نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی، گروه تربیت بدنی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران

2 استادیار، گروه تربیت بدنی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران

3 دانشیار، گروه فیزیولوژی ورزشی، دانشگاه شهید مدنی آذربایجان، تبریز، ایران

4 دانشیار فیزیولوژی ورزشی، گروه تربیت بدنی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران

5 استادیار، گروه دامپزشکی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران

چکیده

زمینه و هدف: مواجهه با آرسنیک با احتمال وقوع آپوپتوزیس در کبد و نارسایی‌های متابولیک همراه است؛ در حالی که تمرین تناوبی شدید (HIIT) و کورکومین می‌توانند این شرایط را به طور مثبت تعدیل نمایند. هدف تحقیق حاضر، بررسی تاثیر HIIT و کورکومین بر عامل دو وابسته به عامل هسته‎ای اریتروئید-2 (NRF2) و کاسپاز-3 کبدی، و ظرفیت ضداکسایشی تام (TAC)، گلوکز، تری‌گلیسرید (TG) و لیپوپروتئین کلسترول پرچگال (HDL) خون در موش‌های در معرض آرسنیک بود. روش‌ تحقیق: در این تحقیق تجربی، 48 موش نر به شش گروه شامل آرسنیک-HIIT (تمرین)، آرسنیک-کورکومین (کورکومین)، آرسنیک-کورکومین-تمرین (توام)، آرسنیک، کنترل اتانول، و کنترل معمولی تقسیم‌‌ شدند. آرسنیک و کورکومین به ترتیب با دوز 5 و 15 میلی‌گرم بر هر کیلوگرم وزن بدن در روز از طریق گاواژ به موش‌ها خورانده شدند. پروتکل HIIT به مدت شش هفته، پنج روز در هفته و هر جلسه شامل 60 دقیقه فعالیت (تناوب‌های چهار دقیقه‌ای دویدن با شدت 90-85 درصد vVO2max، با دو دقیقه استراحت فعال) اجرا شد. داده‌ها با روش‌های رنگ‌سنجی و وسترن بلات استخراج شدند و با روش تحلیل واریانس یک راهه در سطح 05/0>p مقایسه گردیدند. یافته‌ها: در گروه آرسنیک، میزان کاسپاز-3 کبد، گلوکز و TGخون بالاتر؛ و TAC و HDL خون پایین‌تر از گروه کنترل معمولی بود (001/0=p در همه موارد)؛ در حالی که HDL، گلوکز و  TAC خون در سه گروه تمرین، کورکومین و توام؛ و کاسپاز -3 کبد تنها در گروه توام؛ تفاوت معنی‌داری با گروه کنترل معمولی نداشتند (05/0<p). به علاوه، NRF2 کبدی فقط در گروه‌های ‌کورکومین و توام به بالاتر از سطح گروه کنترل رسید (001/0=p). نتیجه‌گیری: با این که تاثیر آرسنیک بر افزایش TG خون، با مداخله تمرین، کورکومین و یا اثر توام آن‌ها، اصلاح نشد؛ ولی هر سه مداخله افزایش قند و کاهش HDL و TAC خون را اصلاح کردند. از طرف دیگر، افزایش کاسپاز-3 کبدی فقط با مداخله توام؛ و کاهش NRF2 کبدی فقط با کورکومین اصلاح شد. 

کلیدواژه‌ها

عنوان مقاله [English]

Effects of high intensity interval training and curcumin on blood total antioxidant capacity and hepatic NRF2 and caspase-3 level in rats exposed to arsenic

نویسندگان [English]

  • Nooshin Salehi aghdam 1
  • Rogayeh pouzesh jadidi 2
  • Karim Azali Alamdari 3
  • Jabbar Bashiri 4
  • Mir Ali Reza Nourazar 5

1 : PHD student of Exercise Physiology, Department of Physical Education, Tabriz branch, Islamic Azad University, Tabriz, Iran

2 Assistant Professor, Department of Exercise Physiology, Tabriz branch, Islamic Azad University, Tabriz, Iran

3 Associate Professor of Exercise Physiology, Azarbaijan Shahid Madani University, Tabriz, Iran

4 Department of Physical Education, Tabriz branch, Islamic Azad University, Pasdaran Highway, Tabriz, Iran

5 Department of Vet, Tabriz branch, Islamic Azad University, Pasdaran Highway, Tabriz, Iran

چکیده [English]

Background and Aim: Arsenic exposure could likely lead to hepatic apoptosis and metabolic disturbances and high intensity interval training (HIIT) as well as curcumin supplementation seems to improve this condition. The aim of the present study was to investigate the effects of HIIT and curcumin supplementation on hepatic nuclear factor erythroid 2-related factor 2 (NRF2) and caspase-3 as well as blood total antioxidant capacity (TAC), glucose, triglyceride (TG), and high density lipoprotein cholesterol (HDL) in rats exposed to arsenic. Materials and Methods: During the experimental study, 48 male rats were randomized into six groups of arsenic-HIIT (HIIT), arsenic-curcumin (curcumin), arsenic-HIIT-curcumin (concomitant), arsenic, ethanol control, and normal control. Arsenic and curcumin (5 and 15 mg/bw/day respectively) were consumed by gavage method. HIIT performed six weeks, five d/w, 60 min/session, consisted of running bouts (four min) at 85-90% of vVo2max with two min active rest intervals. The data were measured using colorimetry and Wester blotting and were analyzed by one-way ANOVA at the p<0.05. Results: Hepatic caspase-3 as well as blood glucose and TG were significantly higher, and blood TAC and HDL levels were lower in arsenic group compared to normal control (p=0.001 under any circumstances). However, blood HDL, glucose and TAC in all three groups of HIIT, curcumin and concomitant as well as liver caspase-3 just in concomitant group had not significant difference as compared to control group (p>0.05). Additionally, hepatic NRF2 were elevated to levels even higher than control group in curcumin and concomitantc groups (p=0.001). Conclusion: Although the up-regulated blood TG-induced by arsenic could not restore with HIIT, curcumin or concomitant interventions, however, three interventions efficiently restore the elevated blood glucose and also the lowered HDL and TAC. Moreover, increased hepatic caspase-3 was only corrected with concomitant intervention, while only curcumin could restore the lowered levels of hepatic NRF2 induced by arsenic. 

کلیدواژه‌ها [English]

  • Apoptosis
  • Arsenic
  • High intensity interval training
  • Curcumin
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