نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی دکتری فیزیولوژی پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکی تبریز، تبریز، ایران.
2 استادیار گروه فیزیولوژی، دانشکده دامپزشکی، علوم پزشکی تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.
چکیده
زمینه و هدف: آواریکتومی (برداشت تخمدان) باعث القاء استرس اکسیداتیو در بدن می‌شود. دانه کتان به عنوان منبع غنی از آنتی‌اکسیدان‌های طبیعی همراه با فعالیت بدنی منظم، می‌تواند اثرات مخرب ناشی از استرس اکسیداتیو را مهار کند. هدف تحقیق حاضر بررسی اثر ترکیبی عصاره کتان و تمرین هوازی بر وضعیت اکسیداتیو بافت قلب در موش‌های صحرایی آواریکتومی شده بود. روش تحقیق: در این مطالعه تجربی، از 36 سر موش صحرایی ماده بالغ نژاد ویستار، با میانگین وزن 25±250 گرم استفاده شد. موش‌ها به‌طور تصادفی به شش گروه مساوی (n=6) شامل گروه های کنترل، شم (شاهد جراحی)، آواریکتومی شده، آواریکتومی- عصاره بذر کتان، آواریکتومی- تمرین، و نهایتا آواریکتومی- تمرین- عصاره بذر کتان؛ تقسیم شدند. تمرینات هوازی به مدت 8 هفته، هر هفته 5 روز بر روی نوارگردان به اجرا درآمد و گروه های تیمار با عصاره بذر کتان، روزانه به مقدار 400 میلی‌گرم/کیلوگرم وزن بدن عصاره بذر کتان به‌صورت گاواژ دریافت کردند. در پایان دوره مطالعه، نمونه بافت قلب از حیوانات مختلف اخذ گردید و ظرفیت تام آنتی اکسیدانی (TAC)، غلظت مالون دی آلدهید (MDA)، به همراه فعالیت آنزیم های سوپر اکساید دیسمیوتاز (SOD)، گلوتاتیون پراکسیداز (GPx) و پارااکسوناز-1 (PON1) اندازه‌گیری گردیدند. داده های جمع آوری شده با آزمون های آماری تحلیل واریانس یک‌طرفه و تعقیبی توکی در سطح 0/05>p مورد بررسی قرار گرفتند. یافته‌ها: فعالیت آنزیم‌های SOD، GPX، PON1 و میزان TAC در موش‌های گروه آواریکتومی به‌طور معنی‌داری کمتر از موش‌های گروه کنترل بود؛ در حالی که MDA به طور معنی داری افزایش پیدا کرد (05/p<0). از طرف دیگر، فعالیت آنزیم‌های SOD، GPX، PON1 و میزان TAC در موش‌های صحرایی گروه آواریکتومی- تمرین - عصاره به‌طور معنی‌داری در مقایسه با گروه‌های آواریکتومی، آواریکتومی- عصاره و آواریکتومی- تمرین افزایش پیدا کرد؛ در حالی که MDA به طور معنی داری کاهش یافت (05/p<0). نتیجه‌گیری: ترکیب عصاره بذر کتان و تمرین هوازی تاثیر بهتری در بهبود فعالیت سیستم آنتی‌اکسیدانی و کاهش شاخص اکسیداسیون بافت قلب در موش صحرایی آواریکتومی شده دارد.
کلیدواژه‌ها
عنوان مقاله [English]
The effect of flaxseed alcoholic extract and aerobic exercise on oxidative status of heart tissue in ovariectomized rats
نویسندگان [English]
- Seyed Zanyar Athari 1
- Miralireza Nourazar 2
- Mohammadreza Nasirzadeh 2
1 PhD Student in Medical Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Assistant Professor, Department of Physiology, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran.
چکیده [English]
Background and Aim: Ovariectomy can induces oxidative stress in the body. Flaxseed is known as a rich source of natural antioxidants along with regular physical activity can also inhibit the destructive effect of oxidative stress. The present study aimed to evaluate the combined effect of flaxseed extract and aerobic exercise on the oxidative status of cardiac tissue in ovariectomized rats. Materials and Methods: In this experimental study, 36 adult female Wistar rats weighing 250±25 gr were randomly divided into six equal groups (n=6) including healthy control, sham (surgical control), ovariectomy, ovariectomy-flaxseed extract, ovariectomy-exercise, and ovariectomy-exercise-flaxseed extract group. Aerobic exercises five days a week for eight weeks were performed on a treadmill. Moreover, the flaxseed treatment groups received 400 mg/kg body weight of flaxseed extract by gavage. At the end of the study, cardiac tissue samples from different animals were obtained and then total antioxidant capacity (TAC), malondialdehyde concentration (MDA), the activity of glutathione peroxidase (GPX), superoxide dismutase (SOD), and paraoxonase 1 (PON1) enzymes were measured. The data were analyzed by one-way analysis of variance (ANOVA) followed by post hoc Tukey test at the significant level of p<0.05. Results: The results showed that the activity of SOD, GPX, PON1 enzymes and TAC in ovariectomy mice was significantly lower than control mice but the MDA index was significantly increased (p<0.05). However the activity of SOD, GPX, PON1 enzymes and TAC levels were significantly higher and the MDA concentration was significantly lower (p<0.05) in mice of ovariectomy-exercise-extract group than the mice of ovariectomy, ovariectomy-extract and ovariectomy-exercise groups. Conclusion: The results of this study showed that the combination of flaxseed extract and aerobic exercise more improved the activity of the antioxidant system, reduced the oxidation index of heart tissue in menopause and also the stress caused by aging.
کلیدواژه‌ها [English]
- Aerobic-exercise
- Flaxseed-Extract
- Heart
- Oxidative-stress
- Rat
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