نوع مقاله : مقاله پژوهشی
نویسندگان
1 کارشناس ارشد فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه اصفهان، اصفهان، ایران
2 استاد گروه فیزیولوژی ورزشی ، دانشکده علوم ورزشی، دانشگاه اصفهان، اصفهان، ایران.
چکیده
زمینه و هدف: از آنجا که بسیاری از مسیرهای سلولی و مولکولی درگیر در دیابت ناشناخته هستند، مطالعه حاضر با هدف بررسی تاثیر تمرین هوازی بر ذخایر گلیکوژن کبدی در موش‌های پیش دیابتی به اجرا درآمد روش تحقیق: تعداد 21 سر موش نر هموژن نژاد C57BL/6 به‌طور تصادفی به دو گروه تقسیم شدند. گروه اول موش های تغذیه شده با رژیم غذایی استاندارد (تعداد 7 سر) و گروه دوم موش های تغذیه شده با رژیم غذایی پرچرب (تعداد 14). موش ها به مدت 12 هفته تغذیه شدند و سپس شرایط پیش دیابتی، با آزمون گلوکز ناشتا و آزمون تحمل گلوکز بررسی شد. پس از تائید شرایط پیش دیابتی، موش‌های تغذیه‌شده با رژیم غذایی پرچرب به دو گروه شامل رژیم غذایی پرچرب- بی‌تحرک و رژیم غذایی پرچرب- تمرین هوازی تقسیم گردیدند. موش‌های گروه تمرینی به مدت 10 هفته، پنج روز در هفته و به مدت 45 دقیقه در هر جلسه، بر روی نوار گردان تمرین کردند. 24 ساعت پس از آخرین جلسه تمرینی، موش‌ها کشته شدند و نمونه خونی از قلب موش ها گرفته شد. بافت کبد جدا نیز گردید و برای اندازه‌گیری بیان ژن گلیکوژن سنتاز (که با روش RT-Real time PCR صورت گرفت) در نیتروژن مایع فریز شد. از آزمون t مستقل و تحلیل واریانس یک راهه در سطح p<0/05 جهت تجزیه و تحلیل آماری داده ها استفاده گردید. یافته ها: سطوح گلیکوژن سنتاز در گروه تمرین هوازی نسبت به گروه پیش دیابتی بیشتر بود، بدین معنی که تمرین تأثیر معنی داری (001/0=p) بر بیان گلیکوژن سنتاز داشت همچنین سنجش گلیکوژن کبد نشان داد که میزان ذخایر گلیکوژن کبدی در گروه تمرین نسبت به گروه پیش دیابتی افزایش معنی داری (001/0=p) داشته است. نتیجه گیری: انجام تمرین هوازی می تواند منجر به بهبود شرایط از وضعیت پیش دیابتی به وضعیت سالم شود.
کلیدواژه‌ها
عنوان مقاله [English]
The effect of aerobic training on hepatic glycogen stores in prediabetes mice
نویسندگان [English]
- Milad Abdollahi 1
- Sayed Mohammad Marandi 2
1 MSc in Exercise Physiology, Faculty of Sport Sciences, University of Isfahan, Isfahan, Iran.
2 Full Professor, Department of Exercise Physiology, Faculty of Sport Sciences, University of Isfahan, Isfahan, Iran.
چکیده [English]
Background and Aim: Since many cellular and molecular pathways involve in diabetes are unknown, the aim of the present study was to study the effects of aerobic exercise on glycogen stores in pre-diabetic rats. Materials and Methods: Twenty-one homogenous male C57BL/6 mice were randomly divided into two groups including Mice fed standard diet (n=7) and Mice fed high fat diet (n=14). The mice were fed for 12 weeks and then the pre-diabetic conditions of the mice were assessed by fasting glucose and glucose tolerance tests. After confirmation of pre-diabetic conditions, mice fed a high-fat diet were divided into two groups as high-fat-sedentary diet, and high-fat diet-aerobic exercise. Mice in the training group were trained on a treadmill for 10 weeks, five days a week for 45 minutes in every session. Twenty-four hours after the last training session, the rats were killed and blood was drawn from the rats’ hearts. Liver tissue was isolated and frozen to measure glycogen synthase gene expression (RT-Real time PCR) in liquid nitrogen. The independent t-test and one-way analysis of variance were used for statistical analysis at the p<0.05 level. Results: The glycogen synthase levels in the aerobic exercise group were higher than the prediabetes group and had a significant effect on glycogen synthase expression (p=0.001). Also, liver glycogen showed significantly increase (p=0.001) in the amount of hepatic glycogen stores in the exercise group compared to the prediabetes group. Conclusion: Aerobic exercise can lead to the improvement of the condition from pre-diabetic to healthy condition.
کلیدواژه‌ها [English]
- Pre-diabetes
- High fat diet
- Aerobic training
- Glycogen synthase
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