نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی دکتری فیزیولوژی ورزش، گروه علوم ورزشی، دانشگاه آزاد اسلامی، واحد بجنورد، بجنورد، ایران.
2 استادیار گروه علوم ورزشی، دانشگاه آزاد اسلامی، واحد بجنورد، بجنورد، ایران.
3 استادیار گروه علوم ورزشی، دانشگاه بجنورد، بجنورد، ایران.
چکیده
زمینه و هدف: اگرچه تمرینات ورزشی و آنتی‌‌اکسیدان‌‌ها سلامت مغز را بهبود می بخشند، اثر تعاملی تمرین مقاومتی و ژل رویال به خوبی مشخص نشده است. لذا هدف از مطالعه حاضر، بررسی اثر تمرینات مقاومتی با مکمل‌‌دهی ژل رویال بر بیان ژن عامل رشد عصب (NGF) و گیرنده تیروزین کیناز A (TrkA) در بافت هیپوکامپ موش‌‌های صحرایی نر آلزایمری شده بود. روش تحقیق: در این مطالعه تجربی، به 42 سر موش صحرایی نر نژاد اسپراگ ـ داولی، تری‌‌متیل‌‌تین (8 میلی گرم/کیلوگرم وزن بدن) تزریق شد. سپس موش ها به صورت تصادفی و به طور مساوی به 7 گروه شامل گروه کنترل، تمرین مقاومتی، تمرین مقاومتی+مصرف ژل رویال با دوز 100 میلی گرم/کیلوگرم، تمرین مقاومتی+مصرف ژل رویال با دوز 200 میلی گرم/کیلوگرم، مصرف ژل رویال با دوز 100 میلی گرم/کیلوگرم، مصرف ژل رویال با دوز 200 میلی گرم/کیلوگرم، و گروه شم تقسیم شدند. پروتکل تمرین مقاومتی به مدت 8 هفته، سه جلسه در هفته با شدت 30 تا 100 درصد وزن بدن موش ها انجام شد. بررسی بیان ژن‌‌ها با استفاده از روش Real-time PCR انجام شد و تمام پرایمرها توسط نرم افزار Allele IDv7.8 طراحی گردیدند. به منظور تجزیه و تحلیل داده‌‌ها، از روش تحلیل واریانس دو-راهه و آزمون تعقیبی بونفرونی در سطح معنی داری 05/0>p استفاده شد. یافته‌‌ها: پروتکل تمرین مقاومتی موجب افزایش بیان NGF گردید (001/0=p). مصرف ژل رویال (با دوز 100 و 200 میلی گرم/کیلوگرم)، تمرین مقاومتی+مصرف ژل رویال (با دوز 100 و 200 میلی گرم/کیلوگرم) موجب افزایش معنی‌‌دار بیان NGF و گیرنده TrkA شد (001/0=p). از طرف دیگر، اثر مکمل ژل رویال بر بیان NGF و گیرنده TrkA وابسته به دوز مصرفی بود؛ به گونه ای که اثر دوز 200 نسبت به دوز 100 میلی گرم/کیلوگرم؛ به طور معنی‌‌داری بیشتر بود (001/0=p). نتیجه‌‌گیری: هر دو مداخله تمرین مقاومتی و مصرف ژل رویال، به تنهایی و در ترکیب با همدیگر، موجب افزایش بیان نوروتروفین‌ها در هیپوکامپ موش‌‌های صحرایی مبتلا به آلزایمر می گردد؛ اما مصرف ژل رویال با دوز بالاتر، بهبودی بیشتری ایجاد می کند.
