نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی دکترای فیزیولوژی ورزشی فرصت مطالعاتی پژوهشگاه علوم ورزشی، تهران، ایران.
2 استاد فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه اصفهان، اصفهان، ایران.
3 استاد گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران.
چکیده
زمینه و هدف: آتروفی عضلانی یک فرآیند فعال کنترل شده بوسیله مسیرهای پیام‌‌رسانی و برنامـه‌‌های رونویسی ویژه است. آتروژین-1/ MAFbx و MuRF1 در مدل‌‌های مختلف آتروفی عضلانی، به طور مثبت تنظیم می‌‌شوند و مسئول افزایش تجزیه پروتئین از طریق سیستم پروتئازوم یوبیکوئیتین هستند. هدف از این مطالعه بررسی تاثیر یک دوره تمرین مقاومتی با شدتهای بالا و متوسط بر بیان ژن‌‌های ژن‌‌های miRNA-23a، MuRF و آتروژین- 1 در عضلات تند و کند انقباض رت‌‌های نر سالمند نژاد ویستار بود. روش‌‌تحقیق: در این تحقیق 30 سر موش صحرایی سالمند (23 ماهه) نژاد ویستار به شکل تصادفی به سه گروه (10=n) شامل تمرین مقاومتی با شدت متوسط (MIRT)، تمرین مقاومتی با شدت بالا (HIRT) و کنترل تقسیم شدند. برنامه تمرین مقاومتی شامل هشت هفته تمرین بالا رفتن از نردبان با شدت زیاد و متوسط (به ترتیب با 80 و 60 درصد حداکثر ظرفیت حمل ارادی) و با تکرار پنج روز در هفته بود. جداسازی عضله نعلی و عضله خم کننده دراز شست پا بلافاصله بعد از خونگیری در شرایط استریل انجام شد. بیان ژن MuRF، آتروژین -1 و miR-23a با تکنیک RT-PCR اندازه‌‌گیری شد. تجزیه تحلیل آماری با استفاده از آزمون تحلیل واریانس یک طرفه و دو طرفه در سطح معنی‌‌داری 05/0>p صورت گرفت. یافته‌‌ها: بیان ژن‌‌های MuRF و آتروژین-1 پس از هشت هفته در دو گروه مقاومتی نسبت به گروه کنترل کاهش معنی‌‌دار و بیان miR-23a افزایش معنی‌‌داری پیدا کرد (05/0>p)؛ اما اختلاف تأثیر دو مداخله صورت گرفته بر miR-23a، MuRF و آتروژین-1 در هر دو عضله معنی‌‌دار نبود (05/0<p). نتیجه‌‌گیری: به نظر می‌‌رسد تمرین مقاومتی با شدتهای متوسط و بالا، می‌‌تواند باعث کاهش سطوح استراحتی عوامل مرتبط با آتروفی عضلانی و ژن‌‌های هدف آن در رت‌‌های سالمند شود و پیشرفت سارکوپنیا در دوره سالمندی را مهار نماید.
کلیدواژه‌ها
عنوان مقاله [English]
The effect of eight weeks of moderate and high intensity resistance training on miR-23a, Atrogin-1 and MuRF gene expression in fast and slow twitch muscles in Wistar older rats
نویسندگان [English]
- Zohreh Shanazari 1
- Mohammad Faramarzi 2
- Mohammadreza kordi 3
1 PhD Student in Exercise Physiology, Sport Sciences Research Institute Research Opportunity Student,, Tehran, Iran.
2 Full Professor, Department of Exercise Physiology, Faculty of Sport Sciences, University of Isfahan, Isfahan, Iran.
3 Full Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran.
چکیده [English]
Background and Aim: Muscle atrophy is an active process controlled by specific signaling pathways and transcriptional programs. Atrogin-1/MAFbx and MuRF1 are up-regulated in different models of muscle atrophy and is responsible for increased protein degradation through the ubiquitin proteasome pathway. The purpose of this study was to investigate the effect of moderate and high intensity resistance training on miR-23a, Atrogin-1 and MuRF gene expression in fast and slow twitch muscles in elderly rats. Materials and Methods: Thirty male Wistar rats (23 months old) were randomly divided into three groups (n=10) including moderate-intensity resistance training, high-intensity resistance training and the control groups. Resistance training were performed during eight weeks of climbing a ladder with high intensity (80% Maximum voluntary carrying capacity/MVCC) and moderate intensity (60% of MVCC) for 5 days a week. Isolation of Soleus muscle and Flexor halluces longus (FHL) was performed immediately after blood sampling in sterile conditions. MiR-23a, Atrogin-1 and MuRF expression were measured by RT-PCR method in Soleus and FHL muscles. The statistical analysis was performed by One-way & Two-way ANOVA tests with significance level of p<0.05. Results: The results showed that the expression of MuRF and Atrogin-1 genes in both resistance groups were significantly lower than those the control group and miR-23a expression expression in both high and moderate resistance groups was significantly higher than the control group (p<0.05). But there was no significant difference between the effect of high and moderate intensity resistance training on miR-23a, MuRF, and Atrogin-1 expression in both muscles (p>0.05). Conclusion: It seems that resistance training with moderate and high-intensity can decrease the resting levels and gene expression of factors associated with muscular atrophy (miR-23a) and its target genes (Atrogin-1, MuRF) in older rats, and these exercises could lead to the prevention of sarcopenia.
کلیدواژه‌ها [English]
- Resistance training
- miR-23a gene
- Atrogin-1 gene
- MuRF gene
- Aging
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