نوع مقاله : مقاله پژوهشی
نویسنده
استادیار، گروه علوم ورزشی، واحد شیروان، دانشگاه آزاد اسلامی، شیروان، ایران
چکیده
زمینه و هدف: گیرنده های بتا و گامای فعال شونده با تکثیرکننده پراکسیزوم (PPARβ و PPARγ) محرک بیان ژن های درگیر در انتقال معکوس کلسترول و دفاع آنتی اکسیدانی می باشند، از این رو بیان ژن و افزایش فعالیت این دو ماده می تواند یکی از مکانیزم های مطلوب در پیشگیری از آتروسکلروز باشد. هدف از انجام تحقیق حاضر بررسی تأثیر تمرینات شدید اینتروال (HIIT) و کم شدت تداومی (LICT) پس از رژیم غذایی پرچرب (HFD) بر بیان ژن های PPARβ و PPARγ در موش های نر ویستار بود. روش تحقیق: این تحقیق تجربی شامل دو مرحله چاق کردن با HFD (13 هفته) و تمرین (12 هفته، 5 جلسه در هفته) بود. پس از مرحله چاق کردن، آزمودنی ها به 3 گروه کنترل، تمرین HIIT و تمرین LICT تقسیم شدند. پس از پایان تمرینات، بیان ژن های PPARβ و PPARγ با تکنیک واکنش زنجیره پلیمراز در بافت کبد اندازه گیری شد. با استفاده از آزمون های کروسکاوالیس و یومن ویتنی در سطح معنی داری 05/0≥P نتایج استخراج گردید. یافته ها: بیان ژن های PPARβ و PPARγ بین گروه های کنترل با HIT (008/0 p=) و LICT (008/0 p=) و بین گروه HIT با LICT (008/0 p=) متفاوت بود؛ به گونه ای که بیشترین میزان بیان ژن های PPARβ و PPARγ در گروه HIIT و کمترین میزان آن در گروه کنترل به دست آمد. نتیجه گیری: اجرای منظم HIIT و LICT هردو، با افزایش بیان ژن های PPARβ و PPARγ می توانند در کاهش خطر سکته قلبی مؤثر باشند، با این حال استفاده از HIIT اثربخش تر خواهد بود.
کلیدواژه‌ها
عنوان مقاله [English]
eroxisome proliferator-activated receptors beta and gamma (PPARβ and PPARγ) genes expression following exercise trainings and high fat diet in male Wistar rats
نویسنده [English]
- Mohsen Jafari
Assistant Professor, Department of Sport Sciences, Shirvan Branch, Islamic Azad University, Shirvan, Iran.
چکیده [English]
Background and Aim: Peroxisome proliferator-activated receptors beta and gamma (PPARβ and PPARγ) are stimulator of genes expression involved in reverse cholesterol transport and anti-oxidant defense, thus gene expression and activity of this substance can be one of beneficial mechanisms in prevention of atherosclerosis. The aim of this study was to investigate the effect of high intensity interval training (HIIT) and low intensity continuous training (LICT) after high fat diet (HFD) on PPARβ and PPARγ genes expression in male Wistar rats. Materials and Methods: This experimental study involved two phases of fattening (13 weeks) and training (12 weeks, 5 sessions per week). After fattening phase, subjects assigned into three groups as: control, HIIT and LICT. Analysis of PPARβ and PPARγ genes expression was done using polymerase chain reaction technique after trainings. Results were obtained using Kruskal–Wallis and Mann–Whitney U tests in level of P≤0.05. Results: The PPARβ and PPAR γ genes expression were different between control group with HIIT (P=0.008) and LICT (P=0.008) and between HIIT with LICT (P=0.008); so that higher levels of PPARβ and PPAR γ genes expression observed in HIIT group and lower levels were in control group. Conclusion: Results of this study showed that regular HIIT and LICT through elevation of PPARβ and PPARγ genes expression may be effective in reduction of heart attack risk and HIIT are more effective than LICT.
کلیدواژه‌ها [English]
- Peroxisome proliferator activated receptor
- High intensity interval training
- Continuous training
- High fat diet
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