نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشیار فیزیولوژی ورزشی ، گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تبریز
2 دانشیار، گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تبریز، تبریز، ایران.
3 دانشجوی دکترای فیزیولوژی ورزشی دانشکده تربیت بدنی و علوم ورزشی دانشگاه تبریز
چکیده
زمینه و هدف: میکرو RNA (miRNA)، RNA های کوتاهی هستند که به عنوان تنظیم‌گرهای مهم فرآیندهای فیزیولوژیک مرتبط با چاقی، نقش مهمی در بدن بر عهده دارند؛ این در حالی است که ترکیب تأثیر تمرین تناوبی و تداومی همراه با محدودیت کالریک، می تواند بیان ژن miR-146a و پاره ای از عوامل التهابی همچون عامل تومور نکروزی آلفا (TNF-α)، اینترلوکین-6 (IL-6) و پروتئین واکنشی C (CRP) را تحت تاثیر قرار دهد. از این رو، مطالعه حاضر با هدف مقایسه دو نوع تمرین ورزشی به همراه محدودیت کالریک متناوب (ICR) بر بیان ژن miR-146a و سطوح سرمی TNF-α، IL-6وCRP در زنان چاق به اجرا درآمد. روش تحقیق: از میان زنان 45- 35 ساله چاق شهر تبریز، 28 نفر به صورت نمونه گیری هدف‌دار انتخاب و به طور تصادفی در چهار گروه ICR (ICR؛20 درصد)، ترکیب تمرین تناوبی شدید و ICR (10 درصد محدودیت کالری از تمرین و 10 درصد ICR)، ترکیب تمرین تداومی و ICR (10 درصد محدودیت کالری از تمرین و 10 درصد ICR)، و کنترل قرار گرفتند. برنامه تمرین تناوبی (با شدت 95-75 درصد حداکثر ضربان قلب ذخیره) و تداومی (با شدت 75-60 حداکثر ضربان قلب ذخیره) به مدت 8 هفته و با تکرار 3 جلسه در هفته به اجرا درآمد. متغیرهای وابسته تحقیق قبل از مداخله و 72 ساعت پس از آخرین جلسه تمرین اندازه‌گیری شدند. نتایج با استفاده از آزمون‌های شاپیرو- ویلک، تحلیل واریانس با اندازه گیری مکرر و بونفرونی در سطح معنی‌داری 05/0>p استخراج گردید. یافته‌ها: هر دو گروه تمرینی همراه با محدودیت کالریک در مقایسه با گروه کنترل، افزایش معنی‌داری در میزان حداکثر اکسیژن مصرفی و سطح بیانmiRNA-146a و کاهش معنی‌دار در TNF-α، IL-6 وCRP را نشان دادند (05/0>(p؛ در حالی که بین دو گروه تمرین تناوبی و تداومی هوازی همراه با محدودیت کالریک، تفاوت معنی‌داری مشاهده نشد (05/0< .(pنتیجه‌گیری: انجام تمرینات ورزشی (صرف نظر از تناوبی بودن یا تداومی بودن آن) و محدود کردن کالری دریافتی روزانه، می‌تواند در کاهش التهاب مزمن افراد چاق موثر بوده و به عنوان یک روش غیردارویی، برای کمک به حل مشکلات این قبیل افراد، مورد استفاده قرار گیرد.
کلیدواژه‌ها
عنوان مقاله [English]
Comparison of changes in miR-146a gene expression and serum levels of TNF-α, IL-6 and CRP following interval or continuous aerobic training with calorie restriction in obese women
نویسندگان [English]
- vahid sari saarraf 1
- Ramin Amirsasan 2
- Seyyedeh Fareideh Iraqi 3
1 1. Associate Professor in Exercise Physiology, Department of Exercise Physiology, School of Physical Education and Sport Sciences, University of Tabriz, Tabriz, Iran.
2 Associate Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tabriz, Iran.
3 3. Ph.D. student, Department of Exercise Physiology, School of Physical Education and Sport Sciences, University of Tabriz, Tabriz, Iran
چکیده [English]
Background and Aim: Micro-RNAs (miRNA) are short RNA molecules that as significant regulators of physiological processes associated with obesity play important roles in body; this is while the combination of effect of interval and continuous aerobic training along with calorie restriction can affect the gene expression of miR-146a and some inflammatory factors like tumor necrosis factor- α (TNF-α), interleukin -6 (IL-6) and C-reactive protein (CRP). Therefore, the aim of the present study was to determine the effect of two types of exercise training along with intermittent calorie restriction (ICR) on miR-146a expression and TNF-α, IL-6 and CRP levels in obese women. Materials and Methods: Twenty-eight women were selected by targeted sampling from obese women aged 35-45 years in Tabriz city and were divided randomly into four groups including of ICR (ICR, 20 percent), high intensity interval training and ICR (10 percent calorie restriction from exercise and 10 percent ICR), a combination of continuous aerobic training and ICR (10 percent calorie restriction from exercise and 10 percent ICR) and control groups. Interval training (up to 75-95% of the maximum reserve heart rate) and continuous training (up to 60-75% of the maximum reserve heart rate) was performed for 8 weeks with repetition of 3 sessions per week. Dependent variables were measured before the intervention and 72 hours after the last training session. Results were extracted using Shapiro-Wilk, repeated measure analysis of variance and Bonferroni tests at the significance level of p<0.05. Findings: Both training groups with calorie restriction compared to the control group showed a significant increase in maximal oxygen uptake and miR-146a expression and a significant decrease in TNF-α, IL-6 and CRP (p<0.05). While, there was no significant difference between the two groups of interval and continuous aerobic exercise with calorie restriction (p<0.05.) Conclusion: Doing exercise (regardless of whether it is interval or continuous) and limiting daily calorie intake can be effective in reducing chronic inflammation in obese people and can be used as a non-pharmacological method to help solve the problems of these people.
کلیدواژه‌ها [English]
- Exercise training
- Calorie intake
- miR-146a gene
- Inflammatory markers
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