نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی دکتری فیزیولوژی ورزشی، دانشکده علوم تربیتی و روانشناسی، دانشگاه محقق اردبیلی، اردبیل، ایران
2 استاد گروه تربیت‌‌بدنی و علوم ورزشی، دانشکده علوم تربیتی و روانشناسی، دانشگاه محقق اردبیلی، اردبیل، ایران.
3 استادیار بیوشیمی بالینی، مرکز تحقیقات علوم اعصاب، دانشکده پزشکی، دانشگاه علوم پزشکی وخدمات بهداشتی درمانی تبریز، تبریز، ایران.
4 استادیار گروه تربیت بدنی و علوم ورزشی، دانشکده علوم پایه، دانشگاه علوم انتظامی امین، تهران، ایران.
5 استادیار گروه تربیت‌‌بدنی و علوم ورزشی، دانشکده علوم تربیتی و روانشناسی، دانشگاه محقق اردبیلی، اردبیل، ایران.
چکیده
زمینه و هدف: آپوپتوزیس در بافت پس‌‌میتوزی همچون قلب که قابلیت تکثیر سلولی ندارد، مهلک است؛ چرا که از دست رفتن کاردیومیوسیت‌‌ها جایگزین شدنی نیست. این در حالی است که تمرین ورزشی می‌‌تواند در کاهش آپوپتوزیس مؤثر باشد. هدف مطالعه حاضر تاثیر 3 ماه تمرین هوازی منظم بر بعضی عوامل مسیر میتوکندریایی آپوپتوزیس در بافت قلبی موشهای نر ویستار بود. روش تحقیق: این تحقیق از نوع مطالعات کاربردی است که در قالب یک طرح تجربی با گروه کنترل به اجرا درآمد. تعداد 31 سر موش نر ویستار با میانگین وزنی 21/9± 80/173 گرم در سه گروه همگن کنترل سه ماهه (10 سر)، کنترل شش ماهه (10 سر) و تمرین هوازی (11 سر) تقسیم شدند. گروه تمرین هوازی به مدت 3 ماه در تمرینات ورزشی با شدت 75 الی 80 درصد حداکثر اکسیژن مصرفی شرکت کردند. پس از اجرای پروتکل تمرینی، مراحل جراحی و استخراج بافت قلبی انجام شد و بیان ژن‌‌های Bax و Bcl-2 با استفاده از روش RT-PCR ارزیابی گردید. نتایج با استفاده از آزمون تحلیل واریانس یک راهه و آزمون تعقیبی بونفرونی در سطح معنی‌‌داری 05/0>p استخراج گردید. یافته‌‌ها: بیان ژن Bcl-2 در گروه تمرین هوازی افزایش معنی‌‌دای در مقایسه با گروه کنترل شش‌‌ماهه پیدا کرد (001/0=p)؛ در حالی که بیان ژن Bax در گروه تمرین هوازی تغییر معنی‌‌داری در مقایسه با گروه کنترل شش‌‌ماهه نداشت (21/0=p). نتیجه‌‌گیری:تمرینات هوازی منظم می توانند در کنترل آپوپتوزیس میتوکندریایی بافت قلبی نقش داشته باشند.
کلیدواژه‌ها
عنوان مقاله [English]
The role of 3 months of regular aerobic training on some factors of mitochondrial apoptosis pathway in cardiac tissue of male Wistar rats
نویسندگان [English]
- Aylar Imani 1
- Marefat Siahkohian 2
- pouran karimi 3
- Masoud Asgharpour-Arshad 4
- Farnaz Seifi 5
1 Ph.D student of Exercise Physiology , Faculty of Educational Sciences and Psychology, Mohaghegh Ardabili University, Ardabil, Iran
2 Full Professor, Department of Physical Education and Sport Sciences, Faculty of Educational Sciences and Psychology, Mohaghegh Ardabili University, Ardabil, Iran.
3 Assistant Professor, Department of Clinical Biochemistry, Neuroscience Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Assistant Professor, Department of Physical Education and Sport Sciences, Faculty of Basic Sciences, Amin Police University, Tehran, Iran.
5 Assistant Professor, Department of Physical Education and Sport Sciences, Faculty of Educational Sciences and Psychology, Mohaghegh Ardabili University, Ardabil, Iran.
چکیده [English]
Background and Aim: Apoptosis in post-mitotic tissue such as the heart is fatal due to the unable to proliferate and loss of cardiomyocytes is irreplaceable. However, exercise training could be effective in reducing apoptosis. The aim of this study was to investigate the role of 3 months of regular aerobic training on some factors of mitochondrial apoptosis pathway in cardiac tissue of male Wistar rats. Materials and Methods: The research was applied study that conducted in the form of an experimental project with a control group. Thirty one male Wistar rats with an average weight of 173.80 ± 9.21 grams were divided into three homogeneous groups including three-months control (n=10), six-months control (n=10) and aerobic training group (n=11).The aerobic training group participated in aerobic training with 75 to 80 percent of maximal oxygen consumption for 3 months. After the implementation of the training protocol, the surgical procedures and cardiac tissue extraction were performed and gene expression of Bax and Bcl-2 of cardiac tissue was assessed by RT-PCR. Results extracted by one-way of variance analysis and Bonferroni post hoc test at a significance level of p<0.05. Results: The results showed the significant increase in Bcl-2 gene expression during aerobic training compared to the six-month control group (p=0.001). However, the expression of Bax gene in the aerobic training group did not show any significant changes compared to the control group (p=0.21). Conclusion: Regular aerobic exercise can play a significant role for controlling mitochondrial apoptosis of cardiac tissue.
کلیدواژه‌ها [English]
- Aerobic training
- Gene expression of Bcl-2
- Gene expression of Bax
- Myocard tissue
- Rat
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