کلیدواژه‌ها
عنوان مقاله [English]
The effect of resistance trainings along with Royal jelly supplementation on gene expression of nerve growth factor and tyrosine kinase A receptor in the hippocampal tissue of Alzheimer’s male rats
نویسندگان [English]
- Amin Ashofteh 1
- Sadegh Cheragh-Birjandi 2
- Hossein TaheriChadorneshin 3
1 PhD Student of Exercise Physiology, Islamic Azad University, Bojnourd Branch, Bojnourd, Iran.
2 Assistant Professor, Department of Sport Sciences, Islamic Azad University, Bojnourd Branch, Bojnourd, Iran.
3 Assistant Professor, Department of Sport Sciences, University of Bojnord, Bojnourd, Iran.
چکیده [English]
Background and Aim: Although exercise training and antioxidants improve brain health, interactive effect of resistance training and Royal jelly has not yet been well established. Therefore, the aim of the current study was to investigate the effect of resistance training along with Royal jelly supplementation on hippocampal gene expression of nerve growth factor (NGF) and tyrosine kinase A (TrkA) receptor in rat model of Alzheimer’s disease. Materials and Methods: In this experimental study, 42 male Sprague-Dawley rats were injected by Trimethyltin (8 mg/kg/body weight). Then, the rats were randomly divided into 7 equal groups including control, resistance training, resistance training+100 mg/kg Royal jelly supplementation, resistance training+200 mg/kg Royal jelly supplementation, 100 mg/kg Royal jelly supplementation, 200 mg/kg Royal jelly supplementation and sham groups. The resistance training protocol was performed for 8 weeks, three sessions per week at intensity to 30-100% of their body weight. Gene expression was assessed using Real-Time PCR and all primers were designed by Allele IDv7.8 software. Data were analyzed using two-way analysis of variance and Bonferroni post hoc tests at the p<0.05. Results: The resistance training induced a significant increase in NGF expression (p= 0.001). Moreover, 100 and 200 mg/kg Royal jelly supplementation, resistance training+100 and 200 mg/kg Royal jelly supplementation resulted in a significant increases in expression of NGF and TrkA receptor (p=0.001). In addition, the effect of royal jelly supplementation on NGF and TrkA receptor expression was dependent on its dosage, where the dose of 200 mg/kg was significantly higher than the dose of 100 mg/kg (p=0.001). Conclusion: Both resistance training and Royal jelly supplementation, alone and synergistically, can increase neurotrophins expression in the hippocampus of Alzheimer’s rats; however higher dose of Royal jelly supplementation may induce more improvement.
کلیدواژه‌ها [English]
- Alzheimer’s disease
- Resistance training
- Tyrosine kinase A
- Nerve growth factor
- Royal jelly
DehBozorgi, A., Behboudi, L,. Hosseini, S.A., & Rasoli, M.H. (2020). Effect of voluntary and forced training with Royal jelly consumption on learning and spatial memory of rat model of alzheimer’s disease. Jundishapur Journal of Chronic Disease Care, 9(1), e97261. [Persian]
Dehbozorgi, A., Behbudi, Tabrizi, L., Hosseini, S.A., Haj Rasoli, M. (2020). Effects of swimming training and Royal jelly on BDNF and ngf gene expression in hippocampus tissue of rats with alzheimer’s disease. Zahedan Journal of Research in Medical Sciences, 22(2), e98310. [Persian]
e Silva, T.G.D.S., da Silva, J. R.M., da Silva Alves, A., Britto, L.R.G., Xavier, G F., & Sandoval, M.R. L. (2020). Oral treatment with Royal jelly improves memory and presents neuroprotective effects on icv-STZ rat model of sporadic Alzheimer’s disease. Heliyon, 6(2), e03281.
Giti, Z., Banaeifar, A., Arshadi, S., & Azarbayjani, M. A. (2020). Giti, Z., Banaeifar, A., Arshadi, S., & Azarbayjani, M. A. (2021). Effect of eight weeks of positive slope and negative slope training, along with Royal jelly on the hippocampal expression of β-amyloid and γ-secretase in trimethyltin-induced Alzheimer’s disease rats. Journal of Nutrition, Fasting and Health, 9(1), 29-34. [Persian]
Hall, J. M., Gomez-Pinilla, F., & Savage, L. M. (2018). Nerve growth factor is responsible for exercise-induced recovery of septohippocampal cholinergic structure and function. Frontiers in neuroscience, 12, 773.
