نوع مقاله : مقاله پژوهشی
نویسندگان
1 کارشناس ارشد فیزیولوژی ورزش، دانشکده علوم ورزشی، دانشگاه تربیت دبیر شهید رجایی، تهران، ایران.
2 استاد گروه فیزیولوژی ورزش، دانشکده علوم ورزشی، دانشگاه تربیت دبیر شهید رجایی، تهران، ایران.
3 دانشیار گروه فیزیولوژی ورزش، دانشکده علوم ورزشی، دانشگاه تربیت دبیر شهید رجایی، تهران، ایران.
چکیده
زمینه و هدف: اختلال در روند مرگ سلولی به شکل کاهش یا افزایش غیر طبیعی، منجر به بروز بیماری‌‌هایی از جمله، امراض قلبی - عروقی می‌‌شود. ورزش محرک فیزیولوژی قوی است که با تأثیر بر تعدادی از مسیرهای پیام دهی خارج و داخل سلولی، به طور مستقیم یا غیرمستقیم، بر روند آپوپتوزیس اثر می گذارد. هدف تحقیق حاضر بررسی اثر تمرین هوازی در آب بر بیان ژن‌‌های Apaf-1، سیتوکروم C و کاسپاز-9 بافت قلب در رت‌‌های تزریق شده با پراکسید هیدروژن بود. روش تحقیق: تعداد 24 سر رت نژاد ویستار با وزن تقریبی 10±190 گرم، به طور تصادفی ساده به چهار گروه شامل گروه‌‌های پراکسید هیدروژن، پراکسید هیدروژن + تمرین، شم و کنترل تقسیم شدند و طی شش هفته، هر هفته پنج روز، و به مدت 60 دقیقه در هر جلسه؛ به اجرای تمرین هوازی در آب وادار شدند. به همه گروه‌‌ها به غیر از گروه‌‌های کنترل و شم، به میزان یک میلی مول آب اکسیژنه به صورت درون صفاقی تزریق شد. زمان 48 ساعت بعد از آخرین جلسه و پس از 10 تا 12 ساعت ناشتایی، رت‌‌ها با گاز CO2 بیهوش شدند و بافت‌برداری از آن‌‌ها انجام شد. به منظور اندازه‌‌گیری بیان ژن ها از روش Real Time PCR و برای تجزیه و تحلیل داده‌‌ها، از آزمون تحلیل واریانس یک راهه و آزمون تعقیبی شفه در سطح معنی داری 0/05≥p استفاده شد. یافته‌‌ها: تزریق درون صفاقی پراکسید هیدروژن، بیان ژن‌‌های مسیر داخلی آپوپتوزی را نسبت به گروه شم و کنترل افزایش داد (0001/p=0)؛ در حالی که مداخله شش هفته ای تمرین هوازی در آب باعث کاهش معنی‌دار (0001/p=0) بیان ژن‌‌های Apaf-1 سیتوکروم C، و کاسپاز-9 در گروه پراکسید هیدروژن + تمرین نسبت به گروه پراکسید هیدروژن گردید. نتیجه‌گیری: تمرین هوازی در آب، کاهش تأثیرات نامطلوب آپوپتوزیس بافت قلب، آسیب کمتر سلولی و در نهایت، سلامتی بدن را به همراه دارد.
کلیدواژه‌ها
عنوان مقاله [English]
The effect of water aerobic exercise on the expression of Apaf-1, cytochrome C and Caspase-9 genes of cardiomyocyte tissue in male rats poisoned with hydrogen peroxide
نویسندگان [English]
- Sepideh Poursadeghi 1
- Majid Kashef 2
- Fereshteh Shahidi 3
1 MSc in Exercise Physiology, Faculty of Sport Sciences, Shahid Rajaee Teacher Training University, Tehran, Iran.
2 Professor of Department of Exercise Physiology, Faculty of Sport Sciences, Shahid Rajaee Teacher Training University, Tehran, Iran.
3 Associate Professor of Department of Exercise Physiology, Faculty of Sport Sciences, Shahid Rajaee Teacher Training University, Tehran, Iran.
چکیده [English]
Background and Aim: The cardiovascular disease is due to the some disorders in the cell death as the form of abnormal increased or decreased. Exercise is a strong physiological stimulus that directly or indirectly affects the process of apoptosis by means of a number of extracellular and intracellular signaling pathways. The aim of this study was to examine the effect of aerobic exercise in water on the genes expression of Apaf-1, cytochrome C and caspase-9 in heart tissue of rats which were injected with hydrogen peroxide. Materials and Methods: Twenty four male Wistar rats with an approximate weight of 190±10 grams were randomly divided into four groups including hydrogen peroxide, hydrogen peroxide+training, sham and control groups. The aerobic exercise protocol were performed with duration of 60 minutes in each session, six weeks and five days per week in water. All groups, except the control and sham groups, were injected intraperitoneally with one mill mole of hydrogen peroxide. Forty eight hours after the last training session with 10 to 12 hours of fasting, the rats were anesthetized with CO2 gas and their tissues were dissected. In order to measure genes expression using the Real Time-PCR method, one-way analysis of variance and Scheffe’s post hoc tests were applied for data analysis at significance level of p≤0.05. Results: The results indicated that intraperitoneal injection of hydrogen peroxide increased the genes expression of the internal apoptotic pathway compared to the sham and control groups (p=0.0001); while by adding six weeks of aerobic exercise in water, genes expression of Apaf-1, cytochrome C, and caspase-9 in the hydrogen peroxide+training group was significantly decreased compared to the hydrogen peroxide group (p=0001). Conclusion: It appears that aerobic exercise in water reduces the undesirable effects of apoptosis of heart tissue, less cytopathic and ultimately, it causes health of the body.
کلیدواژه‌ها [English]
- Water aerobic exercise
- Apoptosis
- Gene Expression
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