نوع مقاله : مقاله پژوهشی
نویسندگان
1 کارشناس ارشد علوم ورزشی، دانشکده علوم ورزشی، دانشگاه بیرجند، بیرجند، ایران.
2 دانشیار گروه علوم آزمایشگاهی، دانشکده پیراپزشکی، دانشگاه علوم پزشکی گناباد، گناباد، ایران.
3 دانشجوی دکترای فیزیولوژی ورزش، دانشکده علوم ورزشی، دانشگاه بجنورد، بجنورد، ایران.
چکیده
زمینه و هدف: تمرینات ورزشی متوسط تا شدید باعث آسیب بافت‌‌های بدن می‌‌گردد، اما استفاده از مکمل‌‌های آنتی اکسیدانی می‌‌تواند استرس اکسیداتیو ناشی از تمرین ورزشی را تعدیل نماید. هدف تحقیق حاضر بررسی تغییرات سرمی آنزیم گلوتاتیون پراکسیداز (GPX) و سوپراکسید دیسموتاز (SOD) دختران غیرفعال مصرف کننده کوآنزیم Q10 پس از تمرینات حاد مقاومتی متوسط و شدید بود. روش‌‌تحقیق: نوع تحقیق نیمه تجربی می‌‌باشد. تعداد 27 دانشجوی دختر به طور تصادفی در سه گروه (دو گروه تمرین مقاومتی و یک گروه کنترل) قرار گرفتند. گروه‌‌های تجربی یک جلسه فعالیت حاد مقاومتی با شدت متوسط (70 درصد 1RM) و فعالیت حاد مقاومتی شدید (85 درصد 1RM) را در ابتدا و انتهای دو هفته پروتکل تحقیق به اجرا درآوردند و در طول این مدت، مکمل کوآنزیم Q10 (30 میلی گرم/روز) دریافت نمودند. خونگیری بلافاصله پس از فعالیت حاد و مکمل گیری صورت گرفت. شاخص‌های GPX و SOD با روش رنگ سنجی آنزیمی مورد سنجش قرار گرفتند. نتایج با استفاده از آزمون‌های تحلیل واریانس با اندازه گیری مکرر و LSD در سطح 0/05>p استخراج گردید. یافته‌ها: فعالیت حاد مقاومتی متوسط و شدید تغییر معنی داری در فعالیت آنزیم GPX (به ترتیب با 0/06=p و 0/19=p) و SOD (به ترتیب با 0/42=p و 0/61=p) سرم ایجاد نکرد، اما اجرای آن ها پس از دو هفته مکمل یاری کوآنزیم Q10، با افزایش معنی دار فعالیت آنزیم‌‌های SOD (به ترتیب با 0/01=p و 006/0=p) و GPX (p=0/01) همراه بود. در مقایسه تاثیر دو نوع تمرین، تمرین مقاومتی شدید کاهش بیشتر شاخص GPX (p=0/002) را در پی داشت. نتیجه‌گیری: مصرف کوتاه مدت مکمل کوآنزیم Q10 موجب بهبود وضعیت آنتی اکسیدانی افراد غیرفعال شده و می تواند نگرانی افراد تمرین کننده را برای محفوظ ماندن از اثرات مخرب استرس اکسیداتیو پس از تمرینات مقاومتی حاد شدید (بالای 85 درصد 1RM) رفع نماید و به عنوان یک راهبرد تغذیه ای، در کنار سایر مکمل ها گنجانده شود.
کلیدواژه‌ها
عنوان مقاله [English]
Serum changes of glutathione peroxidase and superoxide dismutase in inactive females consumed coenzyme Q10 following of moderate and severe acute resistance training
نویسندگان [English]
- Yeganeh Feizi 1
- Seyed-Hosein Abtahi-Eivary 2
- Mostafa Rezvani 3
1 MSc in Sports Sciences, Faculty of Sports Sciences, University of Birjand, Birjand, Iran.
2 Associate Professor, Department of Laboratory Sciences, Faculty of Allied Medicine, Gonabad University of Medical Sciences, Gonabad, Iran.
