تأثیر یک دوره تمرین استقامتی توام با تزریق تستوسترون بر مقادیر کمرین و آپلین رت های دچار ایسکمی قلبی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دانشگاه سیستان و بلوچستان / گروه تربیت بدنی و علوم ورزشی

2 دانشگاه بیرجند / دانشکده تربیت بدنی و علوم ورزشی

3 دانشگاه شهید باهنر کرمان / دانشکده تربیت بدنی و علوم ورزشی

4 دانشگاه علوم پزشکی کرمان / مرکز تحقیقات قلب و عروق پژوهشکده علوم فیزیولوژی پایه و بالینی

5 دانشگاه کرمان / دانشکده تربیت بدنی و علوم ورزشی

چکیده

زمینه و هدف: توانبخشی و نوتوانی قلبی، از رویکردهای جدیدی است که به طرق مختلف با هدف افزایش عملکرد و کارآیی بیماران انجام می شود. هدف پژوهش حاضر بررسی تأثیر 12 هفته تمرین استقامتی توام با تزریق تستوسترون، بر مقادیر کمرین و آپلین در فرآیند توانبخشی رت های دچار ایسکمی قلبی بود. روش تحقیق:40 سرموش صحرایی نر نژاد ویستار (میانگین وزن 10±200 گرم و سن 8 هفته) در محیط کنترل شده نگهداری و به طور تصادفی در چهار گروه 10 تایی شامل گروه کنترل (C)، گروه تزریق تستوسترون پروپیونات (TP)، گروه تمرین استقامتی (ET) و گروه تمرین استقامتی و تزریق تستوسترون (ETTP)؛ تقسیم شدند. گروه تمرین استقامتی به مدت 12 هفته (5 روز در هفته) به تمرین پرداختند و گروه تزریق تستوسترون 3 روز در هفته به صورت درون صفاقی تستوسترون پروپیونات دریافت کردند. برای القا ایسکمی قلبی، ایزوپروترنول به صورت 2 روز متوالی و با فاصله 24 ساعت و دوز 50 میلی گرم/کیلوگرم وزن بدن به همه رت ها تزریق شد. بعد از اتمام 12 هفته تمرین و تزریق تستوسترون، رت ها با تزریق تیوپنتال سدیم بی هوش شدند و نمونه گیری خونی انجام شد. غلظت سرمی کمرین و آپلین با استفاده از روش الایزا اندازه گیری شد و داده های حاصل با استفاده از آزمون های تحلیل واریانس یک طرفه و آزمون تعقیبی LSD در سطح 05/0˂p تجزیه و تحلیل شدند. یافته ها: مقادیر کمرین در گروه های تمرین استقامتی (008/0=p)، تزریق تستوسترون (03/0=p) و تمرین استقامتی توام با تزریق تستوسترون (01/0=p) به طور معنی داری کاهش یافت؛ در مقابل، افزایش معنی داری در مقادیر آپلین در گروه های تمرین استقامتی (001/0=p)، تزریق تستوسترون (001/0=p) و تمرین استقامتی توام با تزریق تستوسترون (001/0=p) مشاهده شد. نتیجه گیری: استفاده از تزریق تستوسترون و تمرین استقامتی در فرآیند توان بخشی، بهبود عوامل کلیدی درگیر در سلامت قلب و عروق را به دنبال دارد.

کلیدواژه‌ها


عنوان مقاله [English]

The effects of endurance training with testosterone injection on Chemerin and Apelin levels in rats with ischemic heart disease

نویسندگان [English]

  • Sadollah Salarmohammadi 1
  • Mehdi Mogharnasi 2
  • Hamid Marefati 3
  • Soheil Aminizadeh 4
  • Mahnaz Hajghani 5
چکیده [English]

Background and Aim: Cardiac rehabilitation is a new approach in various ways to increase performance and efficiency of patient. This study examined the effect of 12 weeks of endurance training with testosterone injections on Chemerin and Apelin levels in the process of rehabilitation in male adult rats with ischemic heart disease. Materials and Methods: 40 Wistar male rats (average weight 200 ± 10 g and 8 weeks old) were randomly divided into four groups (n=10) and stored in a controlled environment : 1) control group (C), 2) injection of testosterone propionate group ( TP), 3) endurance training group (ET) and 4) Endurance Training with injections of testosterone Propionate (ETTP) . Endurance training group exercised for 12 weeks (5 days/week) and testosterone propionate group were given Testosterone propionate injections (3 times/week). Isoproterenol (50 mg/kg) was dissolved in normal saline and was injected intraperitoneally (i.p.) during two days within 24 hours in order to create empirical myocardial infarction. After 12 weeks, rats anesthetized with sodium thiopental (50 mg/kg) and blood samples were taken and chemerin and apelin levels were measured by using ELISA method and data analysis was done using one-way ANOVA and post-hoc LSD tests. Results: The results revealed a significant reduction in the amount Chemerin in endurance training (p=0.008), injected testosterone (p=0.03) and endurance training with testosterone injections (p=0.01) knows shown and the significant increase significant amounts of apelin in endurance training (p=0.001), injected testosterone (p=0.001) and endurance training with testosterone injections (p=0.001) was observed. Conclusion: This study shows that injection of testosterone and endurance training in the process of rehabilitation is feasible and can have a positive effect on cardiovascular health.

کلیدواژه‌ها [English]

  • Endurance training
  • Testosterone
  • Apelin
  • chemerin
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