نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی، دانشکده علوم‌‌تربیتی و روان‌‌شناسی، دانشگاه محقّق اردبیلی، اردبیل، ایران.

2 دانشیار گروه فیزیولوژی ورزشی، دانشکده علوم‌‌تربیتی و روان‌‌شناسی، دانشگاه محقّق اردبیلی، اردبیل، ایران.

3 دانشیار گروه تربیت بدنی و علوم ورزشی، دانشکده علوم انسانی، دانشگاه کاشان، کاشان، ایران.

چکیده

زمینه و هدف:  نقش محافظتی گلوتامین در برابر تجزیه پروتئین‌‌ها و اثر بالقوه آن در بازتوانی پس از فعالیت‌‌های درمانده‌‌ساز، به خوبی مشخص نشده است. هدف از این مطالعه، بررسی تاثیر مکمل‌‌دهی حاد گلوتامین قبل از یک فعالیت درمانده‌‌ساز بر میزان لاکتات خون و شاخص درد در ورزشکاران جوان بود. روش‌ تحقیق: در این مطالعه نیمه تجربی دوسوکور، 16 ورزشکار مرد (سن: 77/1±87/21 سال، وزن: 02/6±65/72 کیلوگرم، نمایه توده بدن: 75/1± 23 کیلوگرم برمترمربع) به‌‌صورت تصادفی در دو گروه مکمل گلوتامین (8=n) و دارونما (8=n) تقسیم شدند. گروه تجربی، 6/0 گرم مکمل گلوتامین همراه با 500 میلی‌لیتر آب، به ازای هر کیلوگرم از وزن بدن نیم ‌‌ساعت قبل از فعالیت نوشیدند. گروه دارونما محلول دو درصد دکسترین را بدون گلوتامین مصرف نمودند. میزان لاکتات با استفاده از دستگاه لاکتومتر و شاخص درد با پرسشنامه مقیاس عددی درد (NPRS)، در فواصل زمانی قبل، بلافاصله، 30 دقیقه و 60 دقیقه پس از پروتکل درمانده‌‌ساز اندازه‌گیری شد. داده‌‌ها با استفاده از آزمون تحلیل واریانس با اندازه‌گیری مکرر و آزمون تعقیبی بونفرونی در سطح معنی داری 05/0>p مورد بررسی قرار گرفتند. یافته‌ها: سطح لاکتات خون  و شاخص درد  بلافاصله پس از فعالیت درمانده‌‌ساز در هر دو گروه به طور معنی‌داری افزایش یافت (05/0>p).  در زمان 30 دقیقه پس از اتمام فعالیت، سطح لاکتات خون و شاخص درد نسبت به بلافاصله بعد از فعالیت، در هر دو گروه کاهش معنی‌داری داشت (05/0>p). روند کاهش سطح لاکتات و شاخص درد  تا 60 دقیقه پس از اتمام فعالیت همچنان ادامه داشت، اما این کاهش در گروه مکمل گلوتامین بیشتر از گروه دارونما بود (05/0>p). همچنین تفاوت بین‌‌گروهی معنی‌داری در سطح لاکتات خون (30 و60 دقیقه) و شاخص درد (بلافاصله، 30 و60 دقیقه) بعد از فعالیت درمانده‌‌ساز وجود داشت (05/0>p). نتیجه‌گیری: پیشنهاد می‌شود ورزشکاران از مکمل گلوتامین، برای کاهش سطح لاکتات خون و شاخص درد قبل از اجرای فعالیت‌‌های درمانده‌‌ساز استفاده کنند.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of acute glutamine supplementation before an exhaustive activity on blood lactate level and pain index in young athletes

نویسندگان [English]

  • Hamed Ghiami taklimi 1
  • Ameneh Pourrahim Ghouroghchi 2
  • Mohammad Ebrahim Bahram 1
  • Mohammad Javad Pourvaghar 3

1 PhD Student in Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran.

2 Associate Professor, Department of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran.

3 Associate Professor, Department of Physical Education and Sport Sciences, Faculty of Humanities, University of Kashan, Kashan, Iran.

