تأثیر یک دوره تمرین تناوبی همراه با مکمل پرسیاوشان بر سطوح پروتئین سرفکتانت (SP-A) ریه رت‌های نر

نوع مقاله: مقاله پژوهشی

نویسندگان

1 کارشناس ارشد فیزیولوژی ورزش، دانشکده علوم ورزشی، دانشگاه مازندران، بابلسر، ایران.

2 استاد گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی،دانشگاه مازندران، بابلسر، ایران.

3 دانشیار، پژوهشکدة علوم غدد و متابولیسم، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران.

چکیده

زمینه و هدف: SP-A  فراوان ترین پروتئین سرفکتانت ریوی است که نقش مهمی در از بین بردن عفونت و از بین بردن واسطه های التهابی دارد. از طرفی، پرسیاوشان با دارا بودن عناصر ضدالتهابی همچون فلاونوئیدها و ساپونین ها، نقش مؤثری در مهار واکنش های التهابی بازی می کند. هدف تحقیق حاضر بررسی تأثیر یک دوره تمرین تناوبی همراه با مکمل پرسیاوشان برسطوح  SP-Aریه رت­های نر بود. روش تحقیق: در این مطالعة تجربی، 30 سر رت نر نژاد ویستار سه ­هفته­ای با میانگین وزن 9±68 گرم به طور تصادفی به گروه های کنترل و تمرین تقسیم شدند. گروه تمرین پس از پایان دورة 6 هفته ای تمرین تناوبی، به سه گروه تمرین، مکمل و تمرین + مکمل تقسیم گردید. به گروه های مکمل و مکمل+ تمرین، به مدت سه هفته مکمل گیاهی پرسیاوشان به صورت روزانه 200 میلی گرم/کیلوگرم وزن بدن به صورت گاواژ خورانده ­شد. اندازه گیری سطوح SP-A ریه با استفاده از کیت  کازابایو ساخت کشور چین و به روش الایزا انجام شد. تحلیل داده ها با آزمون تحلیل واریانس یک طرفه و آزمون تعقیبی LSD در سطح 05/0≥p انجام گرفت. یافته ها: پس از مدت 6 و 9 هفته تمرین تناوبی نسبت به گروه های کنترل، تفاوت معنی داری در سطوح SP-A ریه (به ترتیب با 14/0p= و 28/0p=) مشاهده نگردید؛ اما سطوح  SP-A ریه در گروه تمرین – مکمل پرسیاوشان نسبت به سایر گروه ها  (0001/0p=) به طور معنی داری کاهش یافت. نتیجه گیری: به نظر می رسد مکمل پرسیاوشان با توجه به ترکیبات ضد اکسایشی موجود در آن توانسته است اثرات پاتوفیزیولوژیکی تمرین تناوبی فزآینده را مهار و شاخص های التهابی را به خوبی کاهش دهد و موجب تقویت سیستم ایمنی ریه شود؛ هر چند بررسی های بیشتر در این زمینه ضرورت دارد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of interval training along with Adiantum capillus-veneris Linn supplemen on surfactant protein (SP-A) in lung male rats

نویسندگان [English]

  • Seyed Hadi Hosseini 1
  • Shadmehr Mirdar Harijani 2
  • Mehdi Hedayati 3
1 MSc Student in Exercise Physiology, University of Mazandaran, Babolsar, Iran.
2 Full Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Mazandaran, Babolsar, Iran.
3 Associate Professor, Cellular and Molecular Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
چکیده [English]

Background and Aim: Surfactant protein (SP-A) is most abundant surfactant protein in lungs and plays an important role in eliminating the infection and inflammatory mediators. Further, Adiantum capillus-veneris Linn (Pare-siavashan) composed elements such as flavonoids and saponins maidenhair and may play an effective role in inhibiting the inflammatory responses. The aim of this study was to investigate the effect of interval training along with Adiantum capillus-veneris Linn supplementation on levels of SP-A in lung male rats. Materials and Methods: Thirty male Wister rats with three weeks old and average weight of 68 ± 9 g were randomly divided into 2 groups including interval training and control groups. The interval training group was divided into three sub-groups including training, supplementation and training+supplementation groups after the end of 6-week interval training. The Adiantum capillus-veneris Linn herbal supplement were fed orally for 3 weeks, with the daily dose of 200 mg per kilogram of body weight. The levels of lung SP-A was measured using Cusabio ELISA kit (China Company). The data were analyzed using one-way ANOVA and LSD tests at the significant level of p≤0.05. Results: The results showed no significant differences between lung SP-A levels after 6 (p=0.28) and 9 (p=0.14) weeks of interval training compared to control group; however SP-A levels significantly decreased in the training - Adiantum capillus-veneris Linn group (p=0.001) compared to other interventions. Conclusion: Regarding the significant reduction of SP-A after interval training, it seems that supplementation may inhibit the pathophysiological effects of incremental trainings and to reduce the inflammatory parameters well and to strengthen the lung immunity.

کلیدواژه‌ها [English]

  • Interval training
  • Herbal supplementation
  • Adiantum capillus-veneris Linn
  • Lung surfactant
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