نوع مقاله : مقاله پژوهشی
نویسندگان
1 کارشناسی ارشد فیزیولوژی ورزشی، واحد لارستان، دانشگاه آزاد اسلامی، لارستان، ایران.
2 دانشیار گروه فیزیولوژی ورزشی، واحد مرودشت، دانشگاه آزاد اسلامی، مرودشت، ایران.
3 استادیار گروه فیزیولوژی ورزشی، واحد گیلان غرب، دانشگاه آزاد اسلامی، گیلان غرب، ایران.
چکیده
زمینه و هدف: نقش مطلوب ورزش و مکمل های آنتی اکسیدانی در بافت کلیه گزارش شده است؛ اما تاثیر تعاملی آن ها هنوز به خوبی شناخته نشده است. این پژوهش اثر هشت هفته تمرینات مقاومتی همراه با مکمل روی بر عامل نکروز دهنده تومور آلفا (TNF- α) و پروتئین واکنشگر C (CRP) بافت کلیه در موش های صحرایی متعاقب انسداد یک طرفه کامل حالب را بررسی می کند. روش تحقیق: در این مطالعه تجربی، 48 سر موش صحرایی نر نژاد اسپراگو - داولی با محدوده سنی 12-16 هفته و دامنه وزنی 250-220 گرم که حالب آن ها به طور کامل طی جراحی مسدود شده بود، به طور تصادفی در گروه های کنترل نفرکتومی، مصرف مکمل روی، تمرین مقاومتی و تمرین مقاومتی+ مکمل روی؛ تقسیم شدند. تعداد هشت سر موش صحرایی سالم هم برای بررسی اثرات نفرکتومی، به عنوان گروه کنترل سالم و هشت سر موش که عمل جراحی بدون آسیب به کلیه در آن ها انجام شد، به عنوان گروه شم (Sham) در نظر گرفته شدند. گروه های تمرین مقاومتی به مدت هشت هفته، سه جلسه در هفته و با شدت 30 تا 100 درصد وزن بدن؛ به تمرین پرداختند. گروه های مصرف مکمل، روزانه 30 میلی گرم/کیلوگرم وزن بدن، روی را به صورت خوراکی دریافت کردند. برای تجزیه و تحلیل داده ها از آزمون تحلیل واریانس یک راهه همراه با آزمون تعقیبی توکی در سطح معنی داری 05/0>p. استفاده شد. یافته ها: نتایج نشان داد که TNF-α و CRP در گروه های تمرین و مصرف مکمل، به طور معنی داری کمتر از گروه های عدم تمرین (کنترل) است (001/0= p). همچنین اثر توام تمرین و مکمل روی موجب کاهش معنی دار CRP در موش های صحرایی متعاقب انسداد یک طرفه حالب شد (001/0=p). نتیجه‌‌گیری: به نظر می رسد تمرین مقاومتی و مکمل روی، هم به تنهایی و هم به طور توام، دارای اثرات سینرژیستی بر کاهش عوامل التهابی کلیه می باشند.
کلیدواژه‌ها
عنوان مقاله [English]
The effect of resistance training and zinc supplementation on TNF-α and CRP in the kidney tissue of rats following complete unilateral ureteral obstruction
نویسندگان [English]
- Hossain Jokar 1
- Sirous Farsi 2
- Mehran Ghahramani 3
1 MSc in Exercise Physiology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran.
2 Associate Professor at Department of Exercise Physiology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran.
3 Assistant Professor at Department of Exercise Physiology, Gilan-e-Gharb Branch, Islamic Azad University, Gilan-e-Gharb, Iran.
چکیده [English]
Background and Aim: Role of exercise training and antioxidant supplements has been reported in the kidney tissue, but their interactive effect is still not well understood. This research examines the effect of eight weeks of resistance training and zinc supplementation on tumor necrosis factor alpha (TNF-α) and C -reactive protein (CRP) of kidney tissue in rats following complete unilateral ureteral obstruction. Materials and Methods: In this experimental study, 32 male Sprague-Dawley rats with an age range of 12-16 weeks and a weight range of 220-250 grams were selected; the rats ureters were completely obstructed during surgery, were randomly divided into four groups including: nephrectomy control, Zinc supplementation, resistance training, and resistance training + Zinc supplementation. Moreover, to investigate the effects of nephrectomy, eight healthy rats were assigned in the healthy control group and eight one that underwent surgery with no kidney obstruction were assigned in the sham group. The resistance training groups performed climbing with an intensity of 30-100% of their body weight three times a week for eight weeks. The Zinc supplementation groups received 30 mg/kg/w Zinc supplement orally each day. To analyze the data, one-way analysis of variance with Tukey’s post hoc test were used at p<0.05 level. Results: The results showed significant reduction in TNF-α and CRP levels in both training and supplementation groups than the non-training (control) groups (p=0.001). Also, the interaction of training and supplementation was significant in reducing CRP levels in rats following unilateral ureteral obstruction (p=0.001). Conclusion: It appears that resistance training and Zinc supplementation both individually and interactively, have synergistic effects on reducing inflammatory factors; however, further studies are needed about the interactive effect of these factors on TNF-α levels.
