اثر 8 هفته تمرین استقامتی تناوبی ترکیبی بر مقادیر پلاسماییIL-10 ،TNF-a ، مقاومت انسولین و نیمرخ چربی در نوجوانان پسر

نوع مقاله: مقاله پژوهشی

نویسندگان

1 استادیار گروه علوم ورزشی، دانشکده علوم تربیتی و روانشناسی دانشگاه شهید مدنی آذربایجان، تبریز، ایران

2 استادیار گروه تربیت بدنی و علوم ورزشی دانشکده علوم انسانی، دانشگاه زنجان، ایران

چکیده

زمینه و هدف: سایتوکاین های پیش التهابیعامل نکروز توموری آلفا (TNF-α) و ضد التهابی  اینترلوکین-10 (IL-10) به ترتیب نقش مهمی در ایجاد و جلوگیری از التهاب سیستمیک و بروز بیماری های ناشی از چاقی از جمله بیماری عروق کرونر (CAD) دارند. هدف این مطالعه بررسی تاثیر هشت هفته تمرین استقامتی تناوبی ترکیبی بر مقادیر پلاسمایی TNF-α، IL-10، مقاومت انسولینی و نیمرخ چربی در نوجوانان پسر دارای اضافه وزن و چاق بود. روش تحقیق: در این مطالعه نیمه تجربی 28 آزمودنی (سن 1/89±16/93 سال، وزن 9/98±88/07 کیلوگرم و شاخص توده بدنی 2/55±28/35 کیلوگرم بر مترمربع) به طور تصادفی ساده انتخاب و در دو گروه تجربی (13 نفر) و کنترل (15 نفر) قرار گرفتند. پروتکل تمرین به مدت 8 هفته، 4 روز در هفته، 70 دقیقه در هر جلسه اجرا شد. نمونه های خونی قبل و بعد تمرین جهت ارزیابی متغیرهای مورد نظر گرفته شد. سطوح پلاسمائی TNF-α وIL-10  با استفاده روش الایزای ساندویچی با کیت های تجاری اندازه گیری شدند. داده­ها با آزمون آماری t مستقل و ضریب همبستگی پیرسون در سطح معنی داری pیافته ها: نتایج نشان داد بعد از مداخله تمرین مقادیر پلاسمائی TNF-α و IL-10 گروه تجربی در مقایسه با گروه کنترل تغییر معنی دار نداشت (به ترتیب 0/32= p و 0/53= p ) اما مقادیر متغیرهای شاخص مقاومت انسولینی، تری گلسیرید، کلسترول تام، نسبت لیپوپروتئین کم چگال به لیپوپروتئین پرچگال، نسبت کلسترول تام به لیپوپروتئین پرچگال، درصد چربی بدن، شاخص توده بدنی و اندازه دور کمر در گروه تجربی در مقایسه با گروه کنترل در اثر تمرین، کاهش  معنی  دار و حداکثر اکسیژن مصرفی، افزایش معنی داری داشت p<0.05)). نتیجهگیری: به نظر می رسد تمرین استقامتی تناوبی ترکیبی به مدت 8 هفته برای کنترل وزن، کاهش چربی های خون و بهبود آمادگی هوازی مناسب است، اما پتانسیل ایجاد تغییرات مطلوب در سایتوکاین هایی مثل TNF-α و IL-10 را ندارد. احتمالاً انجام این تمرینات با شدت و حجم بالا کارساز خواهد بود؛ موضوعی که نیاز به مطالعات بیشتر دارد.

کلیدواژه‌ها


عنوان مقاله [English]

The effects of 8 weeks interval endurance combined training on plasma TNF-α, IL-10, insulin resistance and lipid profile in boy adolescents

نویسندگان [English]

  • Bahloul Ghorbanian 1
  • Aghaali Ghasemnian 2
1 Physical Education and Sports Sciences Department, Faculty of Humanities, University of Zanjan, Zanjan, Iran
2 Assistant Professor of Exercise Physiology, Physical Education and Sports Sciences Department, Faculty of Humanities, University of Zanjan, Zanjan, Iran
چکیده [English]

Background & Aim: Tomor necrosis factor alpha (TNF-α; as a pro-inflammatory cytokine) and interlokin-10 (IL-10; as
an anti-inflammatory cytokine) have an important role in the development and the prevention of systemic inflammation and incidence of obesity-induced diseases such as CAD, respectively. The aim of this study was to investigate the effects
of 8-week interval endurance combined training (IECT) on plasma TNF-α, IL-10, insulin resistance, and lipid profiles in
overweight and obese boy adolescents. Materials and Methods: In this semi-experimental study, 28 healthy overweight
and obese boy adolescents (16.93 ± 1.89 yr., 88.07 ± 9.98 kg. and 28.35 ± 2.55 kg/m²) were volunteered and randomly
assigned into training (n= 13) and control (n= 15) groups. Exercise protocol was IECT (8 WK, 4 d/wk, 70 min/d). Blood
samples were taken before and after exercise to assess the variables. Serum levels of TNF-α and IL-10 were measured
by sandwich ELISA method. T-test and Pearson correlation coefficient was employed. The criterion of statistical significance was set as p0.05). Moreover, the amounts of insulin resistance, triglyceride, total cholestrol; low density lipoprotein, low density lipoprotein/high density lipoprotein, total cholesterol/high density lipoprotein, body fat percent, body mass index, and waist circumference were significantly decreased and VO2max significantly increased (p<0.05).
Conclusion: It seems that a combination of interval endurance training for 8 weeks can be considered to weight controling, reducing blood lipids and improving aerobic fitness, but to create favorable changes in cytokines, such as TNF-α and IL-10 is not sufficient. Probably, it is more useful with more intensity and volume. To achieve more accurate results, more studies are needed in this area.