چکیده [English]

Background and Aim: The protective role of glutamine against protein breakdown and also its potential effect on rehabilitation after exhaustive activities is not well understood. The aim of this study was to investigate the effect of acute glutamine supplementation before an exhaustive activity on blood lactate levels and pain index in young athletes. Materials and Methods: In this quasi-experimental double-blind study, 16 male athletes (age: 21.87±1.77 years, weight: 72.65±6.02 kg, body mass index: 23±1.75 kg/m2) were randomly divided into two groups including glutamine (n= 8) and placebo (n= 8) groups. The experimental group consumed 0.6 g of glutamine supplement per kg in body weight with 500 ml of water half an hour before activity. Moreover, the placebo group used 2% dextrin solution without glutamine. Lactate level was measured using a lactometer and pain index was evaluated with the numerical pain raiting scale (NPRS) questionnaire before, immediately, 30 minutes and 60 minutes after an exhaustive protocol. Data were analyzed using repeated measures analysis of variance and Beferroni post hoc test was applied at the significance level of p<0.05. Results: Blood lactate level and pain index significantly increased after exhaustive activity in both groups (p<0.05). However, blood lactate levels and pain index were significantly lower 30 minutes after activity as compare of the initial phase after exercise in both groups (p<0.05). The decreasing process of lactate level and pain index continued up to 60 minutes after the end of activity, but this decrease was greater in the glutamine supplement than in the placebo group (p<0.05). There was also a significant difference between blood lactate levels (30 and 60 minutes) and pain index (immediately, 30 and 60 minutes) after the exhaustive activity (p<0.05). Conclusion: It is recommended that athletes can use glutamine supplementation to reduce blood lactate levels and pain index before their performing exhaustive activities.

کلیدواژه‌ها [English]

