تاثیر 10 هفته تمرین هوازی تداومی و مکمل‌ سزامین بر سطوح سرمی اینترلوکین-6 و اینترلوکین-1 بتا در مردان تمرین کرده

نوع مقاله: مقاله پژوهشی

نویسندگان

1 استادیار گروه فیزیولوژی ورزشی و حرکات اصلاحی، دانشکده علوم ورزشی، دانشگاه ارومیه، ارومیه، ایران.

2 دانشجوی دکتری فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی و حرکات اصلاحی، دانشکده علوم ورزشی، دانشگاه ارومیه، ارومیه، ایران.

3 دانشیار گروه فیزیولوژی ورزشی و حرکات اصلاحی، دانشکده علوم ورزشی، دانشگاه ارومیه، ارومیه، ایران.

4 دانشیار گروه علوم ورزشی، دانشکده علوم تربیتی و روانشناسی، دانشگاه شهید مدنی آذربایجان، تبریز، ایران.

10.22077/jpsbs.2019.1199.1355

چکیده

زمینه و هدف: فعالیت ورزشی بسته به شدت و مدت تمرین، در اختلال یا بهبود سیستم ایمنی موثر است و استفاده از مکمل‌‌های گیاهی در کنار تمرین ورزشی، ترشح سایتوکاین‌های پیش و ضد التهابی را تنظیم کرده و بر سیستم ایمنی تاثیر مثبت دارد. هدف از پژوهش حاضر بررسی تاثیر 10 هفته تمرین هوازی تداومی و مکمل ‌‌سزامین بر سطوح سرمی اینتر‌لوکین-6 (IL-6) و اینتر‌لوکین-1 بتا (IL-1β) در مردان تمرین کرده بود. روش تحقیق: چهل آزمودنی مرد تمرین کرده (25 – 20 سال) به طور تصادفی در چهار گروه (هر گروه 10 نفر) شامل گروه دارونما، تمرین هوازی، تمرین هوازی + سزامین و سزامین قرار گرفتند. پروتکل تمرین هوازی هر جلسه 35-30 دقیقه، 4 روز در هفته و به مدت 10 هفته اجرا شد. مکمل سزامین به مقدار 50 میلی‌گرم در هر هفته به صورت کپسول ژلاتینی به گروه‌های دریافت کننده مکمل سزامین داده شد. همچنین، گروه دارونما کپسول‌ حاوی نشاسته مصرف نمود. اندازه گیری IL-6 و IL-1β با روش الایزا انجام شد. برای تحلیل داده‌ها از آزمون آماری تحلیل کوواریانس (ANCOVA) استفاده شد و سطح معنی‌داری 0/05>p در نظر گرفته شد. یافته‌ها: نتایج آزمون تحلیل کوواریانس نشان داد که پس از کنترل اثر پیش آزمون، تنها تمرین هوازی سبب کاهش معنی‌دار IL-6 (p=0/008، F=7/98) وIL-1β  (p=0/04، F=4/23) شد، اما اثر مکمل و اثر توام تمرین هوازی و مکمل بر این متغیرها، معنی‌دار نبود (0/05<p). نتیجه گیری: به نظر می رسد، در افراد تمرین کرده مصرف مکمل سزامین تاثیرات قابل توجهی بر سیستم ایمنی نداشته باشد. در مقابل، سازگاری ناشی از تمرین بهتر از مکمل سزامین در کنترل عوامل پیش التهابی نظیر IL-6 و IL-1β موثر است. با وجود این، به تحقیقات گسترده تر با بررسی سایر عوامل نیاز است تا به نتایج قطعی برسیم.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of 10 weeks of continuous aerobic training and Sesamin supplementation on serum levels of Interleukin-6 and Interleukin-1 Beta in trained men

نویسندگان [English]

  • Javad Tolouei Azar 1
  • Yousef Saberi 2
  • Asghar Tofigi 3
  • Bahloul Ghorbanian 4
1 Assistant Professor, Department of Exercise Physiology and Corrective Exercises, Faculty of Sport Sciences, Urmia University, Urmia, Iran.
2 PhD Student of Exercise Physiology, Department of Exercise Physiology and Corrective Exercises, Faculty of Sport Sciences, Urmia University, Urmia, Iran.
3 Associate Professor, Department of  Exercise Physiology and Corrective Exercises, Faculty of Sport Sciences, Urmia University, Urmia, Iran.
4 Associate Professor, Department of Sport Sciences, Faculty of Educational Sciences and Psychology, Azarbaijan Shahid Madani University, Tabriz, Iran.
چکیده [English]

