physiology, Department of Sport Science, Islamic Azad University, Bojnourd, Iran.
2 Assistant Professor of exercise physiology, Department of Sport Science, Islamic Azad University, Bojnourd, Iran.
3 Professor of Medical Physiology, Department of Physiology, Mashhad University of Medical Sciences, Mashhad, Iran.
چکیده [English]
Background and Aim: Inflammatory bowel disease (IBD) is a group of chronic gastrointestinal diseases that pose a challenge to its treatment. This study aimed to investigate the effect of aerobic swimming exercise and in combination with hydrogen-rich water (HRW) on clinical symptoms, histological changes and oxidative stress markers in an animal model of colitis. Materials and Methods: Thirty male C57BL6 mice were divided into control, swimming exercise, colitis, colitis+swimming exercise, colitis+swimming exercise+ colitis groups. At the end of study, colon weight and length, spleen weight, disease activity index, histological changes and oxidative stress markers in colon tissue were evaluated. Data were analyzed using one-way or two-way ANOVA and p<0.05 was considered statistically significant. Results: Results showed that there were no significant differences between control and swimming exercised groups. But, in colitis animals, the disease activity index (such as weight loss, bleeding, and rectal protrusion) in exercised group decreased significantly compared to the colitis group; which showed a further decrease in the combined group (p<0.001). Moreover, colon length was also increased in exercised groups compared to the colitis group (p<0.05). Histological indices (such as leukocyte infiltration) and fibrosis were significantly decreased in exercsied groups compared to the colitis group (p<0.01). Colitis animals had higher malondialdehyde and lower antioxidative markers (superoxihde dismutase, catalase, total thiol) compared to the control group; while exercise and exercise plus HRW significantly improved antioxidative factors. Conclusion: The results showed that swimming exercise especially in combination with HRW could improve clinical symptoms and histological changes of colon tissue in colitis animals and it can be considered as treatment strategy in patients with colitis.
کلیدواژه‌ها [English]
- Swimming aerobic exercise
- Inflammatory bowel disease
- Hydrogen rich water
- colitis
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