اثر 8 هفته تمرین استقامتی و مهار کننده نیتریک اکساید بر آپلین بافت چربی در رت های نر سالمند

نوع مقاله: مقاله پژوهشی

نویسندگان

1 کارشناس ارشد فیزیولوژی ورزشی واحد آیت الله آملی/ دانشگاه آزاد اسلامی/ آمل

2 استادیار گروه فیزیولوژی ورزشی واحد آیت الله آملی/ دانشگاه آزاد اسلامی/ آمل

چکیده

زمینه و هدف: آپلین توسط بافت چربی ترشح  می شود و نقش مهمی در فرآیندهای التهابی ایفا می کند و به نظر می رسد به عنوان یک سایتوکاین ضد التهابی عمل می کند. هدف از اجرای این پژوهش بررسی اثر هشت هفته تمرین استقامتی و مهار کننده نیتریک اکساید بر آپلین بافت چربی، گلوکز و انسولین در موش­ های صحرایی نر سالمند بود. روش تحقیق: به همین منظور 24 سر رت نر با نژاد ویستار 22 ماهه از انستیتو رازی خریداری و به مرکز تحقیقات منتقل شدند که به صورت تصادفی به چهار گروه کنترل، تمرین، مهارکننده و مهارکننده-تمرین تقسیم شدند. دوره تمرینی به مدت 8 هفته اجرا شد که هر هفته 5 روز و شدت تمرین 80-75 درصد حداکثر اکسیژن مصرفی بود. گروه مهارکننده- تمرین همراه با دوره 8 هفته ای تمرینات، به صورت محلول با آب آشامیدنی به مقدار 0/75 اختلاف معنی داری میلی گرم/ میلی لیتر (معادل 100 میلی گرم به ازای هر کیلوگرم وزن موش ها در روز) L-NAME دریافت کردند. گروه مهارکننده طی دوره تنها مکمل دریافت نمودند. تمامی رت ها 72 ساعت پس از آخرین جلسه تمرینی و بعد از 24 ساعت ناشتایی کشته و نمونه های بافت چربی جمع آوری گردید. تجزیه و تحلیل داده ها با استفاده از روش آماری آنالیز واریانس یک طرفه و آزمون توکی در سطح معنی داری 0/05>p انجام شد. یافته‌ها: نتایج نشان داد هشت هفته تمرین استقامتی موجب افزایش معنی­ دار سطح آپلین در بافت چربی رت های نر سالمند شد (0/000=p). با این وجود، تغییری در سطوح گلوکز (0/157=p) و انسولین (0/832=p) پس از دوره تمرینات استقامتی در گروه ها مشاهده نشد. نتیجه ­گیری: به نظر می رسد تمرین استقامتی می تواند سطح آپلین بافت چربی را  مستقل از انسولین و گلوکز افزایش دهد. بنابراین احتمالاً تمرین استقامتی بتواند در کنترل وضعیت التهابی آزمودنی­ های سالمند نقش داشته باشد.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of 8 weeks of endurance training and L-NAME on Apelin in adipose tissue in elderly male’s rats

نویسندگان [English]

  • Fatemeh Fani 1
  • Asieh Abbassi Daloii 2
  • Ahmad Abdi 2
چکیده [English]

Background and Aim: Apelin that is secreted by adipose tissue plays an important role in the inflammation process and it seems could be considered as an anti-inflammatory cytokines. The aim of this study was to determine the effect of 8 weeks of endurance training and L-NAME on Apelin in adipose tissue, glucose and insulin in elderly male’s rats. Materials and Methods: For this purpose, 24 vistar elderly rats with average 22 months old purchased from Razi Institute and transferred to Research Center, they  randomly divided into four groups as: control, training, L-NAME, training and L-NAME. Training protocol performed for 8 weeks and 5 days a week with 75-80 VO2max. Inhibitor-training Group with 8-week training period, were given the solution to the drinking water 75/0 mg / ml (100 mg kg mice per day) L-NAME. Inhibitors Group during the period received only supplement. All rats were killed 72 hours after the final training session and after 24 hours of fasting adipose tissue samples were collected and kept in -80 Celsius degree. Also, Data was analyzed with One way ANOVA and Tucky in p<0.05. Results: The results showed that the eight weeks of endurance exercise significantly increased the level of apelin in adipose tissue of older male rats (p=0.000). However, no changes was observed in the levels of glucose (p=0.157) and insulin (p=0.832) after endurance training in groups.
 Conclusion: It seems that endurance training can increase the levels of apelin in adipose tissue independently of insulin and glucose. So probably endurance training could have a role in control inflammatory condition in elderly subjects.

کلیدواژه‌ها [English]

  • Endurance training
  • L-NAME
  • Apelin in adipose tissue
  • Elderly male rats

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