کلیدواژه‌ها [English]

  • IL-10
  • TNF-α
  • Interval combined exercises training
  • Overweight and obese adolescent

Ahmadizad, S., Haghighi, A.H., & Hamedinia, M.R. (2007). Effects of resistance versus endurance training on
serumadiponectin and insulin resistance index. European Journal of Endocrinology, 15(7), 625-31.
Ashton Acton, Q. (2011). Issues in Physiology, Cell Biology, and Molecular Medicine. Scholarly Editions.
Butcher, LR., Thomas, A., Backx K., Roberts, A., Webb, R., & Morris, K. (2008). Low-Intensity Exercise Exerts Beneficial
Effects on Plasma Lipids via PPAR [gamma]. Medicine and Science in Sports and Exercise, 40(7),1263 -70

de Waal Malefyt, R., Haanen, J., Spits, H., Roncarolo, MG., Te Velde, A., Figdor, C., Johnson, K., Kastelein,
R., Yssel, H., & de Vries, JE. (1991). Interleukin 10 (IL-10) and viral IL-10 strongly reduce antigenic-specific human
T cell proliferation by disminishing the antigenic-presenting capacity of monocytes via downregulation of class II
major histocompatibility complex expression. Journal of Experimental Medicine, 174, 915-24.
Galedari, M., & Azarbayejani, M.A., (2015). The effect of training mode during calorie restriction on plasma adipokines and fasting insulin in obese men. Sport Physiology, 24, 121-138. [Persian]
Gene, Adams. (2002). Exercise Physiology Laboratory Manual. 4th Edition. McGraw-Hill Publishers, New York.
Ghanbari-Niaki, A., Saghebjoo, M., & Hedayati, M. (2011). A single session of circuit-resistance exercise effects on
human peripheral blood lymphocyte ABCA1 expression and plasma HDL-C level. Regulatory Peptides, 166 (1-3), 42-7.
Goldhammer, E., Tanchilevitch, A., Maor, I., Beniamini, Y., Rosenschein, U., & Sagiv, M. (2004). Exercise training
modulates cytokines activity in coronary heart disease patients. Journal of the American Geriatrics Society, 52(7),
1098-104
Haghighi, A.H. (2005). The effects of endurance and resistance training on inflammatory cytokines and insulin resistance
in obesity men. Ph.D Thesis, Physical Education Department, Faculty of Physical Education & Sport Sciences. Tehran University. [Persian]
Haskell, W.L., Lee, I.M., Pate, R.R., Powell, K.E., Blair, S.N., Franklin, B.A., Macera, C.A., Heath, G.W., Thompson,
P.D., & Bauman, A. (2007). Physical Activity and Public Health. Updated Recommendation for Adults from the
American College of Sports Medicine and the American Heart Association. Medicine & Science in Sports & Exercise,
39(8), 1423-34.
Jackson, A.S., & Pollock, M.L. (1978). Generalized equations for predicting body density of men. British Journal of
Nutrition, 40, 497–504.
Jung, S.H., Park, H.S., Kim, K.S., Choi, W.H., Ahn, C.W., & Kim, B.T. (2008). Effect of weight loss on some serum
cytokines in human obesity: increase in IL-10 after weight loss. Journal of Nutritional Biochemistry, 19(6), 371-5.
Kadoglou, N.P., Iliadis, F., Angelopoulou, N., Perrea, D., Ampatzidis, G., Liapis, C.D., & Alevizos, M. (2007). The anti-inflammatory effects of exercise training in patients with type 2 diabetes mellitus. European Journal of Preventive Cardiology, 14(6), 837-43.
Kern, P.A., Saghizadeh, M., Ong, J.M., Bosch, R.J., Deem, R., & Simsolo, R.B, (1996). IRS-1-mediated inhibition of
insulin receptor tyrosine kinase activity in TNF-alpha and obesity-induced insulin resistance. Science, 271(5249),
665-8.
Kim, ES., Im, J.A., Kim, K.C., Park, J.H., Suh, S.H., Kang, E.S., Kim, S.H., Jekal, Y., Lee, C.W., Yoon, Y.J., Lee, H.C., &
Jeon, J.Y. (2007). Improved insulin sensitivity and adiponectin level after exercise training in obese Korean youth.
Obesity, 15(12), 3023-30.
Kraus, W.E., Houmard, J.A., Duscha, B.D., Knetzger, K.J., Wharton, M.B., McCartney, J.S., Bales, C.W., Henes,
S., Samsa, G.P., Otvos, J.D., Kulkarni, K.R., & Slentz C.A. (2002). Effects of the amount and intensity of exercise on
plasma lipoproteins. New England Journal of Medicine, 347(19),1483-92.
Lucas, S.R., & Platts-Mills, T.A. (2005). Physical activity and exercise in asthma: Relevance to etiology and treatment. The
Journal of Allergy and Clinical Immunology, 11, 928-934. 