Background and Aim: Physical activity depending on its intensity and duration could have an effective impact on disrupting or improving the immune system. Moreover, using the herbal supplement along with exercise, regulates the secretion of anti and pro-inflammatory cytokines, and has a positive impact on the immune system. The purpose of this study was to investigate the effect of 10 weeks of aerobic training and Sesamin supplementation on serum levels of Interleukin-6 (IL-6) and Interleukin-1 Beta (IL-1β) in trained men. Materials and Methods: 40 male subjects (20-25 years old) were randomly divided into 4 groups as placebo, aerobic training, aerobic training + Sesamin and Sesamin (n=10). The aerobic training protocol was performed as 30-35 minutes, 4 days a week for 10 weeks. Sesamin supplement in form of gelatin capsule was used by supplemented sesamin groups at dose of 50 mg per week. The placebo group also consumed capsules containing starch. Measurement of IL-6 and IL-1β were done using Elisa method. Data were analyzed using analysis of covariance (ANCOVA) at PResults: The results of ANCOVA test showed that after removing the effect of pre-test, only aerobic exercise showed significantly reduced the IL-6 (p=0.008, F=7.99) and IL-1β (p=0.04, F= 4.23). However, the effects of supplements and the interaction between exercise and supplementation did not indicate any significant change (P>0.05). Conclusion: It seems that taking Sesamin supplements does not have any significant effects on the immune system in trained men; while exercise-induced adaptations could modulate inflammatory factors (such as IL-6 and IL-1β) as compared to Sesamin supplementation. Totally, more extensive research with considering more factors are needed to clear results.

کلیدواژه‌ها [English]

  • Interleukin-6
  • Interleukin-1 Beta
  • Continouos aerobic training
  • Sesamin
  • Trained men
Accattato, F., Greco, M., Pullano, S. A., Carè, I., Fiorillo, A. S., Pujia, A., ... & Gulletta, E. (2017). Effects of acute physical exercise on oxidative stress and inflammatory status in young, sedentary obese subjects. PLoS One, 12(6), e0178900.

Ali Sholi, G. H., Ghanbarzadeh, M., Habibi, A., & Ranjbar, R. (2016). The effects of combined exercises intensity (aerobics-resistance) on plasma cortisol and testosterone levels in active males. International Journal of Basic Science in Medicine, 1(1), 18-24.

Baum, M., Klöpping-Menke, K., Müller-Steinhardt, M., Liesen, H., & Kirchner, H. (1999). Increased concentrations of interleukin 1-β in whole blood cultures supernatants after 12 weeks of moderate endurance exercise. European Journal of Applied Physiology and Occupational Physiology, 79(6), 500-503.

 Bean, S. (2018). Immune system response to high intensity exercise: Study 1: (bean) high intensity exercise increases cell death of B-Lymphocytes (CD19) in anaerobically irained individuals.  The Office of Research and Creative Activity. Western Kentucky University. Poster.

Chen, P. R., Chien, K. L., Su, T. C., Chang, C. J., Liu, T. L., Cheng, H., & Tsai, C. (2005). Dietary sesame reduces serum cholesterol and enhances antioxidant capacity in hypercholesterolemia. Nutrition Research, 25(6), 559-567.

Choi, E. J., & So, W. Y. (2018). The differential impact of high-intensity swimming exercise and inflammatory bowel disease on IL-1β, TNF-α, and COX-2 gene expression in the small intestine and colon in mice. Journal of Men's Health, 14(2), 22-29.

Chupel, M. U., Minuzzi, L. G., Furtado, G. E., Santos, M. L., Hogervorst, E., Filaire, E., & Teixeira, A. M. (2018). Exercise and taurine in inflammation, cognition, and peripheral markers of blood-brain barrier integrity in older women. Applied Physiology, Nutrition, and Metabolism, 43(7), 733-741.

Damirchi, A., Rahmani-Nia, F., & Mehrabani, J. (2011). Effect of a single bout graded exercise on the cytokines response and insulin resistance index. Brazilian Journal of Biomotricity, 5(2), 132-140.

