نوع مقاله : مقاله پژوهشی
نویسندگان
- سامان حاجی زاده انور 1
- محمدرضا کردی 2
- پریسا پورنعمتی 3
- سارا فرج نیا 4
- نیما قره داغی 5
- محمدرضا رحمتی 1
1 دانشجوی دکتری فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران.
2 دانشیار گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران.
3 استادیار گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران.
4 کارشناس ارشد فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران.
5 دکتری فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران.
چکیده
زمینه و هدف: عامل کمکی رونویسی پروکسی زومی 1-آلفا (PGC-1α) در زیست‌زایی میتوکندریایی و آیریزین در رگ‌زایی، عضله‌زایی و سلامتی بسیار مهم می‌باشند. هدف این تحقیق، بررسی تاثیر غوطهوری در آب سرد پس از فعالیت سرعتی تکراری، بر آیریزین و PGC-1α بود. روش تحقیق: از بین 50 ورزشکار لیگ برتر فوتبال تهران، 20 مرد (میانگین سنی 1/67±23/50 سال)، به شکل تصادفی ساده انتخاب و پس از فعالیت سرعتی تکراری شدید، 10 آزمودنی در آب سرد 14 درجه سانتی گراد (CWI) قرار گرفتند و باقی آن ها (CON) روی صندلی نشستند. قبل و پس از فعالیت، غوطهوری آب سرد یا استراحت و 24 ساعت بعد؛ خونگیری به عمل آمد. آیریزین و PGC-1α سرمی با روش الایزا با کیت شرکت زل بایو آلمان سنجیده شدند. برای تعیین طبیعی بودن توزیع داده‌ها از آزمون شاپیرو-ویلک و برای تعیین اختلاف احتمالی میانگین‌ها در هر یک از گروه‌ها در زمان‌های مختلف و بررسی اثر تعاملی روش بازیافت و زمان‌های مختلف اندازه‌گیری؛ از روش آماری تحلیل واریانس با اندازه‌گیری مکرر استفاده شد و سطح معنی داری 0/05≥p در نظر گرفته شد. یافته‌ها: عامل زمان بر PGC-1α اثر معنی دار داشت [27/0=2pη، 001/0=p، 52/6=(51 ، 3 )F]، اما اثر گروه [38/0=p ، 79/0=(17، 1) F] و اثر ترکیبی (زمان × گروه) معنی دار نبود [21/0=p، 53/1=(51، 3)F]. عامل PGC-1α در هر دو گروه (CWI و CON) پس از فعالیت افزایش معنی دار داشت (004/0=p)، اما پس از غوطه وری یا استراحت، تغییر معنی دار نداشت (00/1=p). همچنین 24 ساعت بعد، تغییرات آن معنی دار نبود (1/00=p). به علاوه، عامل زمان بر مقادیر آیریزین اثر معنی داری داشت [47/0= 2pη، 001/0p<، 38/15= (51، 3)F]، اما اثر گروه [49/0=p، 48/0= F(17، 1)] و اثر ترکیبی (زمان × گروه) معنی دار نبود [14/0=p، 91/1=(51، 3)F]. همچنین آیریزین در هر دو گروه پس از فعالیت افزایش معنی دار داشت (0/001>p)، اما مقادیر آن پس از غوطه وری یا استراحت، تغییر معنی دار نداشت (06/0=p). به علاوه، 24 ساعت بعد تغییرات این شاخص معنی دار نبود (1/00=p). نتیجه گیری: به نظر فعالیت سرعتی تکراری می تواند با افزایش PGC-1α و آیریزین به فرآیندهای مثبت سلولی کمک نماید.
کلیدواژه‌ها
عنوان مقاله [English]
The effects of cold water immersion post repeated sprint activity on serum PGC-1α and irisin in young active men
نویسندگان [English]
- Saman Hadjizadeh Anvar 1
- Mohammadreza Kordi 2
- Parisa Pournemati 3
- Sara Farajnia 4
- Nima Gharadaghi 5
- Mohammadreza Rahmati 1
1 PhD Student in Exercise Physiology, Department of Exercise Physiology, Faculty of Physical Education & Sport Sciences, University of Tehran, Tehran, Iran.
2 Associate Professor, Department of Exercise Physiology, Faculty of Physical Education & Sport Sciences, University of Tehran, Tehran, Iran.
