تاثیر تمرین استقامتی شنا و مکمل سیلیمارین در طی بارداری بر شاخص آپوپتوزی سلول های کبد نوزادان موش های صحرایی ناشی از مسمومیت مادری با کادمیوم

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشیار فیزیولوژی ورزش/ دانشکده تربیت بدنی و علوم ورزشی/ دانشگاه مازندران/ بابلسر

2 کارشناس ارشد تربیت بدنی/ دبیرستان شهید مطهری/ اداره آموزش و پرورش شهرستان بابل

3 استادیار بافت شناسی گروه علوم پایه/ دانشکده دامپزشکی/ دانشگاه تبریز/ تبریز

4 کارشناس ارشد تربیت بدنی/ دانشکده تربیت بدنی و علوم ورزشی/ دانشگاه مازندران/ بابلسر

چکیده

زمینه و هدف: در طی رشد جنین، هم تکثیر سلولی و هم آپوپتوز برای رشد صحیح بافت کبد جنین ضروری است. این مطالعه با هدف بررسی تاثیر یک دوره برنامه تمرین استقامتی شنا و مکمل سیلیمارین در طی بارداری بر شاخص آپوپتوزی سلول های کبد نوزادان مو شهای صحرایی در معرض مسمومیت با کادمیوم انجام گرفت. روش تحقیق:  72 سر موش صحرایی باردار نژاد ویستار (وزن 20 ± 200 گرم) به 9 گروه تقسیم شدند. کادمیوم کلراید به میزان 400 میلی گرم بر کیلوگرم وزن بدن به صورت محلول در آب آشامیدنی به مو شها خورانده شد. برنامه تمرینی در دوران بارداری 60 دقیقه شنا در روز و به مدت 5 روز در هفته بود. سیلیمارین (100 میلی گرم بر کیلوگرم وزن بدن) به صورت زیرجلدی 3 بار در هفته تزریق شد. نمونه برداری از بافت کبد دو روز پس از تولد انجام شد و برای تعیین شاخص آپوپتوزی کبد از تکنیک ایمونوهیستوشیمی تانل به روش غیر رادیواکتیو نشاندار کردن انتهایی در جای خود، استفاده گردید. برای تجزیه و تحلیل یافت هها از تحلیل واریانس یک طرفه و آزمون تعقیبی LSD در سطح خطای 0/05≥α  بهره برداری گردید. یافته ها: شاخص آپوپتوزی کبد در گروه های کادمیوم سیلیمارین و کادمیوم  تمرین  سیلیمارین در مقایسه با گروه کادمیوم، کاهش معنی داری (0/001>p) نشان داد؛ اما تمرین شنا به تنهایی تاثیری بر شاخص آپوپتوزی کبد نداشت (0/83=p ). نتیجه گیری: تمرین استقامتی شنا در طی بارداری تاثیری بر کاهش شاخص آپوپتوزی سلول های کبد نوزادان ناشی از مسمومیت مادری با کادمیوم ندارد، اما تزریق سیلیمارین به طور احتمالی آن را کاهش می دهد.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of swimming endurance training and silymarin supplementation during pregnancy on maternal cadmium exposure – induced apoptosis in hepatocytes of rat neonates

نویسندگان [English]

  • Shadmehr Mirdar Harijani 1
  • Hossein Ali asgharzade Oliaei 2
  • Gholamreza Hamidian 3
  • Narges Musavi 4
چکیده [English]

Background and Aim: Both cell proliferation and apoptosis are required for proper development of the neonatal parenchyma of the liver. This study was conducted to evaluate the effects of swimming endurance training and silymarin supplementation during pregnancy on maternal cadmium – induced apoptosis in hepatocytes of neonates. Materials and Methods: 72 pregnant Wistar rats (200 ± 20 g) were divided into 9 groups. Cadmium chloride was given orally (400 mg/kg in drinking water) from the first day of pregnancy. Training protocol was included 60 minutes swimming for 5 days per week during pregnancy. Silymarin (100 mg/kg) was injected subcutaneously three times a week. Liver tissue was removed two days after born and apoptotic index also determined by nonradioactive in situ end labeling method using TUNEL immunocytochemical technique. The ANOVA and post hoc LSD tests were used to analyze the collected data at p≤ 0.05. Results: Apoptotic index of hepatocytes were decreased significantly in cadmium- silymarin and cadmium- silymarin-training groups (p<0.001). Swimming training caused no significant changes in apoptotic index of hepatocytes (p=0.83). Conclusion: Swimming endurance training during pregnancy probably have no effect on maternal cadmium – induced of apoptosis in hepatocytes of neonates, however, silymarin could reduce it.

کلیدواژه‌ها [English]

  • Cadmium
  • Swimming training
  • Silymarin
  • apoptosis
  • Neonate liver
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