نوع مقاله : مقاله پژوهشی
نویسندگان
1 دکتری فیزیولوژی ورزشی، بخش علوم ورزشی، دانشکده علوم تربیتی وروانشناسی، دانشگاه شیراز، شیراز، ایران.
2 دانشیار بخش علوم ورزشی، دانشکده علوم تربیتی وروانشناسی، دانشگاه شیراز، شیراز، ایران.
3 استادیار بخش علوم ورزشی، دانشکده علوم تربیتی وروانشناسی، دانشگاه شیراز، شیراز، ایران.
چکیده
زمینه و هدف: پروتئین تنظیم کننده بیوژنز میتوکندریایی گیرنده فعال کننده تکثیر پروکسیزوم گاما-1 آلفا (PGC-1α) به عنوان عامل اصلی مسیر سیگنالینگ در کنترل متابولیسم میتوکندری عمل می‌‌کند. در عین حال، یک عامل رونویسی به نام عامل 2 وابسته به اریتروئید عامل هسته‌‌ای-2 (Nrf2) نیز شناخته شده که با فعال سازی PGC-1α رابطه دارد و تا حدودی مشخص شده است که مسیر یکی از این دو عامل رونویسی، می‌‌تواند بر دیگری تأثیر بگذارد. هدف از تحقیق حاضر بررسی اثر هشت هفته تمرین مقاومتی به همراه مصرف مکمل‌دهی کوآنزیم Q10 بر مقادیر Nrf2، PGC-1α در رت‌‌های نر نژاد اسپراگوداولی بود. روش تحقیق: در این تحقیق تجربی، 36 سر رت به صورت تصادفی به شش گروه مساوی شامل گروه تمرین مقاومتی، دو گروه تمرین مقاومتی و مکمل Q10 با دوزهای 200 و 300 میلی گرم/کیلوگرم وزن، دو گروه مکمل Q10 با دوزهای 200 و 300 میلی گرم/کیلوگرم وزن، و کنترل تقسیم شدند. مکمل به رت‌‌ها در گروه‌‌های تمرین - مکمل و مکمل، گاواژ شد. تمرین مقاومتی شامل سه نوبت با پنج تکرار بود که سه روز در هفته و به مدت هشت هفته اجرا گردید. از آزمون‌‌های تحلیل واریانس یک راهه و توکی در سطح معنی داری 05/0>p برای استخراج نتایج استفاده شد. یافته‌‌ها: بیان Nrf2 و PGC-1α به طور معنی‌‌داری در گروه‌‌های تمرین مقاومتی و تمرین مقاومتی + مکمل (دوز 200 میلی گرم/کیلوگرم) افزایش یافت (0001>p)، در حالی که در گروه تمرین مقاومتی + مکمل با دوز 300 میلی گرم/کیلوگرم (07/0>p) و دو گروه دریافت کننده مکمل (09/0>p) تغییر معنی‌‌داری نکرد. نتیجه گیری: با توجه به اثر افزایشی ترکیب تمرینات مقاومتی همراه با مکمل دهی Q10 (با دوز 200 میلی گرم/کیلوگرم) در بهبود Nrf2 و PGC-1α؛ می‌‌توان این شیوه را در بیوژنز میتوکندریایی موثر دانست.
کلیدواژه‌ها
عنوان مقاله [English]
The effect of eight-weeks of resistance training a long with coenzyme Q10 supplementation on some factors of mitochondrial biogenesis in young male rats
نویسندگان [English]
- Mohammad Javad Shafahi 1
- Mohsen Salesi 2
- Rasoul Rezaei 3
- Farhad Daryanoosh 2
1 PhD in Exercise Physiology, Department of Sport Sciences, School of Education and Psychology, Shiraz University, Shiraz, Iran.
2 Associate Professor, Department of Sport Sciences, School of Education and Psychology, Shiraz University, Shiraz, Iran.
3 Assistant Professor of Exercise Physiology, Department of Sport Sciences, School of Education and Psychology, Shiraz University, Shiraz, Iran.
چکیده [English]
Background and Aim: The mitochondrial biogenesis-regulating protein of the peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) acts as a major signaling pathway for controlling of mitochondrial metabolism. Meanwhile, a transcription factor namely nuclear factor erythroid 2-related factor 2 (Nrf2) is recognized that also associated with PGC1α activation, and it has been shown to some extent to the pathway of one of these two transcription factors can affect the other. The aim of this study was to evaluate the effect of eight weeks of resistance training a long with coenzyme Q10 supplementation on Nrf2 and PGC-1α levels in male Sprague-Dawley rats. Materials and Methods: In this experimental study, 36 rats were randomly divided into six equal groups including resistance training, two resistance training and Q10 supplement (200 and 300 mg/kg/body weight), two supplement (200 and 300 mg/kg/body weight) and control group. The all supplements were applied as a gavage to the exercise-supplement and gavage supplement groups. Resistance training consisted of three sessions and five repetitions which were performed three days a week for eight weeks. One-way analysis of variance and Tukey tests at the significant level of p<0.05 were used to extract the results. Results: Nrf2 and PGC-1α expression increased significantly in resistance training and resistance training+supplement (200 mg/kg) groups (p<0001), while in resistance training group+supplement a dose of 300 mg/kg (p<0.07) and two groups of supplements (p<0.09) no significantly changes were observed. Conclusion: Due to the additive effect of resistance training combined with Q10 supplementation (a dose of 200 mg/kg) in improving Nrf2 and PGC-1α; it can be considered as a effective method in mitochondrial biogenesis.
کلیدواژه‌ها [English]
- Resistance training
- Coenzyme Q10
- Nuclear factor erythroid 2-related factor 2
- Peroxisome proliferator-activated receptor gamma coactivator 1-alpha
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