نوع مقاله : مقاله پژوهشی
نویسندگان
1 کارشناسی ارشد فیزیولوژی ورزش، دانشکده علوم تربیتی و روان شناسی، دانشگاه سیستان و بلوچستان، زاهدان، ایران.
2 استادیار گروه علوم ورزشی، دانشکده علوم تربیتی و روان شناسی، دانشگاه سیستان و بلوچستان، زاهدان، ایران.
3 استادیار گروه تربیت بدنی، دانشکده پزشکی، دانشگاه علوم پزشکی زاهدان، ایران.
چکیده
زمینه و هدف: کنترل چاقی از اجزای مهم بهداشت عمومی به حساب می‌‌آید و فعالیت ورزشی با هدف پیشگیری و درمان چاقی، از راهکارهای مهم توصیه شده است. هدف از پژوهش حاضر بررسی اثر 8 هفته دویدن تداومی بر غلظت سرمی پروتئین شبه متئورین و اینترلوکین-6 (IL-6) در رت های نر مبتلا به سندرم متابولیک بود. روش تحقیق: پژوهش حاضر از نوع تجربی بود. تعداد 24سر رت نر نژاد ویستار 6 هفته‌‌ای با وزن 180-150 گرم، به‌‌طور تصادفی به دو گروه رژیم غذایی استاندارد (SD, n=8) و رژیم غذایی با چربی بالا (HFD, n=16) تقسیم شدند و به مدت 12 هفته تحت رژیم غذایی قبل از ورزش قرار گرفتند. بعد از این دوره، 8 سر رت از گروه (SD) و 8 سر رت از گروه HFD انتخاب شدند تا وضعیت سندرم متابولیک از طریق سنجش سرمی نیمرخ لیپید و انسولین تایید شود. سپس 16 رت گروه (HFD) به طور تصادفی به دو گروه کنترل سندرم متابولیک (Ctr+MetS, n=8) و تمرین تداومی (CT, n=8) تقسیم گردیدند. پروتکل دویدن تداومی هوازی با شدتی معادل 75-65 درصد حداکثر سرعت از هفته اول تا هفته هشتم اجرا شد. در پایان هفته هشتم، مقادیر سرمی پروتئین شبه متئورین و IL-6 با استفاده از کیت های ویژه رت شرکت زلبایو کشور آلمان مورد سنجش قرار گرفتند. آزمون آماری تحلیل واریانس یک راهه و آزمون تعقیبی توکی برای بررسی تغییرات بین گروهی بکار گرفته شد. داده‌‌ها با کمک نرم افزار SPSS نسخه 20 در سطح معنی داری 05/p≤0 تحلیل شدند. یافته‌ها: انجام 8 هفته دویدن تداومی نتوانست بر سطح سرمی متئورین تاثیر معنی داری (افزایش غیرمعنی دار) داشته باشد (09/0=p)؛ با این حال، سطح سرمی IL-6 در گروه سندرم متابولیک افزایش معنی‌‌داری داشت (001/0=p). نتیجه گیری: به نظر می‌‌رسد 8 هفته دویدن تداومی اثرات اندکی بر تنظیم التهاب ناشی از چاقی از طریق تنظیم شاخص سرمی پروتئین شبه متئورین و IL-6داشته باشد.
کلیدواژه‌ها
عنوان مقاله [English]
Effect of 8 weeks of continuous running on serum levels of Meteorine like protein and Interleukin-6 in male rats with syndrome metabolic
نویسندگان [English]
- Zohre Bakhshi 1
- Shila Nayebifar 2
- Abbas Salehikia 2
- Hossein Nakhaei 3
1 MSc of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Sistan and Baluchestan, Zahedan, Iran.
2 Assistant Professor, Department of Sport Sciences, Faculty of Educational Sciences and Psychology, University of Sistan and Baluchestan, Zahedan, Iran.
3 Assistant Professor, Department of Sport Sciences, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
چکیده [English]
Background and Aim: Controlling obesity can be considered as important part of public health; and physical exercise is recommended as an essential intervention for modulate obesity. The aim of this study was to investigate the effect of 8 weeks of continuous running on serum concentrations of Meteorine like protein and Interlukin-6 (IL-6) in male rats with metabolic syndrome. Materials and Methods: The present was an experimental study. 24 Wistar male rats, with age 6 weeks old and weight 150-180 gr randomly divided into two groups including standard diet (SD, n=8) and high fat diet (HFD, n=16). They were then placed on a pre-exercise diet for 12 weeks. After this period, 8 rats from each groups of SD and HFD were selected to determine syndrome metabolic state through lipid profile and Insulin serum content measurements. Moreover, 16 rats from HDF group were divided randomly into two groups including metabolic syndrome control (Ctr+MetS, n=8) and continuous running (CT, n=8). The aerobic continuous running protocol, included 65-75% of maximum speed performed from week 1 to 8 At the end of intervention, serum levels of Meteorine like protein and IL-6 were assessed through specific rats ZellBio kits. One way ANOVA and Tukey post hoc tests were used to determine mean differences between groups. Data were analyzed using SPSS version 20 and the significance level was set as p≤0.05. Results: Eight weeks of continuous running didn’t indicate any significant effect (insignificant increase) on serum meteorine level (p=0.09) but showed a significant increase on interleukin 6 in metabolic syndrome group (p=0.001). Conclusion: It seems that 8 weeks of continuous running have a little impact on inflammation induced obesity through modulating of meteorine-like protein and IL-6.
کلیدواژه‌ها [English]
- Continuous exercise
- Meteorine-like protein
- Interleukin-6
- Metabolic syndrome
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