  • Pain index
  • Acute physical activity
  • Blood lactate
  • Glutamine
Abbey, J., Piller, N., Bellis, A.D., Esterman, A., Parker, D., Giles, L., Lowcay, B. (2004). The Abbey pain scale a 1-minute numerical indicator for people with end-stage dementia. International Journal of Palliative Nursing, 10(1), 6-13.
Akbarnezhad, A., Ravasi, A.A., Aminian, R.T. and Nourmohammadi, I. (2006). The effect of creatine and glutamine supplements on athletic performance in elite wrestlers after one acute period of weight losing. Harkat, 27, 173-188. [Persian]
Bae, J.Y., Koo, G.H., Park, S.C., & Shin, K.O. (2019). Effects of branched-chain amino acid and glutamine supplementation on angiogenic factors and pro-Inflammatory cytokines after ecute exercise in adolescence athletes. The Asian Journal of Kinesiology, 21(2), 51-58. 
Bassini-Cameron, A., Monteiro, A., Gomes, A., Werneck-de-Castro, J.P.S.M & Cameron, L. (2008). Glutamine protects against increases in blood ammonia in football players in an exercise intensity-dependent way. British Journal of Sports Medicine, 42(4), 260-266.
Birrer, D., & Morgan, G. (2010). Psychological skills training as a way to enhance an athlete’s performance in high‐intensity sports. Scandinavian Journal of Medicine & Science in Sports, 20(1), 78-87.
Brancaccio, P., Lippi, G., & Maffulli, N., (2010). Biochemical markers of muscular damage. Clinical Chemistry and Laboratory Medicine, 48(6), 757-767.
Cruzat, V.F., & Tirapegui, J. (2009). Effects of oral supplementation with glutamine and alanyl-glutamine on glutamine, glutamate, and glutathione status in trained rats and subjected to long-duration exercise. Nutrition, 25(4), 428-435.
Cruzat, V.F., Rogero, M.M., & Tirapegui, J. (2010). Effects of supplementation with free glutamine and the dipeptide alanyl‐glutamine on parameters of muscle damage and inflammation in rats submitted to prolonged exercise. Cell Biochemistry and Function, 28(1), 24-30.
Curi, R., Lagranha, C.J., Doi, S.Q., Sellitti, D.F., Procópio, J., Pithon‐Curi, T.C., Corless, M., & Newsholme, P. (2005). Molecular mechanisms of glutamine action. Journal of Cellular Physiology, 204(2), 392-401. 
Engel, J.M., Pitz, S., Mühling, J., Menges, T., Martens, F., Kwapisz, M., & Hempelmann, G. (2009). Role of glutamine administration on T-cell derived inflammatory response after cardiopulmonary bypass. Clinical Nutrition, 28(1), 15-20.
Faghfouri Azar, M., Bayat, M., Jamali Fashi, R., Vesali, M. (2017). The comparison between body composition, cardio-respiratory fitness, balance, and mental health in active and inactive elderly women. Journal of Health, 8 (4), 464-474. [Persian]
Falk, D.J., HEELAN, K.A., Thyfault, J.P., & Koch, A.J. (2003). Effects of effervescent creatine, ribose, and glutamine supplementation on muscular strength, muscular endurance, and body composition. The Journal of Strength & Conditioning Research, 17(4), 810-816.
Farrokh Shahinia, R., Rahmani Nia, F., Farzaneh, E. (2012). The effect of glutamine supplementation on perceived pain        intensity and changes in creatine kinase enzyme levels following extraverted exercise in untrained men. Exercise Physiology, 5(19), 97-110. [Persian]
Favano, A., Santos-Silva, P.R., Nakano, E.Y., Pedrinelli, A., Hernandez, A.J., & Greve, J.M.D. (2008). Peptide glutamine supplementation for tolerance of intermittent exercise in soccer players. Clinics, 63(1), 27-32.
Gaeini, A.A., Zafari, A. (2004). Comparison of two recovery programs (active and inactive) on the changes in blood lactate due to intensive exhaustive activity. Quarterly Olympics 13(4), 21-30. [Persian]
Gleeson, M. (2008). Dosing and efficacy of glutamine supplementation in human exercise and sport training. The Journal of  Nutrition, 138(10), 2045-2049.
Golmakani, N., Hashemi Asl, B.M., Sajadi, S.A., & Ebrahimzadeh, S. (2012). The relationship between happiness during pregnancy, and labor pain coping behaviors. Evidence Based Care, 2(2), 85-93. [Persian]
Howatson, G., & Van Someren, K.A. (2008). The prevention and treatment of exercise-induced muscle damage. Sports Medicine, 38(6), 483-503.
Kaminsky, L.A., & Whaley, M.H. (1998). Evaluation of a new standardized ramp protocol: the BSU/Bruce Ramp protocol. Journal of Cardiopulmonary Rehabilitation and Prevention, 18(6), 438-444.
