عنوان مقاله [English]
نویسندگان [English]چکیده [English]
Background and Aim: Metallothionein plays important role in control of apoptosis, heavy metals elimination from body, and trace element transportation from mother to fetus. The aim of current study was to investigate the effects of swimming endurance training on induction of liver hepatic metallothionein (MT) in pregnant rats exposed to cadmium poisoning. Materials and Methods: Thirty-two pregnant rats (200 ± 20 g) were divided into four groups (control, swimming training, cadmium, and swimming training+ cadmium). Cadmium chloride was given orally (400 mg/kg in drinking water) from the first day of pregnancy until delivery. Training protocol was included 60 minutes swimming for 5 days a week during pregnancy. Liver tissues were removed two days after delivery. Liver MT levels and apoptotic index were determined by ELISA method and nonradioactive in situ end labeling method using TUNEL immunocytochemical technique, respectively. The ANOVA and post hoc LSD tests were used to analyze the data of study at p ≤ 0.05. Results: Swimming endurance training significantly decreased cadmium-induced apoptosis (p =0.005), but had no effect on liver MT levels. Conclusion: Although, swimming endurance training had no effect on liver MT levels, but decreased cadmium-induced apoptosis presumably via other mechanism than induction of liver MT. Thus, it was somewhat effective to contrast with cadmiuminduced cell death.
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