تاثیر تمرین مقاومتی بر قدرت عضلانی، هایپرتروفی و سطوح پروتئین میوژنین عضلات دوقلو در موش های صحرایی سالمند

نوع مقاله: مقاله پژوهشی

نویسندگان

استادیار گروه علوم ورزشی، دانشکده علوم ورزشی، دانشگاه شمال، آمل، ایران.

چکیده

زمینه و هدف: تمرین مقاومتی (RT) یکی از موثرترین استراتژی‌ها برای پیشگیری از تضعیف و تخریب عضلانی ناشی از سن است؛ زیرا قدرت و عملکرد عضلانی را بهبود می بخشد. از آنجایی که کاهش توده عضلانی در ایجاد سارکوپنی مشارکت دارد، اثر تمرین مقاومتی بر هایپرتروفی عضلانی و فرآیندهای میوژنیک آن در سالمندی مهم و بحث‌برانگیز است. هدف از این تحقیق بررسی اثر تمرین مقاومتی بر قدرت، توده عضلانی و سطوح پروتئین میوژنین در عضلات دوقلوی موش‌های سالمند بود. روش تحقیق: 16 سر موش صحرایی نر سالمند (24 ماهه) به طور مساوی به دو گروه تمرین مقاومتی و کنترل تقسیم شدند. گروه تمرین مقاومتی تحت هشت هفته تمرین مقاومتی پیشرونده (3 جلسه در هفته) بالارفتن از نردبان با وزنه بسته‌شده به دم قرار گرفت. 48 ساعت پس از آخرین جلسه، نیروی ایزومتریک، وزن عضله و سطوح پروتئین میوژنین عضلهدوقلو هر دو گروه اندازه‌گیری شد. برای تجزیه و تحلیل از آزمون t مستقل در سطح معنی­داری 05/0p< استفاده شد.  یافته ها: نیروی مطلق و نسبی عضله (نسبت به وزن بدن) در گروه تمرین مقاومتی به طور معنی‌داری بیش از کنترل بود (05/0p˂). وزن تر دوقلو بین دو گروه تفاوت معنی داری نداشت (05/0p>). تحلیل وسترن‌بلات بافت عضلانی نشان داد  که سطوح پروتئین میوژنین تفاوت معنی‌داری بین دو گروه ندارد (05/0p>). نتیجه‌گیری: به دنبال تمرین مقاومتی در موش‌های سالمند ظرفیت تولید نیرو و کیفیت عضلانی (نیرو نسبت به توده عضلانی) از طریق رخدادهایی که به نظر می‌رسد ناشی از درگیری سازگاری‌های عصبی- عضلانی باشد افزایش می‌یابد. علاوه بر این یافته‌ها پیشنهاد می کنند که افزایش در قدرت عضلانی پس از تمرین مقاومتی در موش‌های سالمند به وسیله تغییرات توده عضلانی و بیان پروتئین میوژنین قابل توجیه نیست.

کلیدواژه‌ها


عنوان مقاله [English]

The effects of resistance training on muscle strength, hypertrophy and myogenin protein level of gastrocnemius in elderly rats

نویسندگان [English]

  • Taher Afsharnezhad Roudsari
  • AliReza Amani
Assistant Professor, Department of Sport Sciences, Faculty of Sport Sciences, Shomal University, Amol, Iran.
چکیده [English]

Background and Aim: Resistance training (RT) is the most effective strategy to prevent age-related muscle wasting and weakness, because it promotes muscle strength and function. As the loss of muscle mass contributes to sarcopenia, the effects of RT on hypertrophy and its myogenic processes is controversial in old age. The purpose of this study was to examine the effects of RT on strength, mass and protein level of myogenin in gastrocnemius muscle of elderly rats. Materials and Methods: Sixteen elderly male Sprague-Dawley rats (24-month age) divided equally to two groups (control and RT). RT group underwent 8weeks (3-days/week) of resistance training by climbing a wooden ladder with weights attached to their tails. 48h after last session, isometric force, muscle wet mass and protein level of myogenin of gastrocnemius muscle were measured in both groups. For statistical analysis, independent sample t-test was used with a significance level of 0.05. Results: Absolute and relative (to body mass) isometric force of RT group were significantly greater than those in control group. There was not any significant difference in wet muscle mass between groups. Western blot analysis of muscle tissue also showed that the levels of myogenin did not significantly differ between two groups. Conclusion: Force production capacity and muscle quality (force to muscle mass ratio) were increased following resistance training in elderly rats through events are likely caused by neuromuscular adaptations. Additionally, the results suggest that increase in strength after resistance training in aged rats cannot be explained in terms of the changing in muscle mass and myogenin expression values.

کلیدواژه‌ها [English]

  • resistance training
  • Hypertrophy
  • Muscle strength
  • Myogenin
  • Aging

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