نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی دکتری فیزیولوژی ورزشی، گروه علوم ورزشی، دانشگاه آزاد اسلامی، بجنورد، ایران.
2 دانشیار فیزیولوژی ورزشی، گروه علوم ورزشی، دانشگاه آزاد اسلامی، بجنورد، ایران.
3 استادیار فیزیولوژی ورزشی، گروه علوم ورزشی، دانشگاه آزاد اسلامی، بجنورد، ایران.
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Background and aim: Spinal cord injury (SCI) induces skeletal muscle atrophy, which is associated with impaired in PI3K and AKT pathways, whereas exercise training and antioxidant nutrients may inhibit muscle atrophy. The aim of the present study was to investigate the effect of four weeks of resveratrol supplementation, aerobic training, or their combination on muscle PI3K and AKT protein levels in rats with SCI injury. Materials and Methods: Forty rats were randomly divided into five groups (n=8) including healthy control group, control group (SCI), resveratrol group (RES), exercise training group (EX), and resveratrol and exercise combination group. The interventions commenced 14 days after injury induction and continued for four weeks. The aerobic training lasted 58 minutes at an intensity of 20 m/min for 4 weeks. Resveratrol supplementation was administered at a dose of 10 mg via intraperitoneal injection. After 4 weeks, the protein levels of PI3K and AKT in skeletal muscle were measured using the ELISA method. For intergroup comparison, one-way ANOVA test and Tukey's post hoc test were used at the p < 0.05 level. Findings: Exercise training, supplementation, and the combination of supplementation and exercise training significantly increased PI3K protein compared to the control group in rats with spinal cord injury (p<0.001). Also, this increase was significantly greater in the exercise + supplementation group compared to the supplementation and exercise training alone groups (p=0.006, =0.01, respectively). Resveratrol supplementation (p=0.01), exercise training (p=0.002), and their combination (p=0.001) significantly increased AKT protein compared to the control group in rats with spinal cord injury. This increase was significantly greater in the combination group compared to the supplementation and exercise training alone groups (p=0.006 & p=0.035, respectively). Conclusion: These results indicate that resveratrol and aerobic training restore the AKT/PI3K pathway induced by SCI. Their combined application is a superior therapeutic strategy to enhance this pathway in combating metabolic dysfunction and muscle atrophy induced by SCI.
کلیدواژهها [English]
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