3 Assistant Professor, Department of Exercise Physiology, Faculty of Physical Education & Sport Sciences, University of Tehran, Tehran, Iran.
4 MSc In Exercise Physiology, Department of Exercise Physiology, Faculty of Physical Education & Sport Sciences, University of Tehran, Tehran, Iran.
5 MSc In Exercise Physiology, Department of Exercise Physiology, Faculty of Physical Education & Sport Sciences, University of Tehran, Tehran, Iran.
چکیده [English]
Background and Aim: It is mentioned a critical role for peroxisome proliferator-activated receptor gamma coactivator 1α (PGC-1α) in mitochondrial biogenesis, and for irisin in angiogenesis, myogenesis and health. The purpose of this study was to investigate the effect of cold-water immersion (CWI) post repeated sprint activity (RSA) on irisin & PGC-1α. Materials and Methods: Among 50 soccer players recruited from Tehran premier league, 20 men (age 23.5±1.67 yrs) were selected randomly to this study and after the RSA, 10 participants immersed in cold water (14°C) and 10 others set on a chair passively. Blood sampling was taken before and after RSA, after CWI or passive rest and after 24 hours. Serum irisin & PGC-1α were assessed through ELIZA kit of ZelBio, Germany. Shapiro-Wilk test was performed to determine data normality and to determine possibly differences between means in each group and in different times, analysis of variance test with repeated measures was applied at the significant level of p≤0.05. Results: The time factor had a significant effect on PGC-1α levels [F(3,51)=6.52, p=0.001, pη2=0.27], but the group effect [F(1,17)=0.79, p=0.38] and time - group interaction [F(3,51)=1.53, p=0.21] was not significant. PGC-1α had a significant increases after the RSA in both groups (p=0.004), but its changes were not significant after CWI or rest (p=1.00). In addition, PGC-1α changes was not significant after 24h (p=1.00). Moreover, the time factor had a significant effect on irisin levels [F(3,51)=15.38, p<0.001, pη2=0.47], but the group effect [F(1,17)=0.48, p=0.49] and the time – group interaction [F(3,51)=1.91, p=0.14] were nor significant. In other hand, irisin had a significant increases after the RSA in both groups (p<0.001), but its changes were not significant after CWI or rest (p=0.06). Further, the changes of irisin was not significant after 24h (p=1.00). Conclusion: It seems that the RSA could improve cellular processes through PGC-1α & irisin elevation.
کلیدواژه‌ها [English]
- Cold water immersion
- Repeated sprint activity
- PGC-1α
- Irisin
Allan, R., Sharples, A. P., Close, G. L., Drust, B., Shepherd, S. O., Dutton, J., ... & Gregson, W. (2017). Postexercise cold water immersion modulates skeletal muscle PGC-1α mRNA expression in immersed and nonimmersed limbs: evidence of systemic regulation. Journal of Applied Physiology, 123(2), 451-459.
Baar, K., Wende, A. R., Jones, T. E., Marison, M., Nolte, L. A., Chen, M. A. Y., ... & Holloszy, J. O. (2002). Adaptations of skeletal muscle to exercise: rapid increase in the transcriptional coactivator PGC-1. The Federation of American Societies For Experimental Biology Journal, 16(14), 1879-1886.
Bartlett, J. D., Louhelainen, J., Iqbal, Z., Cochran, A. J., Gibala, M. J., Gregson, W., ... & Morton, J. P. (2013). Reduced carbohydrate availability enhances exercise-induced p53 signaling in human skeletal muscle: implications for mitochondrial biogenesis. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 304(6), R450-R458.
Boström, P., Wu, J., Jedrychowski, M. P., Korde, A., Ye, L., Lo, J. C., ... & Kajimura, S. (2012). A PGC1-α dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature, 481(7382), 463 -468.
Boutcher, S. H. (2011). High-intensity intermittent exercise and fat loss. Journal of Obesity, 2011, 1-10.
Cantó, C., & Auwerx, J. (2010). AMP-activated protein kinase and its downstream transcriptional pathways. Cellular and Molecular Life Sciences, 67(20), 3407-3423.
Dinas, P. C., Lahart, I. M., Timmons, J. A., Svensson, P. A., Koutedakis, Y., Flouris, A. D., & Metsios, G. S. (2017). Effects of physical activity on the link between PGC-1a and FNDC5 in muscle, circulating Ιrisin and UCP1 of white adipocytes in humans: A systematic review. F1000Research, 6, 286.