کلیدواژه‌ها [English]
- Resistance training
- Zinc supplement
- Tumor necrosis factor alpha
- C-reactive protein
- Nephrectomy
Abedi Kushalshahi, A., Azarnia, M., Jamali, M., & Moir, F. (2013). Investigating the effect of culture medium obtained from human mesenchymal stem cells on acute kidney injury caused by gentamicin in rats. Cell and Tissue, 5(1), 39-52. [In Persian]. https://doi.org/10.52547/JCT.5.1.39.
Azamian Jazi, A., Sarteshnizi, E.M., & Fathi, M. (2021). Effect of selected elastic band resistance training on serum levels of fibroblast growth factor 23, TNF-α and hsCRP in overweight elderly women. The Iranian Journal of Obstetrics, Gynecology and Infertility, 24(1), 26-35. [In Persian]. https://doi.org/10.22038/ijogi.2021.17990.
Bozalioglu, S., Ozkan, Y., Turan, M., & Simsek, B. (2005).Prevalence of zinc deficiency and immune response in short-term hemodialysis. Journal of Trace Elements in Medicine and Biology, 18(3), 243-9. https://doi.org/10.1186/s13102-022-00571-6
Brown, K. (1998). Effect of infections on plasma zinc concentration and implications for zinc status assessment in low-income countries. American Journal of Clinical Nutrition, 68(supp l), 425–429. https://doi.org/10.1093/ajcn/68.2.425S.
Coelho, P., Diniz, AD., & Arruda, I. (2005). Vitamin A and zinc status in patients on maintenance haemodialysis. Nephrology, 10(5), 459-63. https://doi.org/10.1111/j.1440-1797.2005.00469.x. PMID: 16221095.
Gholipour Baradari, A., Ëmami Zeydi, A., Khademlu, M., Naghshvar, F., Razavi Baladehi, M., & Ëspahbodi ,F.(2011). The effect of intravenous vitamin Ç on the level of ÇRP in hemodialysis patients. Journal of Mazandaran University of Medical Sciences, 20(80), 55-61. [In Persian]. URL:http://jmums.mazums.ac.ir/article-1-647-fa.html.
Heidarianpour, A., & Keshvari, M. (2016). Effects of three types of exercise aerobic, resistance and concurrent on plasma CRP concentration in type II diabetes patients. Journal of Sabzevar University of Medical Sciences, 23(6), 916-925. [In Persian]. URL: http://jsums.medsab.ac.ir/article_937.html.
Kabir, B., Taghian, F., & Ghatreh Samani, K. (2018). Dose 12 week resistance training influence IL-18 and CRP levels in elderly men? Razi Journal of Medical Sciences, 24(165), 77-84. [In Persian]. URL: http://rjms.iums.ac.ir/article-1-3201-fa. Ht ml.
Klahr, S., & Morrissey, J. (2012). Obstructive nephropathy and renal fibrosis. American Journal of Physiology-Renal Physiology, 283(5), F861-875. https://doi.org/10.1152/ajprenal.00362.
Li, A., Liang, L., Liang, P,. HU, Y., XU, C., HU, X., & Kamal,I . (2020). Assessment of renal fibrosis in a rat model of unilateral ureteral obstruction with diffusion kurtosis imaging: Comparison with α-SMA expression and 18F-FDG PET. Magnetic Resonance Imaging, 66, 176-184. https://doi.org/10.1016/j.mri.2019.08.035
Mazaheri, B., Emami, F., Moslemi, F., Talebi, A., & Nematbakhsh, M. (2019). Zinc Supplementation and Ischemia Pre-conditioning in Renal Ischemia/Reperfusion Injury. The Malaysian Journal of Medical Sciences, 26(4), 39-46. https://doi.org/10.21315/mjms2019.26.4.5. Epub 2019 Aug 29.
Mehrabi, M., Kazemzadeh, Y., Gorzi, A., Hosseini. S.A., & Sedaghati, S. (2023). Effect of eight weeks of resistance training on inflammatory markers and antioxidant indices of kidney tissue following Testosterone Enanthate abuse in male rats. Journal of Shahid Sadoghi University, 31(7), 6873-6884. [In Persian]. https://doi.org/10.18502/ssu.v31i7.13698.