3 PhD Student in Exercise Physiology, Faculty of Sports Sciences, Bojnourd University, Bojnourd, Iran.
چکیده [English]
Background and Aim: Moderate to severe exercise trainings may damage most of the body’s tissues, but the use of antioxidant supplements can modulate the exercise-induces oxidative stress. The purpose of the present study was to investigate serum changes of glutathione peroxidase (GPX) and superoxide dismutase (SOD) in sedentary females consumed coenzyme Q10 following a moderate or severe acute resistance training. Materials and Methods: The present research was a semi-experimental study. Twenty seven female students were randomly divided into three groups including two resistance training groups and one control group. Two intervention groups performed one session of resistance training with moderate intensity (70% 1RM) and severe intensity (85% 1RM) at the beginning and end of two weeks of the research protocol. During these two weeks, they orally consumed 30 g/day coenzyme Q10 supplement. Blood sample was taken immediately after the acute training and supplementation. GPX and SOD activity were measured by enzymatic colorimetric method. The results were extracted using repeated measure ANOVA and LSD tests at the p<0.05 significant level. Results: Moderate and severe acute resistance training did not cause a significant changes in serum enzyme GPX (p=0.06, p=0.19 respectively) and SOD (p=0.42 and p=0.61 respectively) activity, but after two weeks of coenzyme Q10 supplementation it was associated with increases in the activity of SOD (p=0.01, p=0.006) and GPX (p=0.01). Comparing the effect of the two types of training, intensive resistance training resulted in a further decrease in GPX index (p=0.002). Conclusion: The use of short-term coenzyme Q10 supplementation improves antioxidant status of sedentary individuals and can alleviate the anxiety of practitioners to avoid the damaging effects of oxidative stress after intense acute resistance training (above 85% 1RM) and can be included with other supplements as a nutritional strategy.
کلیدواژه‌ها [English]
- Acute resistance training
- Superoxide dismutase
- Glutathione peroxidase
- Antioxidant status
Afzalpour, M.E., Saghebjoo, M., Zarban, A., & Jani, M. (2011). Comparison of the effect of one session of acute resistance and aerobic activity on the antioxidant defense system and lipid peroxidation in healthy young men. Journal of Biomotor and Sport Sciences, 3(6), 30-39. [In Persian]
Ahmadi Kakavandi, M., Azizbeigi, K., & Qaisari, S.F. (2019). The effect of increasing resistance training on malondialdehyde concentration and superoxide dismutase activity in inactive elderly women. Journal of Payavard Salamat, 13(2), 151-159 [In Persian]
Almasi, S., Rezvanjoo, B., Shirazi, P., & Khosravi, S.H. (2014). Protective effects of coenzyme Q10 and vitamin C on cysteamine-induced lipid peroxidation and oxidative stress in rats. Veterinary Clinical Research, 5(1), 21-29. [In Persian]
Azizbeigi, K., & Qeysari, S.F. (2019). The Effects of progressive resistance training on malondialdehyde concentration and superoxide dismutase enzyme activity in inactive elderly women. Journal of Payavard Salamat, 13(2), 151-159. [In Persian]
Brzycki, M. (1993). Strength testing—predicting a one-rep max from reps-to-fatigue. Journal of Physical Education, Recreation & Dance, 64(1), 88-90.
Cooke, M., Iosia, M., Buford, T., Shelmadine, B., Hudson, G., & Kerksick C. (2008). Effects of acute and 14-day coenzyme Q10 supplementation on exercise performance in both trained and untrained individuals. Journal of the International Society of Sports Nutrition, 4, 5-8.
Crane, F.L., Hatefi, Y., Lester, R. L., & Widmer, C. (1989). Isolation of a quinone from beef heart mitochondria. Biochimica et Biophysica Acta, 25, 220-221.
Deminice, R., Sicchieri, T., Payão, P.O., & Jordão, A.A. (2010). Blood and salivary oxidative stress biomarkers following an acute session of resistance exercise in humans. International Journal of Sports Medicine, 31(09), 599-603.