Dulian, K., Laskowski, R., Grzywacz, T., Kujach, S., Flis, D. J., Smaruj, M., & Ziemann, E. (2015). The whole body cryostimulation modifies irisin concentration and reduces inflammation in middle aged, obese men. Cryobiology, 71(3), 398-404.
Egan, B., Carson, B. P., Garcia‐Roves, P. M., Chibalin, A. V., Sarsfield, F. M., Barron, N., ... & O’Gorman, D. J. (2010). Exercise intensity‐dependent regulation of peroxisome proliferator‐activated receptor γ coactivator‐1α mRNA abundance is associated with differential activation of upstream signalling kinases in human skeletal muscle. The Journal of Physiology, 588(10), 1779-1790.
Elsen, M., Raschke, S., & Eckel, J. (2014). Browning of white fat: does irisin play a role in humans? Journal of Endocrinology, 222(1), R25-R38.
Girard, O., Mendez-Villanueva, A., & Bishop, D. (2011). Repeated-sprint ability—Part I. Sports Medicine, 41(8), 673694.
Gregson, W., Black, M. A., Jones, H., Milson, J., Morton, J., Dawson, B., ... & Green, D. J. (2011). Influence of cold water immersion on limb and cutaneous blood flow at rest. The American Journal of Sports Medicine, 39(6), 13161323.
Hecksteden, A., Wegmann, M., Steffen, A., Kraushaar, J., Morsch, A., Ruppenthal, S., ... & Meyer, T. (2013). Irisin and exercise training in humans–results from a randomized controlled training trial. BMC Medicine, 11(1), 235.
Heubert, R. A. P., Billat, V. L., Chassaing, P., Bocquet, V., Morton, R. H., Koralsztein, J. P., & Di Prampero, P. E. (2005). Effect of a previous sprint on the parameters of the work-time to exhaustion relationship in high intensity cycling. International Journal of Sports Medicine, 26(07), 583-592.
Higgins, T. R., Cameron, M. L., & Climstein, M. (2012). Evaluation of passive recovery, cold water immersion, and contrast baths for recovery, as measured by game performances markers, between two simulated games of rugby union. Journal of Strength and Conditioning Research, 1-24.
Ihsan, M., Markworth, J. F., Watson, G., Choo, H. C., Govus, A., Pham, T., ... & Abbiss, C. R. (2015). Regular postexercise cooling enhances mitochondrial biogenesis through AMPK and p38 MAPK in human skeletal muscle. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 309(3), R286-R294.
Ihsan, M., Watson, G., Choo, H. C., Lewandowski, P., Papazzo, A., Cameron-Smith, D., & Abbiss, C. R. (2014). Postexercise muscle cooling enhances gene expression of PGC-1α. Medicine & Science in Sports & Exercise, 46 (10), 1900-1907.
Impey, S. G., Hammond, K. M., Shepherd, S. O., Sharples, A. P., Stewart, C., Limb, M., ... & Close, G. L. (2016). Fuel for the work required: a practical approach to amalgamating train‐low paradigms for endurance athletes. Physiological Reports, 4(10), e12803.
Joo, C. H. (2015). Effect of post -exercise cold water immersion on molecilar on responses to high intesity intermittent exercise. Doctoral dissertation, Liverpool John Moores University.
Kwaśniewska, M., Kostka, T., Jegier, A., Dziankowska-Zaborszczyk, E., Leszczyńska, J., Rębowska, E., ... & Drygas, W. (2016). Regular physical activity and cardiovascular biomarkers in prevention of atherosclerosis in men: a 25-year prospective cohort study. BMC Cardiovascular Disorders, 16(1), 65.
Lee, P., Linderman, J. D., Smith, S., Brychta, R. J., Wang, J., Idelson, C., ... & Kebebew, E. (2014). Irisin and FGF21 are cold-induced endocrine activators of brown fat function in humans. Cell Metabolism, 19(2), 302-309.
Lombardi, G., Ziemann, E., & Banfi, G. (2017). Whole-body cryotherapy in athletes: from therapy to stimulation. An updated review of the literature. Frontiers in Physiology, 8, 258.
Manfredi, L. H., Zanon, N. M., Garófalo, M., Navegantes, L. C., & Kettelhut, I. (2013). Effect of short-term cold exposure onskeletal muscle protein breakdown in rats. Journal of Applied Physiology, 115(10), 1496-1505.
