نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد فیزیولوژی ورزش، دانشکده علوم انسانی، دانشگاه لرستان. خرم‌آباد، ایران.

2 دانشیار گروه تربیت بدنی و علوم ورزشی، دانشکده علوم انسانی، دانشگاه لرستان، خرم‌آباد، ایران.

3 استاد گروه تربیت بدنی و علوم ورزشی، دانشکده علوم انسانی، دانشگاه لرستان، خرم‌آباد، ایران.

4 دانشجوی دکترای فیزیولوژی ورزش، دانشکده علوم انسانی، دانشگاه لرستان خرم‌آباد، ایران.

چکیده

زمینه و هدف: دیابت با ایجاد التهاب در قلب، زمینه ابتلاء به بیماری‌های قلبی عروقی را فراهم می‌کند. هدف از پژوهش حاضر، بررسی اثر شش هفته تمرین هوازی پیشرونده بر بیان ژن های عامل رشد اندوتلیوم عروقی (vegf) و عامل نکروزی تومور آلفا (tnfα) قلب رت‌های نر ویستار دیابتی است. روش تحقیق: تعداد 40 سر رت نر نژاد ویستار (میانگین وزن 92/12± 09/253 گرم، سن 10-8 هفته) به طور تصادفی به چهار گروه کنترل، دیابتی، تمرین، و دیابت + تمرین؛ تقسیم شدند. القای دیابت از طریق تزریق درون صفاقی محلول استروپتوزتوسین به میزان 50 میلی‌گرم برکیلوگرم انجام پذیرفت. پروتکل تمرین شامل شش هفته تمرین هوازی پیشرونده، پنج جلسه در هفته بر روی نوار گردان بود. میزان بیان ژن vegf و tnfα بافت بطن چپ به روش Real -Time PCR اندازه‌گیری شد. با استفاده از آزمون تحلیل واریانس یک‌ راهه و آزمون توکی، در سطح معنی‌داری 05/0>p؛ نتایج استخراج گردید. یافته‌ها: بیان ژن های vegf و tnfα بطن چپ قلب در گروه دیابتی شده نسبت به گروه سالم، به ترتیب کاهش (0001/0>p) و افزایش یافت (0001/0>p). از طرف دیگر، بیان ژن های vegf و tnfα بطن چپ قلب گروه دیابت+ تمرین نسبت به گروه دیابت، به ترتیب افزایش (003/0=p) و کاهش یافت (0001/0>p) پیدا کرد. نتیجه‌گیری: تمرین هوازی پیشرونده، می‌تواند به عنوان یک شیوه مناسب غیر دارویی برای بهبود التهاب و رگ زایی در نمونه‌های دیابتی در نظر گرفته شود.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of six weeks of progressive aerobic training on vascular endothelium growth factor and tumor necrosis factor-alpha of the heart of diabetic male Wistar rats

نویسندگان [English]

  • Mohsen Erfanikia 1
  • Mohammad Fathi 2
  • Masoud Rahmati 3
  • Eftekhar Mohammadi 4

1 MSc in Exercise Physiology, Faculty of Human Sciences, Lorestan University, Khorramabad, Iran.

2 Associate Professor at Dept. of Physical Education and Sport Sciences, Faculty of Human Sciences, Lorestan University, Khorramabad, Iran.

3 Professor at Dept. of Physical Education and Sport Sciences, Faculty of Human Sciences, Lorestan University, Khorramabad, Iran.

4 Ph.D. Student in Exercise Physiology, Faculty of Human Sciences, Lorestan University, Khorramabad, Iran.

چکیده [English]

Background and Aim: Diabetes causes inflammation in the heart, which predisposes to cardiovascular disease. The aim of the present study was to investigate the effect of six weeks of progressive aerobic exercise on expression of vascular endothelium growth factor (vegf) and tumor necrosis factor-alpha (tnfα) of the heart of diabetic male Wistar rats. Materials and Methods:  Forty male Wistar rats (weight 253.09 ± 12.92 grams, age 8-10 weeks) were randomly divided into four groups: control, diabetic, exercise, and diabetes + exercise. Induction of diabetes was done through intraperitoneal injection of streptozotocin solution at the rate of 50 mg/kg. The training protocol consisted of six weeks of progressive aerobic training, five sessions per week on the treadmill. The gene expression of vegf and tnfα in left ventricular tissue was measured by Real-Time PCR method. One-way analysis of variance and Tukey tests were used for statistical analysis at p>0.05 level. Results: The expression of the vegf and tnfα genes in the left ventricle of the heart in the diabetic group was significantly increased (p<0.0001) and decreased (p<0.0001) respectively compared to the healthy group. On the other hand, the expression of vegf and tnfα genes in the left ventricle of the diabetes + exercise group increased (p<0.003) and decreased respectively (p<0.0001) compared to the diabetes group. Conclusion: Progressive aerobic training can considered as a suitable non-pharmacological method for reduction of the inflammation and also to improve angiogenesis in diabetic samples.

کلیدواژه‌ها [English]

  • Diabetes
  • Aerobic training
  • Vascular endothelium growth factor
  • Tumor necrosis factor alpha
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