نوع مقاله : مقاله پژوهشی
نویسندگان
1 دکتری فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه فردوسی مشهد، ایران.
2 دانشیار گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه فردوسی مشهد، ایران.
3 استاد گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه فردوسی مشهد، ایران.
4 استادیار گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه فردوسی مشهد، ایران.
چکیده
زمینه و هدف: از آنجا که خستگی جسمی ناشی از انجام فعالیت ورزشی طاقت فرسا، بر ساختار و عملکرد سیستم ایمنی تاثیر منفی می گذارد؛ اتخاذ تدابیر تغذیه ای برای تعویق خستگی و ممانعت از افت عملکرد سیستم ایمنی، پیشنهاد می شود. به همین منظور در این پژوهش؛ اثر هشت هفته برنامه آماده سازی فوتبال و مصرف مکمل بتاولگاریس بر محتوای پروتئینهای OPA1 و MFN1 سلول های ایمنی تک هسته ای محیطی خون بازیکنان فوتبال مرد جوان، مورد مطالعه قرار گرفت. روش تحقیق: تعداد 30 بازیکن فوتبال مرد با میانگین سن 72/1±1/20 سال که در دو سال قبل از مطالعه به طور منظم در تمرینات و مسابقات شهرستان شیراز شرکت داشتند، به دو گروه تمرینی همراه با مصرف مکمل بتاولگاریس (15 نفر) و گروه تمرینی همراه با مصرف دارونما (15 نفر) تقسیم شدند و یک برنامه تمرین آمادهسازی فوتبال هشت هفته با تکرار سه جلسه در هفته شامل: تمرینات تناوبی، مقاومتی، سرعتی، پلایومتریک و بازی در گروه های کوچک را اجرا کردند. قبل از هر جلسه تمرین، ۵۰ گرم مکمل بتا ولگاریس حاوی ۱۳/۶ میلی مول نیترات حل شده در نیم لیتر آب، به هر بازیکن داده شد. قبل و بعد از مداخله، محتوای پروتئین های OPA1 و MFN1 سلولهای ایمنی تک هسته ای با روش الایزا اندازه گیری شد. دادهها با استفاده از آزمون تحلیل واریانس با اندازه گیری های تکراری در سطح معنی داری کمتر از 0/05 مورد بررسی قرار گرفتند. یافته ها: مقادیر پروتئین MFN1 در گروه تمرین- مکمل (0/001= p) و تمرین - دارونما (0/009=p) افزایش معنی داری داشت. مقادیر پروتئین OPA1 تنها در گروه تمرین - مکمل افزایش معنی دار داشت (0/001=p)، اما در گروه تمرین - دارونما معنی دار نبود (0/72=p). نتیجه گیری: به نظر می رسد تمرینات فوتبال و مصرف مکمل بتاولگاریس، هر کدام با مکانیزمی جداگانه، با افزایش محتوای پروتئین های OPA1 و MFN1 بتوانند از ایجاد اختلال در فرآیند بایوژنز میتوکندریایی جلوگیری کنند.
کلیدواژه‌ها
عنوان مقاله [English]
The effect of eight weeks of soccer preparation program and beta vulgaris supplementation on the content of OPA1 and MFN1 proteins in soccer players
نویسندگان [English]
- MohammadMehdi Maneshi 1
- Mehrdad Fathi 2
- Seyed Reza Attarzadeh Hosseini 3
- Mohammad Mosaferi Ziaaldini 4
1 PhD in Exercise Physiology, Faculty of Sports Sciences, Ferdowsi University of Mashhad, Iran.
2 Associate Professor at Department of Exercise Physiology, Faculty of Sports Sciences, Ferdowsi University of Mashhad, Iran.
3 Professor at Department of Exercise Physiology, Faculty of Sports Sciences, Ferdowsi University of Mashhad, Iran.
4 Assistant Professor at Department of Exercise Physiology, Faculty of Sports Sciences, Ferdowsi University of Mashhad, Iran.
چکیده [English]
Introduction and Aim: It is well-documented that physical fatigue from sports activities can negatively affect the immune system’s structure and function. Nutritional measures are suggested to delay fatigue and prevent the decline of immune system performance. The is study aimed to investigate the impact of an eight-week soccer preparation program and beta vulgaris supplement consumption on the content of OPA1 and MFN1 proteins in peripheral blood mononuclear immune cells of young male soccer players. Materials and Methods: The study involved thirty male soccer players with an average age of 20.1±1.72 y. who had regularly participated in training and competitions in Shiraz in the two years prior to the study. The participants were divided into two groups, with one group consuming beta vulgaris supplementation (n=15) and the other group receiving a placebo (n=15). The training program consisted of eight weeks of soccer preparation training, with three sessions per week. Before each training session, the players in the beta vulgaris group consumed 50 g of beta vulgaris supplement, which contained 6.13 mmol of nitrate dissolved in half a liter of water. The content of OPA1 and MFN1 proteins of mononuclear immune cells was measured before and after the intervention using the ELISA method. The data were analyzed using repeated measures of ANOVA test at p<0.05. Results: A study found that there was a significant increase in the MFN1 protein in both the exercise-supplement group (p=0.001) and the exercise-placebo group (p=0.009). Additionally, there was a significant increase in the OPA1 protein only in the exercise-supplement group (p=0.001), but not in the exercise-placebo group (p=0.72). Conclusion: The study concluded that soccer training and beta vulgaris supplement can prevent disruption of the mitochondrial biogenesis process by increasing the content of MFN1 and OPA1 proteins.
کلیدواژه‌ها [English]
- Soccer players
- Beta vulgaris supplement
- OPA1 protein
- MFN1 protein
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