Daneshmandi, H., & Daneshmandi, H. (2013). The effect of eight weeks of continuous exercise training and four weeks of workout on fat peroxidation and non-enzymatic antioxidant defense of passive men. Journal of Sport Biosciences, 6(3), 667-678. [Persian]

Donges, C. E., Duffield, R., & Drinkwater, E. J. (2010). Effect of resistance or aerobic exercise training on Interleukin-6, C-Reactive protein, and body. Medicine and Science in Sports and Exercise, 42, 304-313.

Farhadi, H., Rahimi, S., & Baghaei, B. (2013). The effect of eight weeks of supplementation of   pomegranate pills on inflammatory and muscular damage indices in non-athlete male subjects under the influence of different VO2max intensities. Journal of Practical studies of Biosciunces in Sport, 5(9), 31-41. [Persion]

Fielding, R. A., Frontera, W. R., Hughes, V. A., Fisher, E. C., & Evans, W. J. (1997). The reproducibility of the Bruce protocol exercise test for the determination of aerobic capacity in older women. Medicine and Science in Sports and Exercise, 29(8), 1109-1113.

Finaud, J., Scislowski, V., Lac, G., Durand, D., Vidalin, H., Robert, A., & Filaire, E. (2006). Antioxidant status and oxidative stress in professional rugby players: evolution throughout a season. International Journal of Sports Medicine, 27(02), 87-93.

Fleck, S. J., & Kraemer, W. (2014). Designing resistance training programs, 4th Edition. Human Kinetics.

Gleeson, M. (2007). Immune function in sport and exercise. Journal of Applied Physiology, 103(2), 693-699.

 Gleeson, M., Bishop, N. C., Stensel, D. J., Lindley, M. R., Mastana, S. S., & Nimmo, M. A. (2011). The anti-inflammatory effects of exercise: mechanisms and implications for the prevention and treatment of disease. Nature Reviews Immunology, 11(9), 607-615.

Goldhammer, E., Tanchilevitch, A., Maor, I., Beniamini, Y., Rosenschein, U., & Sagiv, M. (2005). Exercise training modulates cytokines activity in coronary heart disease patients. International Journal of Cardiology, 100(1), 93-99.

González Mosquera, D., Hernández Ortega, Y., Fernández, P., González, Y., Doens, D., Vander Heyden, Y., . . . & Pieters, L. (2018). Flavonoids from Boldoa purpurascens inhibit proinflammatory cytokines (TNF‐α and IL‐6) and the expression of COX‐2. Phytotherapy Research, 32(9), 1750-1754.

Haghighian, M. K., Alipoor, B., Mahdavi, A. M., Sadat, B. E., Jafarabadi, M. A., & Moghaddam, A. (2015). Effects of sesame seed supplementation on inflammatory factors and oxidative stress biomarkers in patients with knee osteoarthritis. Acta Medica Iranica, 53(4), 207-213. [Persian]

Ibrahiem, T. A. (2016). Beneficial effects of diet supplementation with Nigella sativa (Black Seed) and sesame seeds in Alloxan-Diabetic Rats. International Journal of Current Microbiology and Applied Sciences, 5, 411-423.

Jordan, S. L. (2007). The effects of green tea extract supplementation on delayed onset muscle soreness and oxidative stress. Ph.D Thesis, Texas Tech University.

Kasapis, C., & Thompson, P. D. (2005). The effects of physical activity on serum C-reactive protein and inflammatory markers: a systematic review. Journal of the American College of Cardiology, 45(10), 1563-1569.

Kohut, M. L., McCann, D. A., Russell, D. W., Konopka, D. N., Cunnick, J. E., Franke, W. D., ... & Vanderah, E. (2006). Aerobic exercise, but not flexibility/resistance exercise, reduces serum IL-18, CRP, and IL-6 independent of β-blockers, BMI, and psychosocial factors in older adults. Brain, Behavior, and Immunity, 20(3), 201-209.

Lesslie, M. (2017). Investigations of biologically relevant free radicals utilizing novel gas-phase analytical techniques. PhD Thesis, Northern Illinois University.