Laaksonen, M.S., Kivelä, R., Kyröläinen, H., Sipilä, S., Selänne, H., Lautamäki, R., ... & Komi, P.V. (2006). Effects of exhaustive stretch‐shortening cycle exercise on muscle blood flow during exercise. Acta Physiologica, 186(4), 261-270.
Lee, B., Diaz, G.A., Rhead, W., Lichter-Konecki, U., Feigenbaum, A., Berry, S.A., … & Berquist, W. (2015). Blood ammonia and glutamine as predictors of hyperammonemic crises in patients with urea cycle disorder. Genetics in Medicine, 17(7), 561-568.
Moeini Najafabadi, A., Rahmani-Nia, F., Mirzaei, B., Eslampour, A. (2019). ‘The effect of combined creatine, glutamine, and turin supplementation on the response to muscle and liver damage induced by high intensity interval exercise in trained men. Journal of Practical Studies of Biosciences in Sport, 7(14), 67-79. [Persian]
Mohammadzadeh, S.K., & Abdollahi, S. (2018). The effect of glutamine supplementation and active recovery on blood lactate levels after one-stop exit activity in active men. In Procceding of the International Conference on Sports Sciences, July, Iran,Tehran,1-6. [Persian]
Molfino, A., Logorelli, F., Muscaritoli, M., Cascino, A., Preziosa, I., Fanelli, F.R., & Laviano, A. (2010). Metabolic effects of glutamine on insulin sensitivity. Nutritional Therapy & Metabolism, 28(1) , 7-11.
Montgomery, P.G., & Hopkins, W.G. (2013). The effects of game and training loads on perceptual responses of muscle soreness in Australian football. Internationa Journal of Sports Physiology and Performance, 8(3), 312-318.
Moscatelli, F., Valenzano, A., Petito, A., Triggiani, A.I., Ciliberti, M.A.P., Luongo, L., … & Monda, M. (2016). Relationship between blood lactate and cortical excitability between taekwondo athletes and non-athletes after hand-grip exercise. Somatosensory & Motor Research, 33(2), 137-144.
Najarzadeh, A., Atarod, H., Mozaffari-Khosravi, H., Dehghani, A., & Asjodi, F. (2015). The effect of single portion glutamine supplement consumption on injury indices of muscle after eccentric resistance exercise. Arak Medical University Journal, 18(97), 9-17. [Persian]
Nelson, A.R., Karagounis, L.G., & Rowlands, D.S. (2015). Leucine-Protein Supplemented Recovery and Exercise. 1th Edition. New York, In: Branched Chain Amino Acids in Clinical Nutrition. Humana Press.
Nosaka, K., Newton, M., & Sacco, P. (2002). Delayed‐onset muscle soreness does not reflect the magnitude of eccentric exercise‐induced muscle damage. Scandinavian Journal of Medicine & Science in Sports, 12(6), 337-346.
Nourshahi, M., Kaviani, M., Kimiagar, M., & Ebrahim, K.H. (2009). The effects of acute L-carnitine supplementation on anaerobic threshold and lactate accumulation during an incremental exercise. Iranian Journal of Nutrition Sciences & Food Technology, 4(2), 45-52. [Persian]
Pithon-Curi, T.C., Schumacher, R.I., Freitas, J.J., Lagranha, C., Newsholme, P., Palanch, A.C., … & Curi, R. (2003). Glutamine delays spontaneous apoptosis in neutrophils. American Journal of Physiology-Cell Physiology, 284(6), 1355-1361.
Rahmani Nia, F., Farzaneh, E., Damirchi, A., Shamsi Majlan, A., & Farokhshahi, R. (2014). The effect of glutamine supplementation on delayed onset muscle soreness and electromyographic activity after eccentric contraction in untrained men. Journal of Sport in Biomotor Sciences, 16(6), 31-40. [Persian]
Razzaghi, A., Kashef, M., & Gaeini, A.A. (2017). Effect of short-term glutamine supplementation on Vo2max and capillary blood lactate during recovery after maximum exercise in men athlete. Journal of Applied Exercise Physiology, 13(25), 115-124. [Persian] 
Sadeghi, A., & Husseini, M. (2017). Short-term effects of glutamine supplementation on levels of blood lactate and fatigue index in male elite swimmers. European Online Journal of Natural and Social Sciences, 6(1), 81-87. [Persian]
Tabrizi, A., Ravasi, A., Gaeini, A., & Gholipour, M. (2010). The comparison of the effects of active and passive recovery on immune system indexes after a graded exhaustive exercise in college athletes. Journal of Sport Biosciences, 2(5), 5-17. [Persian]
Vescovi, J.D., Falenchuk, O., & Wells, G.D. (2011). Blood lactate concentration and clearance in elite swimmers during competition. International Journal of Sports Physiology and performance, 6(1), 106-117.  
Zheng, L., Cardaci, S., Jerby, L., MacKenzie, E.D., Sciacovelli, M., Johnson, T.I., ... & Hedley, A. (2015). Fumarate induces redox-dependent senescence by modifying glutathione metabolism. Nature Communications, 6(1), 1-12.