نوع مقاله : مقاله پژوهشی
عنوان مقاله English
نویسندگان English
Background and Purpose: Obesity and high-fat diet consumption are associated with chronic low-grade inflammation, metabolic disorders, and an increased risk of metabolic diseases. Therefore, the present study aimed to investigate the effects of 10 weeks of high-intensity interval training (HIIT), with and without a high-fat diet, on body weight, fasting blood glucose, and protein expression of MCP-1 and NF-κB in obese male C57BL/6 mice. Methods: In this experimental study, 40 mice were randomly assigned into چهار groups: normal diet control (Ctrl/ND), exercise with normal diet (Ex/ND), sedentary with high-fat diet (Sedentary/HFD), and exercise with high-fat diet (Ex/HFD). The HIIT protocol was performed for 10 weeks, 5 sessions per week, on a treadmill. At the end of the intervention, body weight and fasting blood glucose were measured, and protein expression levels of MCP-1 and NF-κB were assessed using the Western blot technique. Results: Metabolic findings demonstrated that the high-fat diet significantly increased body weight and fasting blood glucose (P<0.05). In addition, protein expression levels of NF-κB and MCP-1 in the Sedentary/HFD group increased by 150% compared with the control group (P<0.05). In contrast, HIIT significantly attenuated the adverse effects of the high-fat diet. In the Ex/HFD group, body weight, fasting blood glucose, NF-κB protein expression, and MCP-1 protein expression decreased by approximately 8.3%, 25%, 36%, and 52%, respectively, compared with the Sedentary/HFD group (P<0.05). Conclusion: Overall, the findings suggest that HIIT can partially attenuate the detrimental effects of a high-fat diet on metabolic and inflammatory markers. However, the persistence of some inflammatory alterations in the high-fat diet group despite exercise indicates that dietary modification alongside physical activity is essential for effective inflammation control and prevention of metabolic disorders.
کلیدواژهها English
