تاثیر تمرین استقامتی شنا بر تغییرات شاخص آپوپتوزی و غلظت متالوتیونین کبدی موش های باردار در معرض مسمومیت کادمیوم

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشیار گروه فیزیولوژی ورزشی/ دانشکده تربیت بدنی و علوم ورزشی/ دانشگاه مازندران/ بابلسر

2 کارشناس ارشد فیزیولوژی ورزشی/ دانشکده تربیت بدنی و علوم ورزشی/ دانشگاه مازندران/ بابلسر

3 استادیار دانشکده دامپزشکی دانشگاه تبریز/ تبریز

4 استادیار بیوشیمی/ پژوهشکده غدد و متابولیسم/ دانشگاه علوم پزشکی شهید بهشتی/ تهران

چکیده

زمینه و هدف: متالوتیونین نقش مهمی در کنترل آپوپتوزیس، دفع فلزات سنگین از بدن و انتقال عناصر ضروری از مادر به جنین ایفا م یکند. هدف پژوهش حاضر بررسی تاثیر تمرین شنای استقامتی بر غلظت متالوتیونین و شاخص آپوپتوزی کبد مو شهای صحرایی باردار در معرض مسمومیت با کادمیوم بود. روش تحقیق:32 موش صحرایی باردار(20±200 گرم) به چهار گروه (کنترل، تمرین، کادمیوم، تمرین + کادمیوم) تقسیم شدند. کادمیوم کلراید به صورت محلول در آب خوراکی به میزان 400 میلی گرم بر هر کیلوگرم وزن بدن از روز اول بارداری تا روز زایمان داده شد. پروتکل تمرین شامل شنا به مدت 60 دقیقه در روز، 5 روز در هفته در طی بارداری بود. نمونه گیری بافتی از کبد مو ش های صحرایی دو روز پس از زایمان انجام شد. غلظت متالوتیونین و شاخص آپوپتوزی کبدی به ترتیب با استفاده از روش الایزا و تکنیک ایمونوهیستوشیمی تانل به روش غیر رادیواکتیو نشاندار کردن انتهایی در جای خود تعیین شد. برای تجزیه و تحلیل یافته های پژوهش از آزمون آنالیز واریانس یک طرفه و آزمون تعقیبیLSD در سطح معناداری ( p ≤0/05)استفاده گردید. یافته ها: تمرین استقامتی شنا آپوپتوزیس کبد ناشی از کادمیوم را به طور معن یداری کاهش داد (005/p =0)، اما بر تغییرات غلظت متالوتیونین کبدی تاثیر معنی داری نداشت. نتیجه گیری: اگر چه تمرین استقامتی شنا تاثیر معنی داری بر افزایش متالوتیونین کبد ندارد، احتمالا از مسیرهای دیگری شاخص آپوپتوزی را کاهش می دهد و با مرگ سلولی ناشی از کادمیوم مقابله می کند.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of swimming endurance training on changes in liver apoptotic index and metallothionein levels in pregnant rats exposed to cadmium

نویسندگان [English]

  • Shadmehr Mirdar 1
  • Narges Musavi 2
  • Gholamreza Hamidian 3
  • Mehdi Hedayati 4
چکیده [English]

Background and Aim: Metallothionein plays important role in control of apoptosis, heavy metals elimination from body, and trace element transportation from mother to fetus. The aim of current study was to investigate the effects of swimming endurance training on induction of liver hepatic metallothionein (MT) in pregnant rats exposed to cadmium poisoning. Materials and Methods: Thirty-two pregnant rats (200 ± 20 g) were divided into four groups (control, swimming training, cadmium, and swimming training+ cadmium). Cadmium chloride was given orally (400 mg/kg in drinking water) from the first day of pregnancy until delivery. Training protocol was included 60 minutes swimming for 5 days a week during pregnancy. Liver tissues were removed two days after delivery. Liver MT levels and apoptotic index were determined by ELISA method and nonradioactive in situ end labeling method using TUNEL immunocytochemical technique, respectively. The ANOVA and post hoc LSD tests were used to analyze the data of study at p ≤ 0.05. Results: Swimming endurance training significantly decreased cadmium-induced apoptosis (p =0.005), but had no effect on liver MT levels. Conclusion: Although, swimming endurance training had no effect on liver MT levels, but decreased cadmium-induced apoptosis presumably via other mechanism than induction of liver MT. Thus, it was somewhat effective to contrast with cadmiuminduced cell death.

کلیدواژه‌ها [English]

  • Metallothionein
  • Cadmium
  • Pregnant Rat
  • Swimming Endurance Training
  • Apoptotic Index

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