Malin, S.K., Gerber, R., Chipkin, S.R., & Braun, B. (2012). Independent and combined effects of exercise training and
metformin on insulin sensitivity in individuals with prediabetes. Diabetes Care, 35(1), 131-6.
Maqsodi, N., Khosravi, N., & Ravasi, A.A. (2010). The effects of aerobic and strength training on some serum
cytokines in men and women MS patients. Journal of Sport Biosciences, 10, 5-13. [Persian]
Michael, G., Bishop, N.C., Stensel, D.J., Lindley, M.R., Mastana, S.S. & Nimmo, M.A. (2011). The anti-inflammatory effects of exercise: mechanisms and implications for the prevention and treatment of disease. Nature Reviews Immunology, 11, 607-15.
Mirmohammadi, S., Hafezi, R., Mehrparvar, A., Rezaeian, B., & Akbari, H. (2001). Prevalence of overweight and obesity
among Iranian school children in different ethnicities. Iranian Journal of Pediatrics, 21(4), 515-520.
Norma, E. D. (2005). Atherosclerosis: An Inflammatory Process. Journal of Insurance Medicine, 37, 72–75.
O’Leary, V.B., Marchetti, C.M., Krishnan, R.K., & Kirwan, J.P. (2006). Exercise-induced reversal of insulin resistance in
obese elderly is associated with reduced visceral fat. Journal of Applied Physiology, 100, 1584–89.
Qorzi, A., Aghaalinejad, H., Rajabi, H., Azad, A., Molanori, S.H., & Hadayati, M. (2011). The effects of ten weeks
resistance and endurance training on hormonal, lipids and inflammatory indices in non-athletes men. Iranian
Journal of Endocrinology & Metabolism, 13(6), 614-620. [Persian]
Shearman, J., Dominic, M., Jane, H., & Michael, H. (2010). The Effect of Physical Activity on Serum Lipids, Lipoprotein,
and. Apolipoproteins. Archives of Exercise in Health and Disease, 1(2), 43-49.
Stensvold, D., Slordahl, S.A., & Wisloff, U. (2012). Effect of exercise training on inflammation status among people with
metabolic syndrome. Metabolic Syndrome and Related Disorders, 10(4), 267-72.
Stewart, L.K., Flynn, M.G., Campbell, W.W., Craig, B.A., Robinson, J.P., Timmerman, K.L., McFarlin, B.K., Coen, P.M., &
Talbert, E. (2007). The influence of exercise training on inflammatory cytokines and C-reactive protein. Medicine
& Science in Sports & Exercise, 39(10), 1714-9.
Tongjian, Y., Barbara, J., & Nicklas, A. (2008). Effects of exercise on adipokines andthe metabolic syndrome. Current
Diabetes Reports, 8, 7-11.
Touvra, A.M., Volakils, K.A., Spassis, A.T., Zois, CE., Douda, H.D., Kotsa, K., & Tokmakidis, S.P. (2011). Combiened
strength and aerobic training increase transforming growth factor-β1 in patients with type 2 diabetes. Hormones, 10
(2), 125-30.
Tsukui, S., Kanda, T., Nara, M., Nishino, M., Kondo, T., & Kobayashi, I. (2000). Moderate-intensity regular exercise decreases serum tumor necrosis factor-alpha and HbA1c levels in healthy women. International Journal of Obesity and Related Metabolic Disorders, 24(9), 1207-11.
Uyemura, K., Demer, L.L., Castle, S.C., Jullien, D., Berliner, J.A., Gately, M.K., Warrier, R.R., Pham, N., Fogelman,
A.M., & Modlin R.L. (1996). Cross regulation roles of interleukin (IL)-12 and IL-10 in the atherosclerosis. Journal of
Clinical Investigation, 97, 2130-8.
Yin, K., Liao, D.F., & Tang, C.K. (2010). ATP-binding membrane cassette transporter A1 (ABCA1): a possible link
between inflammation and reverse cholesterol transport. Molecular Medicine, 16(9-10), 438-49.
Zhang, X., Shu, X.O., Gao, Y.T., Yang, G., Matthews, C.E., Li, Q,. Li, H., Jin, F., & Zheng W. (2004). Anthropometric predictors of coronary heart disease in Chinese women. International Journal of Obesity and Related Metabolic Disorders, 28(6), 734-40.