Malekyian Fini, E., Shavandi, N., & Saremi, A. (2013). Effect of short-term Resvin supplementation on total antioxidant capacity ‚super oxide dismutase, and creatine kinase in elite women volleyball players. Iranian Journal of Nutrition Sciences & Food Technology, 8(3), 79-86. [Persian]

Martín, A. R., Villegas, I., Sánchez‐Hidalgo, M., & De La Lastra, C. A. (2006). The effects of resveratrol, a phytoalexin derived from red wines, on chronic inflammation induced in an experimentally induced colitis model. British Journal of Pharmacology, 147(8), 873-885.

Namazi, A., Aghaalinejad, H., Peeri, M., & Rahbarizadeh, F. (2010). The effects of short term circuit resistance training on serum homocysteine and CRP concentrations in active and inactive females. Iranian Journal of Endocrinology and Metabolism, 12(2), 169-201. [Persian]

Nieman, D. C., Zwetsloot, K. A., Meaney, M. P., Lomiwes, D. D., Hurst, S. M., & Hurst, R. D. (2015). Post-exercise skeletal muscle glycogen related to plasma cytokines and muscle IL-6 protein content, but not muscle cytokine mRNA expression. Frontiers in Nutrition, 2, 27.

Paolucci, E. M., Loukov, D., Bowdish, D. M., & Heisz, J. J. (2018). Exercise reduces depression and inflammation but intensity matters. Biological Psychology, 133(12), 79-84.

Peake, J., Della Gatta, P., Suzuki, K., & Nieman, D. (2015). Cytokine expression and secretion by skeletal muscle cells: regulatory mechanisms and exercise effects. Exercise Immunology Review, 21, 8-25.

Petersen A. M. W., & Pedersen, B. K. (2005). The anti-inflammatory effect of exercise. Journal of Applied Physiology, 98(4), 1154-62.

Peterson, J., Dwyer, J., Adlercreutz, H., Scalbert, A., Jacques, P., & McCullough, M. L. (2010). Dietary lignans: physiology and potential for cardiovascular disease risk reduction. Nutrition Reviews, 68(10), 571-603.

Rapoport, B. I. (2010). Metabolic factors limiting performance in marathon runners. PLOS Computational Biology, 6(10), 1-13.

Schirmer, M., Kumar, V., Netea, M. G., & Xavier, R. J. (2018). The causes and consequences of variation in human cytokine production in health. Current Opinion in Immunology, 54, 50-58.

Sharifian, A., Farahani, S., Pasalar, P., Gharavi, M., & Aminian, O. (2005). Shift work as an oxidative stressor. Journal of Circadian Rhythms, 3(1), 15-22.

Sheikholeslami, V. D., Ahmadi, S., Mojtahedi, H., Marandi, M., Ahmadi, D. K., Faraji, H., & Gharibi, F. (2011). Influence of different intensities of resistance exercise on inflammatory markers in young healthy men. Iranian Journal of Endocrinology and Metabolism, 12(6), 618-625.  [Persian]

Sibrian-Vazquez, M., Escobedo, J. O., Lim, S., Samoei, G. K., & Strongin, R. M. (2010). Homocystamides promote free-radical and oxidative damage to proteins. Proceedings of the National Academy of Sciences, 107(2), 551-554.

Stensvold, D., Slørdahl, S. A., & Wisløff, U. (2012). Effect of exercise training on inflammation status among people with metabolic syndrome. Metabolic Syndrome and Related Disorders, 10(4), 267-272.

Ünal, M. K., & Yalçın, H. (2008). Proximate composition of Turkish sesame seeds and characterization of their oils. Grasas y Aceites, 59(1), 23-26.

Wagenmakers, A. J., & Pedersen, B. K. (2006). The anti-inflammatory effect of exercise: its role in diabetes and cardiovascular disease control. Essays in Biochemistry, 42, 105-117.

Wong, P. C., Chia, M., Tsou, I. Y., Wansaicheong, G. K., Tan, B., Wang, J. C., ... & Lim, D. (2008). Effects of a 12-week exercise training programme on aerobic fitness, body composition, blood lipids and C-reactive protein in adolescents with obesity. Annals of the Academy of Medicine, 37(4), 86-93.

Wu, J. H., Hodgson, J. M., Puddey, I. B., Belski, R., Burke, V., & Croft, K. D. (2009). Sesame supplementation does not improve cardiovascular disease risk markers in overweight men and women. Nutrition, Metabolism and Cardiovascular Diseases, 19(11), 774-780.