Ebrahimi, S., Haghighi, A.H., & Nikkhah, K. (2019). The effect of eight weeks of aerobic-resistance training with Quizum supplementation on antioxidant enzyme activity and lipid peroxidation index in women with multiple sclerosis with regular sexual cycle. Journal of Applied Sport Physiology, 15(30), 8-9. [In Persian]
Ghasemi, E., Afzalpour, M.E., & Saghebjoo, M. (2012). The effect of short-term supplementation of green tea on total antioxidant capacity and lipid peroxidation of young women after a session of intense resistance training. Journal of Isfahan Medical School, 30(202), 1276-1267. [In Persian]
Hosseini Esfahani, F., Asghari, G., Mirmiran, P., Jalali Farahani, S., & Azizi, F. (2009). Reproducibility and relative validity of Food Group Intake in a Food Frequency Questionnaire Developed for the Tehran Lipid and Glucose Study. The Razi Journal of Medical Sciences, 17(71), 41-55. [In Persian]
Jahani, G.H., Firoozrai, M., Matin Homaee, H., Tarverdizadeh, B., Azarbayjani, M.A., & Movaseghi, G.H. (2010). The effect of continuous and regular exercise on erytrocyte antioxidative enzymes activity and stress oxidative in young soccer players. Razi Journal of Medical Sciences, 17(74), 22-32. [In Persian]
Kon, M., Kimura, F., Akimoto, T., Tanabe, K., Murase, Y., & Ikemune, S. (2007). Effect of coenzyme Q10 supplementation on exercise-induced muscular injury of rats. Exercise Immunology Review, 13, 76-88.
Miller, E., Mrowicka, M., Malinowska, K., Mrowicki, J., Saluk-Juszczak, J., & Kędziora, J. (2010). The effects of whole-body cryotherapy on oxidative stress in multiple sclerosis patients. Journal of Thermal Biology, 35(8), 406-410.
Niki, E. (2018). Oxidative stress and antioxidants: Distress or eustress? Free Radical Biology and Medicine, 124(20), 124- 564.
Powers, S. K., Criswell, D., Lawler, J. O. H. N., Martin, D., Lieu, F. K., Ji, L. L., & Herb, R. A. (1993). Rigorous exercise training increases superoxide dismutase activity in ventricular myocardium. American Journal of Physiology-Heart and Circulatory Physiology, 265(6), 2094-2098.
Powers, S.K., & Sen, C.K. (2000). Physiological antioxidants and exercise training. (Sen CK, Packer L,). Hänninen OP. Handbook of oxidants and antioxidants in exercise. 42-221.
Pyne, D.B. (1994). Regulation of neutrophil function during exercise. Sports Medicine, 17(4), 245-258.
Saghebjoo, M., Ghanbari Niaki, A., Fathi, R., & Hedayati, M. (2010). The effect of circular resistance training on the plasma ghrelin level of young women”, Iranian Journal of Endocrinology and Metabolism, 12(5), 535-529. [In Persian]
Samjoo, I.A., Safdar, A., Hamadeh, M.J., Raha, S., & Tarnopolsky, M.A. (2013). The effect of endurance exercise on both skeletal muscle and systemic oxidative stress in previously sedentary obese men. Nutrition & Diabetes, 3(9), e88.
Silva, E.P., Soares, E.O., Malvestiti, R., Hatanaka, E., & Lambertucci, R.H. (2016). Resistance training induces protective adaptation from the oxidative stress induced by an intense-strength session. Sport Sciences for Health, 12(3), 321-328.
Sindhi, V., Gupta, V., Sharma, K., Bhatnagar, S., Kumari, R., & Dhaka, N. (2013). Potential applications of antioxidants – A review. Journal of Pharmacy Research, 7(9), 828–835.
Taati, M., Alirezaei, M., Meshkatalsadat, M.H., Rasoulian, B., Kheradmand, A., & Neamati, S.H. (2011). Antioxidant effects of aqueous fruit extract of Ziziphus jujuba on ethanolinduced oxidative stress in the rat testes. Iranian Journal of Veterinary Research, 12(34), 39-43. [In Persian]
Valado, A., Pereira, L., Tavares, P., & Ribeiro, C. (2007). Effect of the intense anaerobic exercise on nitric oxide and malondialdehyde in studies of oxidative stress. International Journal of Biology and Biomedical Engineering, 1(1), 26-32.
Watson, T.A., MacDonald-Wicks, L.K., & Garg, M.L. (2005). Oxidative stress and antioxidants in athletes undertaking regular exercise training. The International Journal of Sport Nutrition and Exercise Metabolism, 15(2), 131-46.
)