نوع مقاله : مقاله پژوهشی
نویسندگان
1 کارشناسی ارشد فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه صنعتی شاهرود، شاهرود، ایران.
2 دانشیار گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه صنعتی شاهرود، شاهرود، ایران.
3 استادیار گروه تربیت بدنی و علوم ورزشی، واحد شاهرود، دانشگاه آزاد اسلامی، شاهرود، ایران.
چکیده
زمینه و هدف: چاقی دوران کودکی در سراسر جهان در حال افزایش است و تاثیرات بی شماری بر سایر بافت‌های بدن از جمله تیروئید دارد. از این رو، پژوهش حاضر به بررسی تاثیر تمرین هوازی همراه با مکمل یاری سیر و استویا بر تغییرات هورمونی و هیستولوژیک بافت تیروئید رت های نر چاق تغذیه شده با رژیم غذایی پر چرب می‌پردازد. روش تحقیق: تعداد 35 سر رت نر نژاد ویستار به صورت تصادفی در هفت گروه کنترل سالم، القاء چاقی، القاء چاقی + سیر، القاء چاقی + استویا، القاء چاقی + تمرین هوازی، القاء چاقی + سیر + تمرین هوازی، و القاء چاقی + استویا + تمرین هوازی تقسیم شدند. تمرینات هوازی شامل 40 دقیقه در روز، با سرعت متغیر بین هشت تا پانزده متر در دقیقه و پنج روز در هفته، در طول هشت هفته اجرا گردید. عصاره سیر و استویا با غلظت 250 میلی گرم به ازای هر کیلوگرم وزن، نیز به آب مصرفی روزانه اضافه شد. بافت تیروئید جهت مطالعات ساختاری و پنج سی‌سی خون برای اندازه گیری سطح هورمون محرک تیروئید (TSH)، تری‌یدو‌تیرونین (T3) و تیروکسین (T4) پلاسما اخذ و به آزمایشگاه های مربوطه ارسال شدند. برای مقایسه گروه ها از آزمون تحلیل واریانس یک راهه و آزمون تعقیبی LSD در سطح معنی داری 05/0>p استفاده گردید. یافته ها: 12 هفته رژیم پرچرب در رت ها، موجب عدم تغییر سطوح سرمی هورمون TSH و T3 نسبت به گروه کنترل سالم شد (05/0<p). از طرف دیگر، میزان T4 سرمی رت‌های چاق نسبت به گروه کنترل سالم، به طور معنی دار افزایش یافت (05/0>p). از طرف دیگر، تحلیل بافتی نشان داد که چاقی موجب تغییرات ساختاری متوسط تا شدید بافت تیروئید می شود؛ اما مکمل یاری سیر، استویا و تمرین هوازی و ترکیب عصاره سیر - استویا - تمرین هوازی؛ موجب کاهش خفیف عوارض چاقی می گردند. نتیجه‌گیری: القاء رژیم پرچرب موجب تغییرات ساختاری قابل توجه در بخش فولیکولار، پارا-فولیکولار و کلوئید رت های نر ویستار می شود؛ اما ترکیب تمرین هوازی و مکمل یاری سیر و استویا، در بهبود این عوارض بصورت خفیف سودمند است.
کلیدواژه‌ها
عنوان مقاله [English]
The effect of aerobic exercise with garlic and stevia supplementation on hormonal and histological changes in thyroid tissue of obese rats fed a high-fat diet
نویسندگان [English]
- Mojtaba Hokmabadi 1
- Ali Younesian 2
- Seyed Javad Ziaolhagh 3
1 MS.c in Exercise Physiology, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Shahrood University of Technology, Shahrood, Iran.
2 Associated Professor at Exercise Physiology Department, Faculty of Physical Education and Sport Sciences, Shahrood University of Technology, Shahrood, Iran.
3 Assistant Professor at Physical Education & Sport Department, Shahrood Branch, Islamic Azad University, Shahrood, Iran.
چکیده [English]
Background and Aim: Childhood obesity has worldwide increasing and has numerous effects on other body tissues, including the thyroid. Therefore, the present study investigated the effect of aerobic exercise with garlic and stevia supplementation on hormonal and histological changes in thyroid tissue in obese rats fed with high-fat diet. Materials and Methods: Thirty five Wistar rats were randomly divided into seven groups of healthy control: obesity induction, obesity induction+garlic, obesity induction+stevia, obesity induction+aerobic exercise, obesity induction+garlic+aerobic exercise, and obesity induction+stevia+aerobic exercise. Aerobic exercises including 40 minutes training were performed eight to 15 m/min and five days per week, for eight weeks. Garlic and stevia extracts with a concentration of 250 mg/kg were added to the daily water intake. For structural studies the thyroid tissue and five cc of blood was transportd to the lab. for measuring the serum levels of thyroid stimulating hormone (TSH), triiodothyronine (T3) and thyroxine (T4). . For statistical analysis the one-way analysis of variance and LSD post hoc tests were used at the significant level of p<0.05. Results: Twelve weeks of high-fat diet in rats did not change the serum levels of TSH and T3 compared to the healthy control group (p<0.05). On the other hand, serum T4 levels of obese rats significantly increased compared to healthy controls (p<0.05). Histological analysis, on the other hand, showed that obesity causes moderate to severe structural changes in thyroid tissue; but garlic supplementation, stevia and aerobic exercise, and a combination of garlic-stevia extract and aerobic exercise slightly reduce the effects of obesity. Conclusion: Induction of high-fat diet causes significant structural changes in the follicular, par follicular and colloidal sections of male Wistar rats; but the combination of aerobic exercise and garlic and stevia supplementation is mildly helpful in improving these side effects.Keywords: Aerobic exercise, Stevia, Garlic, Obesity, Thyroid hormones.
کلیدواژه‌ها [English]
- Aerobic exercise
- Stevia
- Garlic
- Obesity
- Thyroid hormones
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