تاثیر یک دوره تمرین هوازی اجباری در دوران نوجوانی بر اثرات منفی استرس جدایی از مادر بر استرس اکسیداتیو قلبی در رت های نر بالغ

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دکترای فیزیولوژی ورزش، دانشگاه آزاد اسلامی، واحد تهران مرکز، تهران، ایران.

2 استاد، گروه فیزیولوژی ورزش، دانشگاه آزاد اسلامی، واحد تهران مرکز، تهران، ایران.

چکیده

زمینه و هدف: استرس زودرس به عنوان عامل خطر توسعه افسردگی و آسیب­های مرتبط با آن شناخته شده­است. کاردیومیوپاتی نقش مهمی در پاتوفیزیولوژی افسردگی و بیماری­های قلبی - عروقی بازی می­کند. شواهد نشان می­دهد که فعالیت بدنی منظم در دوران نوجوانی ممکن است بتواند اثرات منفی استرس جدایی از مادر را به عنوان یک مدل حیوانی معتبر افسردگی بر رفتار و عملکرد میتوکندریایی قلبی رت­های بالغ تعدیل کند. روش تحقیق: در این مطالعۀ تجربی، استرس جدایی از مادر در 72 نوزاد رت صحرایی نر بوسیلۀ جدایی آن ها از مادرانشان برای 180 دقیقه در روز پس از زایمان روز دوم تا چهاردهم اعمال شد. سپس حیوانات به طور تصادفی در گروه­های مختلف دوازده تایی (فلوکستین و نوارگردان) تقسیم شدند و معالجات را طی دوران بلوغ دریافت نمودند. سرانجام اثرات استرس جدایی از مادر در آزمون­های رفتاری رت­ها ارزیابی شد. نهایتاً، گونه­های اکسیژن واکنشی، گلوتاتیون میتوکندریایی، ATP و رهایش سیتوکروم c در بافت قلبی حیوانات اندازه گیری گردید. با استفاده از آزمون t مستقل و تحلیل واریانس یک طرفه و در سطح معنی داری 05/0≥p؛ داده ها تجزیه و تحلیل شدند. یافته­ها: رفتارهای شبه افسردگی بدنبال استرس جدایی از مادر در رت­های صحرایی نر بالغ با اختلال عملکرد میتوکندری قلبی در حیوانات همراه بود. علاوه بر این، نتایج نشان داد که درمان حیوانات با فلوکستین و تمرین اجباری روی نوارگردان طی دوره نوجوانی برخی رفتارهای حیوانی و عملکردهای میتوکندری قلبی را بهبود می­بخشد. نتیجه­گیری: نتایج این مطالعه قویاً بیانگر اهمیت نوجوانی به عنوان دوره­ای است که طی آن درمان حیوانات با اجرای تمرین نوارگردان، اثرات محافظتی قابل توجهی بر اختلالات هموستاز انرژی قلبی ناشی از استرس جدایی از مادر دارد.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of mandatory aerobic exercise in adolescence on the negative impact of maternal separation stress on cardiac oxidative stress in adult male rat

نویسندگان [English]

  • Ehtram alsadat Sahafi 1
  • Maghsoud Peeri 2
1 PhD of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
2 Full Professor, Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
چکیده [English]

Background and Aim: Early life stress is known as a risk factor for the development of depression and its associated comorbidities. Mitochondrial dysfunction plays a critical role in the pathophysiology of depression and cardiovascular diseases. Evidence indicated that regular physical activity during adolescence may be able to adjust the negative impact of maternal separation stress as a valid animal model of depression on behavior and cardiac mitochondrial function of adult rats. Materials and Methods: In this experimental study, maternal separation stress was applied to 72 male rat pups by separating them from their mothers for 180 minutes during second to fourteenth day of postnatal. Then, the animals were randomly divided into different treatment six groups (fluoxetine and treadmill) and received the treatments during adolescence. Further, we evaluated the effects of maternal separation stress on the rat behaviors test. Finally, we assessed the reactive oxygen species, mitochondrial glutathione, ATP and cytochrome c release in the cardiac tissue of animals. Comparison between groups were analyzed using independent t-test and one-way analysis of variance and statistically significant was considered if p≤0.05. Result: The results of this study showed tha depressive-like behaviors following maternal separation stress in adult male rats were associated with oxidative stress in cardiac tissue. Also the results showed that treating animals with fluoxetine or compulsory exercise on the treadmill during adolescence improved some cardiac mitochondrial function. Conclusion: The results of this study highlights the importance of adolescence as a period during which treating animals by performing a treadmill exercise has significant protective effects on cardiac energy homeostasis disorders caused by maternal separation stress.

کلیدواژه‌ها [English]

  • Maternal separation stress
  • Cardiomiopaty
  • mandatory aerobic exercise
  • Reactive oxygen species
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