تاثیر فعالیت مقاومتی حاد بر سطوح سرمی برخی شاخص های التهابی و آسیب عضلانی در زنان غیر فعال

نوع مقاله: مقاله پژوهشی

نویسندگان

1 کارشناس ارشد فیزیولوژی ورزشی، دانشگاه آزاد اسلامی، واحد گناباد، ایران

2 استادیار گروه علوم ورزشی، دانشگاه بجنورد، بجنورد، ایران

3 استادیار گروه بیوشیمی بالینی، دانشگاه علوم پزشکی گناباد، گناباد، ایران

چکیده

زمینه و هدف: مطالعات نتایج متناقضی را پیرامون اثر حاد تمرین ورزشی بر شاخص­های التهابی، آسیب اکسایشی و آسیب عضلانی مطرح ساخته­اند. از این رو، هدف مطالعه حاضر بررسی اثر حاد فعالیت مقاومتی شدید بر سطوح سرمی عامل نکروز دهنده تومور آلفا (TNF-α) به عنوان شاخص التهابی، مالون دی آلدهید (MDA) به عنوان شاخص فشار اکسایشی، و لاکتات دهیدروژناز (LDH) و کراتین کیناز (CK) به عنوان دو شاخص آسیب عضلانی بود. روش تحقیق: بدین منظور 20 زن سالم غیرفعال (میانگین سن 1/56±21/55 سال و شاخص توده بدنی 3/26±21/68 کیلوگرم بر متر مربع) به طور تصادفی به دو گروه فعالیت مقاومتی شدید و کنترل تقسیم شدند. آزمودنی­ها فعالیت مقاومتی حاد را با شدت 85 درصد یک تکرار بیشینه در 8 ایستگاه (پرس سینه، پرس پا، قایقی نشسته، پرس بالای سر، باز کردن  زانو، باز کردن بازو، خم کردن بازو و بلند کردن پاشنه) در 3 نوبت انجام دادند. قبل و بلافاصله بعد از فعالیت مقاومتی، نمونه­های خونی جمع آوری شدند. با استفاده از کیت­های تجاری متغیرهای MDA و TNF-α به روش الایزا اندازه­گیری شدند و سطوح LDH و CK به روش فتومتریک مورد سنجش قرار گرفتند. از آزمون tوابسته و مستقل به ترتیب برای مقایسه درون گروهی و بین گروهی متغیرهای وابسته در سطح (0/05>p) استفاده شد. یافته­ها: یک جلسه فعالیت مقاومتی شدید موجب افزایش معنی­دار (0/001=p) سطوح سرمی TNF-α  و MDA شد. همچنین، سطوح سرمی شاخص­های آسیب عضلانی LDH (p=0/03) و CK(p=0/001) متعاقب فعالیت مقاومتی حاد به طور معنی­داری افزایش یافت. نتیجه گیری: یک جلسه فعالیت مقاومتی شدید با آسیب اکسایشی و عضلانی در زنان غیرفعال همراه است و اتخاذ تدابیر تمرینی و تغذیه­ای هنگام اجرای این نوع تمرینات، ضروری به نظر می­رسد.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of acute resistance exercise on serum levels of some inflammatory and muscle damage markers in inactive women

نویسندگان [English]

  • Mahboobe Ajam Zibad 1
  • Hossein TaheriChadorneshin 2
  • Seyed Hosein Abtahi Eivary 3
1 MSc in Exercise Physiology, Islamic Azad University, Gonabad Branch, Iran
2 Assistant Professor of Sport Sciences Department, University of Bojnord, Bojnord, Iran
3 Assistant Professor, Clinical Biochemistry Department, Gonabad University of Medical Sciences, Gonabad, Iran
چکیده [English]

Background and Aim: Studies have reported conflicting results regarding the acute effects of exercise on inflammatory, oxidative stress and muscle damage parameters. Therefore, this study aimed to investigate the effect of acute resistance exercise on serum levels of tumor necrosis factor alpha (TNF-α), as a marker of inflammation, malondialdehyde (MDA), as a marker of oxidative stress, and levels of lactate dehydrogenase (LDH) and creatine kinase (CK), as two markers of muscle damage. Materials and Methods: For this purpose, 20 healthy and inactive women (age, 21.55+1.56 year; BMI, 21.68+3.26 kg/m2) were randomly divided into acute resistance exercise and control groups. The subjects performed one bout of acute resistance exercise with 85% of one-repetition maximum (1RM) at 8 stations (bench press, leg press, seated rows, overhead press, seated leg press, arm extension, arm curls and calf raises), each exercise were done for 3 sets. Blood samples were taken before and immediately after exercise. TNF-α and MDA levels were measured using ELISA method by commercial kits. Moreover, LDH and CK were measured by photometric method. Paired sample t-test and independent t-tests were used to analyzed dependent variable within and between groups, respectively (p<0.05). Results: One bout of acute resistance exercise had significant increases (p=0.001) in serum levels of TNF-α and MDA. Also, serum levels of muscle damage parameters, LDH (p=0.03) and CK (p=0.001), showed significantly increases following acute resistance exercise. Conclusion: One bout of intense resistance exercise is associated with oxidative and muscle damage in inactive women, therefore adoption of training and nutritional approaches are essential when implementing with this kind of exercise.

کلیدواژه‌ها [English]

  • Acute resistance exercise
  • Tumor necrosis factor alpha
  • Malondialdehyde
  • Lactate Dehydrogenase
  • Creatine Kinase
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