نوع مقاله : مقاله پژوهشی
نویسندگان
1 کارشناسی ارشد فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه پیام نور، البرز، ایران.
2 استادیار گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه پیام نور، تهران، ایران.
چکیده
زمینه و هدف: فرآیند توسعه عروق جدید، شامل تحریک رشد، مهاجرت و تثبیت عروق جدید می‌شود که به شدت تحت تاثیر عوامل مختلف تحریک‌کننده و مهارکننده، مانند ترومبوسپوندین-2 (TSP-2) و عامل رشد تغییرشکل دهنده - بتا (TGF-β) قرار می‌گیرد. هدف از این مطالعه، مقایسه اثر هشت هفته روش‌های مختلف تمرین هوازی بر بیان ژن‌های آنژیوستاتیک TSP-2 و TGF-β بافت چربی زیرجلدی موش‌های صحرایی بود. روش تحقیق: در این پژوهش تجربی، ۳۲ سر موش صحرایی نر ویستار با میانگین وزن 237 گرم به صورت تصادفی به چهار گروه (هشت سر در هر گروه) شامل گروه‌های شم، تمرین با شدت بالا (HIT)، تمرین با شدت متوسط (MIT) و تمرین تناوبی با شدت بالا (HIIT) تقسیم شدند. پروتکل‌های تمرین شامل دویدن روی نوارگردان به مدت هشت هفته با شدت‌ها و مدت های مختلف بود. اندازه‌گیری بیان ژن‌ها با روش RT-PCR صورت گرفت. داده‌های حاصل با آزمون های تحلیل واریانس یک‌راهه و توکی، در سطح معنی داری 05/0≥p تحلیل شدند. یافته‌ها: هر سه شیوه تمرینی، بیان TGF-β را در مقایسه با گروه شم، به طور معنی داری کاهش داد؛ اما تفاوت اثر معنی داری بین شیوه‌های تمرینی مشاهده نشد. با این حال، تنها پروتکل‌های HIT و MIT باعث کاهش معنی دار بیان TSP-2 در مقایسه با گروه شم شدند و HIT در مقایسه با پروتکل HIIT، کاهش بیشتری در بیان TSP-2 ایجاد کرد. نتیجه‌گیری: احتمالا تمرینات هوازی، به ویژه HIT با شدت و مدت مشخص، از طریق تاثیر بر بیان ژن‌های آنژیوستاتیک در بهبود خون‌رسانی به بافت چربی و متعاقبا کاهش توده چربی، موثر هستند.
کلیدواژه‌ها
عنوان مقاله [English]
Comparison of the effect of eight weeks of different aerobic training methods on the expression of angiostatic genes TSP-2 and TGF-β in the subcutaneous adipose tissue of rats
نویسندگان [English]
- Zohreh Yaghoubi 1
- Saeed Naghibi 2
- Maryam Vatandoust 2
1 MSc of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Payame Noor University, Alborz, Iran.
2 Assistant Professor at Exercise Physiology Department, Faculty of Physical Education and Sport Sciences, Payame Noor University, Tehran, Iran.
چکیده [English]
Background and Aim: The development of new blood vessels involves the stimulation of growth, migration, and stabilization, which is influenced by various stimulating and inhibiting factors such as Thrombospondin-2 (TSP-2) and transforming growth factor-beta (TGF-β). This study aimed to compare the effect of eight weeks of different aerobic training methods on the expression of TSP-2 and TGF-β genes in subcutaneous adipose tissue of rats. Materials and Methods: The study conducted an experimental research on 32 male Wistar rats to investigate the effects of different training protocols on gene expression. The rats were divided into four groups (n=8): scheme, high-intensity training (HIT), moderate-intensity training (MIT), and high-intensity interval training (HIIT). The training protocols involved treadmill running for eight weeks, with varying intensities and durations for each group. Gene expression was measured by RT-PCR method. The data were analyzed with one-way ANOVA and Tukey’s tests at a significance level of p≤0.05. Results: The results of the study show that all three training methods led to a significant reduction in TGF-β expression compared to the scheme group. However, no significant difference was observed between training methods. It was also found that only the HIT and MIT methods caused a significant decrease in TSP-2 expression compared to the scheme group, and that HIT could significantly reduce the expression of TSP-2 compared to the HIIT protocol. Conclusion: In conclusion, it is suggested that aerobic exercises, particularly HIT with specific intensity and duration, may be effective in improving blood supply to fat tissue and subsequently reducing fat mass through their impact on the expression of angiostatic genes.
کلیدواژه‌ها [English]
- Exercise training
- Transforming growth factor-beta
- Thrombospondin-2
- Angiogenesis
- Adipose tissue
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