نوع مقاله : مقاله پژوهشی
نویسندگان
1 استادیار گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران.
2 استادیار گروه تربیت بدنی، دانشگاه صنعتی شریف، تهران، ایران.
3 مربی گروه تربیت بدنی و علوم ورزشی، واحد سبزوار، دانشگاه آزاد اسلامی، سبزوار، ایران.
چکیده
زمینه و هدف: ورزش می تواند منجر به عصب‌زایی در مغز پستانداران بالغ شود. تاکنون مطالعات اندکی چگونگی تاثیر نوع ورزش بر عصب‌زایی را بررسی کرده‌اند. هدف مطتاعه حاضر بررسی تاثیر مسافت دویدن در روز بر میزان تکثیر سلولی در هیپوکمپ موش‌های صحرایی نر بالغ بود. روش تحقیق: تعداد 18 سر موش صحرایی نر بالغ به دنبال یک هفته آشنا سازی با دستگاه نوارگردان، به طور تصادفی به سه گروه شامل گروه کنترل (6 سر)، گروه دویدن به مدت 30 دقیقه (6 سر)، و گروه دویدن به مدت 60 دقیقه (6 سر) تقسیم شدند. حیوانات گروه های تجربی روزانه در جلسات 30 و 60 دقیقه ای با سرعت 12 متر بر دقیقه روی نوارگردان برای 14 روز متوالی دویدند. در 10 روز پایانی، به منظور نشاندار کردن سلول‌های پس از تقسیم، دوز مشخصی از برمو دی اکسی یوریدین (Brdu) به طور روزانه به حیوانات تزریق شد. 48 ساعت پس از آخرین جلسه فعالیت، حیوانات قربانی شدند و مغز آن‌ها جهت انجام مطالعات ایمونوهیستوشیمی برداشته شد. جهت تجزیه تحلیل داده‌ها از آزمون های تحلیل واریانس یک سویه و آزمون تعقیبی حداقل اختلاف معنی دار (LSD) در سطح معنی داری 05/0 >p استفاده شد. یافته‌ها: تعداد سلول‌های Brdu+ هم پس از دویدن به مدت 30 (001/p=) و هم پس از دویدن به مدت 60 (001/0p=) دقیقه افزایش معنی داری پیدا کرد؛ ضمن آن که این تغییر در گروه دویدن به مدت 60 دقیقه نسبت به گروه دویدن به مدت 30 دقیقه، به طور معنی دار (001/p=) بیشتر بود. نتیجه گیری: دویدن صرف نظر از زمان طی شده در روز، تکثیر سلولی را در هیپوکمپ موش‌های صحرایی نر بالغ افزایش می دهد؛ این در حالی است که هر چه زمان بیشتر در روز طی شود، میزان تکثیر سلولی بهمان نسبت بیشتر تغییر می کند.
کلیدواژه‌ها
عنوان مقاله [English]
Effect of running time on cell proliferation in the hippocampus of male adult rats
نویسندگان [English]
- Shima Mojtahedi 1
- Arezu Tbrizi 2
- Seyed Ebrahim Hosseini 3
1 Assistant Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran.
2 Assistant Professor, Department of Physical Education, Sharif University of Technology, Tehran, Iran.
3 Instructor of Department of Physical Education and Sport Sciences, Sabzevar Branch, Islamic Azad University, Sabzevar, Iran.
چکیده [English]
Background and Aim: Exercise can causes neurogenesis in the brain of adult mammals. Until now few studies investigated how the effect of exercise on neurogenesis. The aim of the present study was examine the effect of the running time on cell proliferation in the hippocampus of adult male rats. Materials and Methods: Eighteen adult male rats following one week of familiarization with treadmill were randomly divided into three groups including of control group (n=6), 30-min running group (n=6) and 60-min running group (n=6). The animals in running groups were subjected to daily 30-min and 60-min treadmill exercise sessions with velocity of 12 meter per minute for 14 consecutive days. At the last 10 days, animals received daily injections of bromodeoxyuridine (BrdU) in a specific dose to label dividing cells. After 48 hours of the last session of running, the animals were sacrificed and their brain was removed for immunohistochemichal analysis. The one-way analysis of variance followed by least significant difference (LSD) post hoc test was used to analyze the data at the significant level of p<0.05. Results: The number of Brdu+ cells increased significantly both after running for 30 (p=0.001) and 60 (p=0.001) minutes; so that these changes were significantly higher after the 60-min than to the 30-min running (p=0.001). Conclusion: The results of this study showed that running regardless of time traveled per day increases cell proliferation in the hippocampus of adult male rats; so that the longer the time, the greater the rate of cell proliferation.
کلیدواژه‌ها [English]
- Exercise training
- Hippocampus
- Cell proliferation
- Adult rats
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