Moraes, M.R., Rosa, T.S., Souza, M.K., Neves, R.V.P., Bacurau, R.F.P., Passos, CS., & Câmara, N.O.S. (2018). Resistance training downregulates macrophages infiltration in the kidney of 5/6 nephrectomized rats. Life Sciences, 213, 190-197. https://doi.org/10.1016/j.lfs.2018.10.037.
Peake, J.M., Kukuljan, S., Nowson, C.A, Sanders, K., & Daly, R.M. (2011). Inflammatory cytokine responses to progressive resistance training and supplementation with fortified milk in men aged 50+ years: an 18- month randomized controlled trial. European Journal of Applied Physiology, 111(12), 3079-88. https://doi.org/10.1007/s00421-011-1942-z.
Peter, D., Ai Q T.T., Dion, G., Lat,a J., Chiara, M., & Richard, E.R. (2005). Zinc metabolism in airway epithelium and airway inflammation: basic mechanisms and clinical targets. A review. Pharmacology & Therapeutics Journal, 105(2), 127-49. https://doi.org/10.1016/j.2004.09.004.
Rashidi, A.A., Salehi, M., & Sagheb, M.A. (2007). Effect of Zinc supplement on CRP inflammatory marker in hemodialysis patients. Feyz Medical Sciences Journal, 11(2), 29-33. [In Persian]. URL: http://feyz.kaums.ac.ir/article-1-36-fa.html.
Scheurig, A.C., Thorand, B., Fischer, B., Heier, M., & Koenig, W. (2008). Association between the intake of vitamins and trace elements from supplements and C-reactive protein: results of the MONICA/KORA Augsburg study. European Journal of Clinical Nutrition, 62(1), 127-37. https://doi.org/10.1038/sj.ejcn.1602687.
Sheikholeslami vatani, D., Ahmadi, S., Mojtahedi, H., Marandi, M., Ahmadi Dehrashid, K., Faraji, H., & Gharibi, F. (2011). Influence of different intensities of resistance exercise on inflammatory markers in young healthy men. Iranian Journal of Endocrinology and Metabolism; 12(6), 618-625. [In Persian]. URL: http://ijem.sbmu.ac.ir/article-1-885-en.html.
Shavandi, N., Saremi, A., & Tabibirad, S. (2013). The effect of aerobic training on vascular cell adhesion molecule and insulin resistance index in type 2 diabetes woman. Metabolism and Exercise, 3(1), 1-10. [In Persian].
Soltanian, Z., Vanaky, B., Ramezani fard, N., Shakeri, N., Shams, Z., & Fakhari Rad, F. (2019). Effect of eight weeks resistance training on gene expression of TNF-α and IL10 in the heart of type II diabetic male rats. Journal of Shahid Sadoughi University of Medical Sciences, 27(6), 1656-1667. [In Persian]. https://doi.org/10.18502/ssu.v27i6.1600
Taheri Kalani, A., & Nikseresht, M. (2015). The effect of 10 weeks resistance and aerobic training on inflammatory cytokines in sedentary overweight men. Journal of Ilam University of Medical Sciences, 23(5), 17-26. [In Persian]. URL: http://sjimu.medilam.ac.ir/article-1-2134-fa.html.
Teixeira, de.L., Jorge, O., João Páscoa, P.,& Flávio, R,. (2012). Regular physical exercise as a strategy to improve antioxidant and anti-inflammatory status: benefits in type 2 diabetes mellitus. Oxidative Medicine and Cellular Longevity, 2012, 2012:741545. https://doi.org/10.1155/2012/741545.
Tokuyama, A., Kanda, E., Itano, S., Kondo, M., Wada, Y., Kadoya, H., & Kashihara, N. (2021). Effect of zinc deficiency on chronic kidney disease progression and effect modification by hypoalbuminemia. Public Library of Science One, 16(5), e0251554. https://doi.org/10.1371/journal.pone.0251554
Zalewski, P.D. Ai., Q., Grosser, D., Jayaram. L., Murgia. C., & Ruffin. R. (2005). Zinc metabolism in airway epithelium and airway inflammation: basic mechanisms and clinical targets. A review. Pharmacology & Therapeutics, 105, 127-149. https://doi.org/10.1016/j.pharmthera.2004.09.004.
Zeraati, A., Sharifipour, F., Hoseinzadeh, M., Hasanzade, M., & Mamdoohi, F. (2015). Evaluation of correlation of high sensitive CRP with bioelectrical impedance parameters in patients undergoing hemodialysis. Medical Journal of Mashhad University of Medical Sciences, 57(8), 884-889. [In Persian]. https://doi.org/10.22038/mjms.2015.3582.
)