تاثیر ۸ هفته تمرین استقامتی بر بیان ژن امنتین-1 بافت چربی احشایی رت های نر دیابتی شده با استرپتوزوتوسین

نوع مقاله: مقاله پژوهشی

نویسندگان

1 کارشناس ارشد فیزیولوژی ورزشی، دانشگاه پیام نور، تهران، ایران.

2 دانشیار گروه فیزیولوژی ورزش، دانشگاه پیام نور، تهران، ایران.

3 استادیار گروه فیزیولوژی ورزش، دانشگاه پیام نور، تهران، ایران.

چکیده

زمینه و هدف: دیابت یکی از معضلات سلامتی در تمامی جوامع محسوب می‌ شود و مداخله ورزشی از جمله رویکردهای بهبود وضعیت افراد دیابتی تلقی می‌گردد. هدف از تحقیق حاضر بررسی ‌تأثیر تمرین استقامتی بر بیان ژن امنتین-۱ بافت چربی احشایی در موش‌های نر دیابتی نژاد ویستار بود. روش تحقیق: از بین 19 سر رت نر نژاد ویستار، به صورت تصادفی ۶ سر رت به عنوان گروه کنترل پایه انتخاب شدند. دیابت بر رت های باقی مانده از طریق رژیم غذایی پر چرب و تزریق درون صفاقی استرپتوزوتوسین القا گردید. سپس رت ها  ‌به طور تصادفی به دو گروه دیابتی تمرین استقامتی (6 سر) و کنترل دیابتی (7 سر) تقسیم شدند. تمرین استقامتی شامل دویدن روی نوارگردان با سرعت 50 تا 70 درصد VO2max و با رعایت اصل اضافه بار 5 روز در هفته به مدت 8 هفته انجام گرفت. پس از پایان ۸ هفته تمرین، از طریق استخراج بافت چربی احشایی، نمونه ها جمع‌آوری گردید. اندازه گیری بیان ژن امنتین -1 با روش RT-PCR انجام شد. تجزیه و تحلیل داده ها با نرم افزار SPSS از طریق آزمون تحلیل واریانس یک طرفه و آزمون تعقیبی توکی در سطح معنی داری 0/05>p انجام گرفت. یافته ها:  تفاوت معنی داری در وزن موش ها در حالت پایه و پس از 8 هفته تمرین استقامتی وجود نداشت (0/05<p). اما 8 هفته تمرین استقامتی تداومی در رت های دیابتی شده باعث افزایش معنی‌دار بیان ژن امنتین-۱ بافت چربی احشایی شد (0/001<‌p). نتیجه گیری: با توجه به تاثیر تمرین استقامتی بر افزایش بیان ژن امنتین -1 و نقش  این عامل در فعال نمودن مسیر AKT و افزایش برداشت گلوکز توسط بافت چربی‌، احتمال می‌رود افزایش بیان این آدیپوکاین نقش مهمی در کاهش قند خون  بیماران دیابتی داشته باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of 8 weeks endurance training on omentin-1 gene expression of visceral adipose tissue in Streptozotocin-induced diabetic male rats

نویسندگان [English]

  • Mahdieh Alizadeh 1
  • Mohammadreza Asad 2
  • saeed naghibi 3
1 MS.c in Exercise Physiology, Alborz Payame Noor University, Tehran, Iran.
2 Associate Professor, Department of Exercise physiology, Alborz Payame Noor University, Tehran, Iran.
3 Assistant Professor, Tehran Payame Noor University, Tehran, Iran.
چکیده [English]

Background and Aim: Diabetes is a health problem in all societies, and exercise training is one of the best method to control blood glucose levels. The aim of this study was to investigate the effect of 8 weeks of endurance training on omentin-1 gene expression of visceral adipose tissue in diabetic male rats. Materials and Methods: Among 19 male rats 6 mice were randomly selected as controls basic group. The remaining rats were given a high-fat diet and free access to food and water and then diabetes was induced by streptozotocin. Diabetic rats were divided into two groups including endurance training (n=6) and control (n=7) groups. Endurance training exercised on a treadmill up to 50 to 70 percent of maximal oxygen uptake, 5 days per week for 8 weeks based on the overload pattern. After 8 weeks, samples of visceral adipose tissue were collected and omentin-1 gene expression determined using RT-PCR method. It is applied the ANOVA and Tukey post hoc tests for extracted of results using SPSS software at the significant level of pResults: There were no significant differences in the weight of rats in baseline and after 8 weeks of endurance training (p>0.05). However, omentin-1 gene expression significantly increased after continuous endurance training (p=0.001). Conclusion: Based on omentin-1 gene expression improvement and its role to activation of AKT pathway and glucose uptake elevation by adipose tissue, it seems that this adipokine have an important effect in lowering blood sugar in diabetic patients.

کلیدواژه‌ها [English]

  • Endurance training
  • Omentin-1 gene
  • Visceral adipose tissue
  • Diabetes type II
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