May, M. J., & Ghosh, S. (1998). Signal transduction through NF-κB. Immunology Today, 19(2), 80-88.
Melhim, A. (2001). Aerobic and anaerobic power responses tothe practice of taekwon-do. British Journal of Sports Medicine, 35(4), 231-234.
Miura, S., Kawanaka, K., Kai, Y., Tamura, M., Goto, M., Shiuchi, T., ... & Ezaki, O. (2007). An increase in murine skeletal muscle peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) mRNA in response to exercise is mediated by β-adrenergic receptor activation. Endocrinology, 148(7), 3441-3448.
Norheim, F., Langleite, T. M., Hjorth, M., Holen, T., Kielland, A., Stadheim, H. K., ... & Drevon, C. A. (2014). The effects of acute and chronic exercise on PGC‐1α, irisin and browning of subcutaneous adipose tissue in humans. The Federation of European Biochemical Societies Journal, 281(3), 739-749.
Novelle, M. G., Contreras, C., Romero-Picó, A., López, M., & Diéguez, C. (2013). Irisin, two years later. International Journal of Endocrinology, 2013, 1-8.
Oliveira, R. L., Ueno, M., de Souza, C. T., Pereira-da-Silva, M., Gasparetti, A. L., Bezzera, R. M., ... & Velloso, L. A. (2004). Cold-induced PGC-1α expression modulates muscle glucose uptake through an insulin receptor/Aktindependent, AMPK-dependent pathway. American Journal of Physiology-Endocrinology and Metabolism, 287(4), E686 -E695.
Pedersen, B. K. (2012). A muscular twist on the fate of fat. New England Journal of Medicine, 366(16), 1544-1545.
Puigserver, P., & Spiegelman, B. M. (2003). Peroxisome proliferator-activated receptor-γ coactivator 1α (PGC-1α): transcriptional coactivator and metabolic regulator. Endocrine Reviews, 24(1), 78-90.
Segsworth, B. M. (2015). Acute sprint interval exercise induces a greater FGF-21 response in comparison to work-matched continuous exercise. Thesis Degree in Master of Science, Graduate Kinesiology, University of Western Ontario.
Slivka, D., Heesch, M., Dumke, C., Cuddy, J., Hailes, W., & Ruby, B. (2013). Effects of post-exercise recovery in a cold environment on muscle glycogen, PGC-1α, and downstream transcription factors. Cryobiology, 66(3), 250255.
Slivka, D. R., Dumke, C. L., Tucker, T. J., Cuddy, J. S., & Ruby, B. (2012). Human mRNA response to exercise and temperature. International Journal of Sports Medicine, 33(02), 94-100.
Taylor, C. W., Ingham, S. A., & Ferguson, R. A. (2016). Acute and chronic effect of sprint interval training combined with postexercise blood‐flow restriction in trained individuals. Experimental Physiology, 101(1), 143-154.
Tsuchiya, Y., Ando,D., Goto, K., Kiuchi, M., Yamakita, M., & Koyama, K. (2014). High-intensity exercise causes greater irisin response compared with low-intensity exercise under similar energy consumption. The Tohoku Journal of Experimental Medicine, 233(2), 135-140.
Vandewalle, H., Peres, G., Heller, J., Panel, J., & Monod, H. (1987). Force-velocity relationship and maximal power on a cycle ergometer. European Journal of Applied Physiology and Occupational Physiology, 56(6), 650-656.
Villena, J. A. (2015). New insights into PGC-1 coactivators: redefining their role in the regulation of mitochondrial function and beyond. The Federation of European Biochemical Societies Journal, 282(4), 647-672.
Widegren, U., Jiang, X. J., Krook, A., Chibalin, A. V., Björnholm, M., Tally, M., ... & Zierath, J. R. (1998). Divergent effects of exercise on metabolic and mitogenic signaling pathways in human skeletal muscle. The Federation of American Societies for Experimental Biology Journal, 12(13), 1379-1389.
Wilcock, I. M., Cronin, J. B., & Hing, W. A. (2006). Physiological response to water immersion. Sports Medicine, 36 (9), 747-765.
Yeargin, S. W., Casa, D. J., McClung, J. M., & Knight, J. C. (2006). Body cooling between two bouts of exercise in the heat enhances subsequent performance. Journal of Strength and Conditioning Research, 20(2), 383.
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