نوع مقاله : مقاله پژوهشی
نویسندگان
گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران.
چکیده
زمینه و هدف: آلزایمر یک بیماری پیشرونده اختلال عصبی می‌‌باشد. التهاب نقش مهمی در اختلال عملکرد عصبی و از دست دادن سلول‌‌های عصبی در بیماری آلزایمر ایفا می‌‌کند. مطالعات قبلی نشان داده‌‌اند که فعالیت پروتئین متصل شونده به عنصر پاسخگوی cAMP (CREB) با بتا آمیلوئید و التهاب کاهش می‌‌یابد. بنابراین هدف از پژوهش حاضر، بررسی اثر تمرین هوازی قبل و بعد از القای آلزایمر بر بیان اینترلوکین-1 بتا (IL-1β) و CREB هیپوکمپ موش های نر نژاد ویستار بود. روش تحقیق: تعداد 115 سر رت نر بالغ (8 هفته) با میانگین وزنی 20/17±250 گرم در مرحله قبل از القای آلزایمر به صورت تصادفی به دو گروه مساوی استراحت (55 سر) و تمرین (55 سر) تقسیم شدند. پس از چهار هفته بعد از القای آلزایمر، رت‌‌های هر کدام از گروه‌‌ها به سه زیرگروه شامل گروه تزریق آمیلوئیدبتا؛ گروه تزریق دارونما؛ و گروه بدون تزریق تقسیم شدند. قبل از شروع مطالعه، پس از چهار هفته تمرین (قبل و بعد از القای آلزایمر)، حیوانات کشته شده (5 سر در هر گروه) و هیپوکمپ آن ها جهت بررسی برداشته شد. بیان IL-1β و CREB با روش Real Time-PCR اندازه‌‌گیری و داده‌‌ها در دو مرحله پیش و پس از القای آلزایمر، با آزمون تحلیل واریانس یک طرفه و آزمون تعقیبی حداقل احتلاف معنی دار (LSD) در سطح 05/0≥p تحلیل شدند. یافته‌‌ها: هم در مرحله قبل و هم بعد از القای آلزایمر، بیان IL-1β و CREB در گروه تمرین نسبت به گروه استراحت، به ترتیب کاهش و افزایش معنی داری پیدا کرد (05/0>p). نتیجه‌‌گیری: تمرین هوازی می تواند قبل و بعد از القای آلزایمر، منجر به به افزایش CREB و کاهش التهاب شده و احتمالاً از این طریق، به شکل پذیری هیپوکامپی کمک نموده و فوایـد شـناختی و عملکـردی در پی دارد.
کلیدواژه‌ها
عنوان مقاله [English]
Effect of aerobic training before and after induction of Alzheimer on interleukin-1ß and CREB gene expression in the hippocampus of Wistar male rats
نویسندگان [English]
- Mostafa Sabouri
- Mohammad Reza Kordi
- Fatemeh Shabkhiz
Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran.
چکیده [English]
Background and Aim: Alzheimer’s disease is a progressive disorder of the nervous system. Inflammation plays an important role in neurological dysfunction and loss of neuronal cells in Alzheimer’s disease. Previous studies have shown that cAMP response element-binding protein (CREB) activity decreases with beta amyloid and inflammation. Therefore, the purpose of this study was to investigate the effect of aerobic training before and after induction of Alzheimer on interleukin-1β (IL-1β) and CREB gene expression of Wistar male rats. Materials and Methods: The number of 115 male adult rats (eight-week old) with an average weight of 250±17.20 grams in the pre-Alzheimer’s stage were randomly divided into two equal resting (55 heads) and exercise (55 heads) groups. After four weeks and after induction of Alzheimer’s disease, the rats of each group were divided into three subgroups including Amyloid injection, injecting the placebo, and without injection groups. Before the start of the study, animals were killed (5 heads in each group) and their hippocampus removed after four weeks of training (before and after Alzheimer’s induction). IL-1β and CREB expression were measured by Real Time-PCR method and data were analyzed in two stages before and after Alzheimer’s induction with one way- analysis of variance and least significant difference (LSD) tests at the significant level of p≤0.05. Results: Both in the pre and post-Alzheimer’s stages, IL-1β and CREB expression decreased and increased significantly (p<0.05) in the training group compared to the resting group, respectively. Conclusion: Aerobic exercise can increase of CREB expression and reduction of inflammation before and after Alzheimer’s induction, and it probably contributes to hippocampal plasticity in this way and has cognitive or functional benefits.
کلیدواژه‌ها [English]
- Alzheimer
- cAMP response element-binding protein
- Interleukin 1 beta
- Exercise training
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