نوع مقاله : مقاله پژوهشی
نویسندگان
1 استادیار گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه شهید چمران اهواز، اهواز، ایران.
2 دانشجوی دکتری بافت شناسی مقایسه ای، گروه علوم پایه، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران.
3 دکتری فیزیولوژی ورزشی، گروه تربیت بدنی و علوم ورزشی، دانشکده ادبیات و علوم انسانی، دانشگاه لرستان، لرستان، ایران.
4 استادیار گروه فیزیولوژی و فارماکولوژی، دانشکده پزشکی افضلی پور، مرکز تحقیقات فیزیولوژی و علوم اعصاب، پژوهشکده نوروفارماکولوژی، دانشگاه علوم پزشکی کرمان، کرمان، ایران.
چکیده
زمینه و هدف: بیماری دیابت موجب اختلال در هموستاز گلوکز و تغییرات منفی در عضله قلب می‌‌شود. از آنجا که فعالیت ورزشی ممکن است بافت قلبی افراد دیابتی را تحت تاثر قرار دهد، مطالعه حاضر با هدف بررسی اثر تمرین تناوبی با شدت بالا (HIIT) بر محتوای پروتئینهای FOXO3، PI3K و AKT در بافت قلبی موش‌های صحرایی نژاد ویستار مبتلا به دیابت نوع دو به اجرا درآمد. روش تحقیق: تعداد 36 سر موش صحرایی به چهار گروه شامل دیابت - کنترل، دیابت - تمرین، کنترل - سالم، و تمرین - سالم؛ تقسیم شدند. پس از دو ماه استفاده از رژیم غذایی پرچرب و القاء دیابت با استرپتوزوتوسین (35 میلی گرم به ازای هرکیلوگرم وزن بدن) در گروههای دیابت- کنترل و دیابت- تمرین؛ حیوانات در گروههای کنترل- سالم و تمرین- سالم پروتکلHIIT را بر اساس درصدی از Vmax به دست آمده و با تناوب های دو دقیقه ای و تعداد تناوب‌‌های فزآینده، به مدت هشت هفته با تکرار پنج جلسه در هفته، به اجرا درآوردند. زمان ۴۸ ساعت پس از آخرین جلسه تمرینی، بافت قلب استخراج شد و بررسی پروتئین‌‌‌ها با استفاده از روش وسترن بلات انجام گرفت. همچنین مطالعه هیستولوژیک در سطح بافتی با استفاده از رنگ آمیزی هماتوکسیلین و ائوزین انجام شد. از آزمون تحلیل واریانس یک راهه و آزمون تعقیبی توکی در سطح معنی داری 05/0>p برای تجزیه و تحلیل داده ها استفاده شد. یافته ها: محتوای پروتئین FOXO3 در گروههای دیابتی به صورت معنیدار نسبت به گروههای سالم، افزایش یافت (0/001=p). همچنین محتوای پروتئینهای PI3K و AKT در گروه دیابت - کنترل نسبت به گروه کنترل - سالم، به صورت معنی دار کاهش یافت (به ترتیب با 001/0=p و 0/009=p)، در حالی که محتوای این دو پروتئین، پس از تمرین افزایش معنیدار پیدا کرد (به ترتیب با 01/0= p و 0/001=p). در سطح بافت قلبی، ضخامت و طول میوسیت‌‌های قلبی بر اثر دیابت افزایش معنیدار نشان داد (0/001=p)؛ اما پس از HIIT، هایپرتروفی پاتولوژیک ایجاد شده به صورت معنیدار کاهش پیدا کرد (به ترتیب با 0/02=p و 0/01=p). نتیجه گیری: هر چند اجرای HIIT تغییری مبنی بر کاهش محتوای پروتئین FOXO3 ایجاد نکرد، توانست محتوای پروتئین های PI3K و AKT را افزایش داده و به طور مطلوب، هایپرتروفی پاتولوژیک ایجاد شده بر اثر دیابت را کنترل کند.
کلیدواژه‌ها
عنوان مقاله [English]
The effect of high intensity interval training on FOXO3, PI3K and AKT proteins content in heart muscle of type two diabetic rats
نویسندگان [English]
- Mohammad Rami 1
- Samane Rahdar 2
- Maryam Azimpour 3
- Kayvan Khoramipour 4
1 Assistant Professor at Department of Exercise Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
2 PhD Student in Comparative Histology, Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
3 PhD in Exercise Physiology, Department of Physical Education & Sport sciences, Faculty of Literature and Human Sciences, Lorestan University, Iran.
4 Assistant Professor at Department of Physiology and Pharmacology, Afzalipour Medical Faculty, Physiology Research Center and Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
چکیده [English]
Background and Aim: Diabetes can causes disturbances in glucose homeostasis and negative changes in the heart muscle. Since physical activity in people with diabetes may affect the heart tissue, the aim of this study was to investigate the effect of high intensity interval training (HIIT) on content of FOXO3, PI3K and AKT proteins in the heart tissue of Wistar rats with type two diabetes. Materials and Methods: In the present study, 36 male Wistar rats were divided into four groups: diabetes - control, diabetes - exercise, control – healthy, and exercise - healthy. After two months of high-fat diet and induction of diabetes by Streptozotocin (35 mg/kg) in diabetes-control and diabetes-exercise groups, the animals in diabetes-exercise and exercise-healthy groups performed the HIIT protocol based on a percentage of Vmax achieved, that it was with two-minutes intervals and increasing number of intervals for eight weeks and five sessions per week. 48 hours after the last training session, cardiac tissue was extracted and the content of FOXO3, PI3K and AKT proteins were assessed using Western blotting. In addition, a histological study was performed at the tissue level using hematoxylin and Eosin staining. To analyze the data one-way analysis of variance and Tukey tests were used at a significance level of p<0.05. Results: The content of FOXO3 protein in diabetic groups significantly increased compared to healthy groups (p=0.001). In addition, the content of PI3K and AKT proteins in diabetes-control group also significantly decreased compared to healthy groups (p=0.001 and p=0.009, respectively), while the content of these two proteins significantly increased after training (p=0.01 and p=0.001, respectively). Moreover, at the tissue level of heart, the thickness and length of cardiac myocytes significantly increased due to diabetes (p=0.001); while after HIIT, this pathological hypertrophy reduced (p=0.02 and p=0.01, respectively). Conclusion: Finally, it can be stated that although the use of this training method did not show a change in the amount of FOXO3 protein, but it was able to increase the amount of PI3K and AKT proteins and improve the pathological hypertrophy caused by diabetes.
کلیدواژه‌ها [English]
- High intensity interval training
- Type two diabetes
- FOXO3
- PI3K and AKT proteins
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