نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشیار گروه تربیت بدنی و علوم ورزشی، دانشگاه پیام نور، تهران، ایران.
2 کارشناس ارشد، گروه تربیت بدنی و علوم ورزشی، دانشگاه پیام نور، تهران، ایران.
3 استادیار گروه تربیت بدنی و علوم ورزشی، دانشگاه پیام نور، تهران، ایران.
چکیده
زمینه و هدف: با توجه به اهمیت پیشگیری از بروز بیماریهای قلبی و نیز فقدان اطلاعات لازم در خصوص تأثیر تمرینهای ورزشی بر بیان ژنهای آنژیوپویتین-1 وکانستاتین، هدف مطالعه حاضر بررسی تاثیر چهار شیوه تمرینی بر بیان ژنهای آنژیوپویتین-1 وکانستاتین در بطن چپ موشهای صحرایی نژاد ویستار بود. روش تحقیق: بدین منظور تعداد 40 سر موش صحرایی نر نژاد ویستار 8 هفتهای با میانگین وزنی33±237 گرم به طور تصادفی به 5 گروه (8 سر در هر گروه) شامل گروههای کنترل، تمرین تداومی با شدت متوسط (MIT)، تمرین تداومی شدید (HIT)، تمرین هوازی تناوبی شدید (HIIT) و تمرین شنا با شدت متوسط (MIST) تقسیم شدند. برنامههای تمرینی در گروههای تجربی به مدت 8 هفته و 5 روز در هفته انجام شد. 48 ساعت پس از آخرین جلسة تمرینی، نمونهبرداری از بافت قلب انجام گرفت. بیان ژنهای آنژیوپویتین-1 وکانستاتین در بافت قلب با روش PCR تعیین شد. جهت تجزیه و تحلیل دادهها از آزمونهای تحلیل واریانس یکطرفه و توکی در سطح معنی داری 05/0≥p استفاده گردید. یافته ها: بیان ژنهای آنژیوپویتین-1 و کانستاتین در هر یک از گروههای مداخله در مقایسه با گروه کنترل، به طور معنیداری (001/0p=) کاهش یافت. در مقایسه چهار نوع تمرین مشخص گردید که بیان آنژیوپویتین-1 در گروهMIST نسبت به سایر گروههای تمرینی و بیان کانستاتین در گروههای HIT، MIT وMIST نسبت به گروه HIIT کاهش معنیدار بیشتری (001/0p=) داشته است. نتیجه گیری: هر چهار شیوه تمرینی توانست با بهبود بیان ژنهای مورد مطالعه، تغییرات مطلوبی در کاهش پیامدهای ناشی از آسیب بافت قلبی ایجاد نماید؛ با این حال تمرینات شنا با شدت متوسط با توجه به ویژگی عدم تحمل وزن، تأثیرات بیشتری دارد. در این زمینه به تحقیقات بیشتری نیاز است.
کلیدواژه‌ها
عنوان مقاله [English]
The effect of four types of exercise training protocols on the angiopoietin-1 and canstatin expression in the left ventricle of Wistar rats
نویسندگان [English]
- Mohammadreza Asad 1
- Elahe Fallahzadeh 2
- Ali Barzegari 3
1 Associate Professor, Department of Physical Education and Sport Sciences, Payame Noor University, Tehran, Iran.
2 MSc, Department of Physical Education and Sport Sciences, Payame Noor University, Tehran, Iran.
3 Assistant Professor, Department of Physical Education and Sport Sciences, Payame Noor University, Tehran, Iran.
چکیده [English]
Background and Aim: Considering the importance of prevention of risk factors in heart disease and lack of information about the effect of exercise training on angiopoietin 1 and canstatin expression, therefore the aim of this study was to investigate the effect of four types of exercise training protocols on the angiopoietin-1 and canstatin expression in left ventricle of Wistar rats. Materials and Methods: For this purpose, 40 male Wistar rats with age of 8-weeks and an average weight of 237±33 grams were randomly divided into five groups (8 rats in each group), including control, moderate intensity training (MIT), high intensity training (HIT), high intensity interval training (HIIT) and moderate-intensity swimming training groups (MIST). The training programs in experimental groups were performed for 8 weeks and 5 days a week. 48 hours after the last training session, the sampling was taken from cardiac tissue. Then, the angiopoietin-1 and canstatin expression were determined by PCR method. To analyze data, one way ANOVA and tukeywere used at a significance level of p≤0.05. Results: Angiopoietin-1 and canstatin expressions in each intervention groups were significantly decreased compared to the control group (p=0.001). The comparison of four types of training showed that angiopoietin-1 expression was significantly decreased only in MIST group as compared to the other training groups and canstatin expression was significantly decreased in HIT, MIT and MIST groups when compared to the HIIT group (p=0.001). Conclusion: All of the four types of exercise training methods were able to reduce the expression of the studied genes,and make favorable changes in reducing the consequences of cardiac tissue injury. However, moderate-intensity swimming training seem to have more favorable effects with regard to non-weight bearing property, although further research is needed.
کلیدواژه‌ها [English]
- Angiopoietin-1
- Canstatin
- Exercise training
- Heart tissue
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