Hassanlouei, F., Hoseini, S. A., Tabrizi, L.B., & Rasouli, M.H. (2020). The effect of endurance training with royal jelly consumption on dopamine in the hippocampus tissue of rats with Alzheimer’s disease. Food & Health, 3(1), 6-10. [Persian]
Hosseini, S.A., Salehi, O.R., Farzanegi, P., Farkhaie, F., Darvishpour, A.R., & Roozegar, S. (2020). Interactive effects of endurance training and royal jelly consumption on motor balance and pain threshold in animal model of the Alzheimer disease. Archives of Neuroscience, 7(2), 1-5. [Persian]
Jafarzadeh, G., Shakeryan, S., Farbood, Y., & Ghanbarzadeh, M. (2019). Effect of one session of resistance exercises on expression of BDNF gene and TrkA receptor in alzheimer model male Wistar rats. Journal of Fasa University of Medical Sciences, 8(4), 1176-1167. [Persian]
Jiang, G., Wang, C., Zhang, J., & Liu, H. (2017). Mediation of insulin growth factor-1 in alzheimer’s disease and the mechanism of PRNP genetic expression and the pi3k/akt signaling pathway. Experimental and Therapeutic Medicine, 13(6), 2763-2766.
Lippi, G., Mattiuzzi, C., & Sanchis-Gomar, F. (2020). Updated overview on interplay between physical exercise, neurotrophins, and cognitive function in humans. Journal of Sport and Health Science, 9(1), 74-81.
Mufson, E.J., Counts, S.E., Ginsberg, S.D., Mahady, L., Perez, S.E., Massa, S.M., ... & Ikonomovic, M.D. (2019). Nerve growth factor pathobiology during the progression of alzheimer’s disease. Frontiers in Neuroscience, 13, 533.
Negarande, Z., Salamat, K., Hosseini, S.A, & Etemad, Z. (2019). The effect of endurance training with crocin consumption on IGF-1 and glycogen expression in rat hippocampus tissue of trimethyltin-treated model of alzheimer’s disease. Asian Journal of Sports Medicine, 10(3), e92246. [Persian]
Omidi, M., Ghanbarzadeh, M., Nikbakht, M., Habibi, A., & Ranjbar, R. (2018). Effect of continuous aerobic exercise on nerve growth factor in diabetic rats. Health Scope, 9(1), 1-6. [Persian]
Özbeyli, D., Sarı, G., Özkan, N., Karademir, B., Yüksel, M., Kaya, Ö.T.Ç., & Çakır, Ö.K. (2017). Protective effects of different exercise modalities in an Alzheimer’s disease-like model. Behavioural Brain Research, 328, 159-177.
Salehi, O. R., Hoseini, A. (2017). The effects of endurance trainings on serum BDNF and insulin levels in streptozotocin-induced diabetic rats. Shefaye Khatam, 5 (2), 52-61.[Persian]
Shahed, A., Ravasi, A.A., Choubineh, S., & Khodadadi, D. (2018). Effect of four weeks exercise prior preparation before Alzheimer’s induction on the levels of nerve growth factor and beta Amyloid in the hippocampus of wistar male rats. Journal of Arak University of Medical Sciences, 20(11), 56-66. [Persian]
Uddin, M., Kabir, M., Jeandet, P., Mathew, B., Ashraf, G. M., Perveen, A., ... & Abdel-Daim, M.M. (2020). Novel anti-alzheimer’s therapeutic molecules targeting amyloid precursor protein processing. Oxidative Medicine and Cellular Longevity, 2020, 1-19.
Zamani, Z., Reisi, P., Alaei, H., Pilehvarian, A.A.(2012). Effect of Royal jelly on spatial learning and memory in rat model of streptozotocin-induced sporadic Alzheimer’s disease. Advanced Biomedical Research, 1(2), 1-6. [Persian]
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