Background and Aim: The use of exercise training to correct metabolic disorders and treat diseases has become widespread. The aim of the present study was to investigate the effect of eight weeks of resistance and endurance training on AMPK and AXIN1 gene expression in adipose tissue of obese male diabetic mice. Materials and Methods: Twenty-four healthy male Wistar rats with an average age of eight weeks and an average weight of 187.5 ± 9.37 g were fed a high-fat diet for eight weeks. Eighteen of these animals were induced to have diabetes after eight weeks of high-fat diet. Intraperitoneal injection of streptozotocin was used to induce type 2 diabetes. Diabetic male rats were divided into three groups of six including diabetic control (DC), diabetic resistance training (RT), and diabetic endurance training (ET). Resistance training (at an intensity of 50 to 120 percent of body weight) and endurance training (with an intensity of 50 to 70% of maximum speed) were performed for eight weeks, five days a week. Real Time-PCR was used to measure the expression of AMPK and AXIN1 genes in adipose tissue. One-way analysis of variance and Tukey's post hoc test were used to determine the difference between groups at a significance level of p<0.05. Results: The results showed that there was a significant difference between the groups of the present study in terms of AMPK and AXIN1 gene expression in adipose tissue of male mice (p=0.001). Using Tukey's post hoc test, there was a significant decrease in AMPK and AXIN1 gene expression in adipose tissue of male mice in the DC group compared to the OC group (p=0.001). A significant increase in the expression of the aforementioned genes was observed in the ET and RT groups compared to the DC group (p=0.001). Also, no significant difference was observed between the training groups (p=0.12). Conclusion: Endurance and resistance exercise training (with similar effects) resulted in upregulation of AXIN1 and AMPK gene expression and can be considered as an effective strategy for reducing disorders related to overweight and obesity and type 2 diabetes.