نوع مقاله : مقاله پژوهشی
نویسندگان
1 کارشناس ارشد فیزیولوژی ورزشی، گروه تربیت بدنی و علوم ورزشی، واحد گچساران، دانشگاه آزاد اسلامی، گچساران، ایران.
2 استادیار گروه تربیت بدنی و علوم ورزشی، واحد گچساران، دانشگاه آزاد اسلامی، گچساران، ایران.
چکیده
زمینه و هدف: کاهش استروژن در دوره یائسگی میتواند منجر به افزایش فشار اکسایشی و کاهش آنزیمهای ضد اکسایشی گردد، اما فعالیت ورزشی و تغذیه دو عامل موثر در بهبود این شرایط گزارش شده اند. هدف تحقیق حاضر بررسی تاثیر تمرینات استقامتی و مصرف کروسین بر سطوح سرمی آنزیم های کاتالاز و گلوتاتیون پراکسیداز موش های صحرایی ماده ی فاقد تخمدان بود. روش تحقیق: تعداد 36 سر موش صحرایی ماده فاقد تخمدان با سن 8 تا 10 هفته ای به 4 گروه شامل گروه کنترل، تمرین استقامتی، مصرف کروسین، و تمرین استقامتی همراه با مصرف کروسین (هر گروه 9 سر موش) تقسیم شدند. در طول مدت 8 هفته، گروههای تمرین استقامتی و تمرین استقامتی همراه با مصرف کروسین، برنامه تمرین را 5 جلسه در هفته به اجرا درآوردند و گروه های مصرف کروسین، و تمرین استقامتی همراه با مصرف کروسین روزانه 25 میلی گرم بر کیلوگرم وزن بدن کروسین به صورت درون صفاقی مکمل دهی شدند. با استفاده از روش طول موج، سطوح آنزیم ها اندازه گیری شد و با استفاده از روش آماری تحلیل واریانس یک طرفه و آزمون توکی (در سطح 05/0p < ) نتایج استخراج گردید. یافته ها: تمرین استقامتی (به ترتیب با 03/0>p و 001/0>p)، مصرف کروسین (به ترتیب با001/0>p و 001/0>p)، و تمرین استقامتی + مصرف کروسین (به ترتیب با 001/0>p و 001/0>p)؛ اثر معنی داری بر افزایش سطوح سرمی کاتالاز و گلوتاتیون پراکسیداز موش های صحرایی ماده فاقد تخمدان داشتند؛ و در این بین، تاثیر تمرین+ مصرف کروسین بر سطوح آنزیم ها بیشتر بود (001/0>p). نتیجه گیری: تمرین استقامتی همراه با مصرف کروسین، با افزایش سطوح سرمی کاتالاز و گلوتاتیون پراکسیداز، موش های صحرایی ماده فاقد تخمدان را در مقابل فشار اکسایشی محافظت می کند.
کلیدواژه‌ها
عنوان مقاله [English]
The effect of endurance training and crocin consumption on serum levels of catalase and glutathione peroxidase in ovarictomized rats
نویسندگان [English]
- Simin Ghabezi 1
- Ali Khajehlandi 2
- Amin Mohammadi 2
1 MSc in Exercise Physiology, Department of Physical Education & Sport Sciences, Gachsaran Branch, Islamic Azad University, Gachsaran, Iran.
2 Assistant Professor, Department of Physical Education & Sport Sciences, Gachsaran Branch, Islamic Azad University, Gachsaran, Iran.
چکیده [English]
Background and Aim: Estrogen reduction during menopause can lead to an increase in oxidative stress and decrease in antioxidant enzymes, but physical activity and nutrition can be considered as two important factors for improving these conditions. The purpose of the current study was to investigate the effect of endurance training and crocin consumption on serum levels of catalase and glutathione peroxidase enzymes medulla in ovariectomized rats. Materials and Methods: Thirty six female ovariectomized rats with average ages ranging from 8 to 10 weeks were divided into 4 groups (n=9) including control, endurance training, crocin consumption and endurance training and crocin consumption groups. During 8 weeks period, the groups endurance training and endurance training plus crocin consumption were subjected to 5 training sessions per week while the crocin consumption and endurance training plus crocin consumption groups were received 25 mg/kg body weight crocin peritoneally. Enzyme levels were measured by the wavelength method and to analyze data one-way analysis of variance (ANOVA) along with Tukey’s post hoc test were used at the significant level of p < 0.05. Results: Endurance training (p<0.03 and p<0.001, respectively), crocin consumption (p<0.001 and p<0.001, respectively), and endurance training plus crocin consumption (p < 0.001 and p<0.001, respectively) had a significant increase on the serum catalase and glutathione peroxidase activity in ovariectomized rats. However, endurance training plus crocin consumption had a greater effect on increasing enzyme levels (p<0.001). Conclusion: Endurance training along with crocin consumption can have a protective effect against oxidative stress by increasing serum levels of catalase and glutathione peroxidase medulla in ovariectomized rats.
کلیدواژه‌ها [English]
- Endurance training
- Antioxidant enzymes
- Estrogen
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