تأثیر دویدن روی نوارگردان با شدت کم بر بیان ژن های PGC-1α، FNDC5 و BDNF درهیپوکامپ موش های صحرایی نر

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی، گروه تربیت بدنی و علوم ورزشی، دانشکده علوم انسانی، دانشگاه تربیت مدرس تهران، ایران.

2 استاد فیزیولوژی ورزشی، گروه تربیت بدنی و علوم ورزشی، دانشکده علوم انسانی، دانشگاه تربیت مدرس تهران، ایران

3 استاد فیزیولوژی، گروه فیزولوژی و فارماکولوژی، موسسه پاستور، تهران ، ایران

4 دانشجوی دکتری فیزیولوژی ورزشی، گروه تربیت بدنی و علوم ورزشی، دانشکده علوم انسانی، دانشگاه تربیت مدرس تهران، ایران

5 دانشجوی دکتری فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه خوارزمی تهران، ایران

چکیده

زمینه و هدف: مطالعات پیشین نشان داده­­ اند که عامل نوروتروفیک مشتق شده از مغز (BDNF) در القاء اثرات مفید فعالیت ورزشی بر مغز به ویژه هیپوکامپ نقش بسیار حیاتی دارد. با این وجود مسیرهای پیام­ دهی درگیر در افزایش BDNF ناشی از تمرین ورزشی اجباری در هیپوکامپ به خوبی شناخته نشده است. از این رو، هدف از مطالعه حاضر بررسی تأثیر هشت هفته تمرین اجباری نوارگردان با شدت کم بر بیان ژن ­های گیرنده فعال کننده تکثیر پروکسی زوم گاما هم ­فعال ساز آلفا (PGC-1α)، فیبرونکتین نوع 3 حاوی پروتئین 5 (FNDC5) و BDNFدر هیپوکامپ موش­ های صحرایی نر بود. روش تحقیق: تعداد 18 سر موش صحرائی نر نژاد ویستار به طور تصادفی به 3 گروه کنترل (6 سر)، شم (6 سر) و تمرین اجباری (6 سر) تقسیم شدند. حیوانات در گروه تمرین ورزشی به انجام 8 هفته تمرین اجباری (5 جلسه در هفته) با شدت پایین (سرعت 15 متر بر دقیقه) بر روی نوارگردان پرداختند. 24  ساعت پس از آخرین جلسة تمرینی همه موش ­ها بی هوش شدند. سر حیوانات جدا شد و هیپوکامپ استخراج گردید و در دمای 80- درجة سانتی­گراد برای تجزیه تحلیل­ های بعدی نگهداری شد. به منظور سنجش میزان بیان ژن­ ها در هیپوکامپ از روش واکنش زنجیره پلی­مراز زمان واقعی (Real-Time-PCR) استفاده شد. داده­ ها با روش تحلیل واریانس یکطرفه و آزمون تعقیبی توکی در سطح 05/0>p تجزیه تحلیل شد. یافته­ ها: نتایج این تحقیق نشان داد سطوح ژن­ های PGC-1α (003/0P<)، FNDC5   (006/0P<) و  BDNF (02/0P<) در گروه تمرین اجباری نسبت به گروه کنترل به طور معنی­ داری بالاتر بود. اما تفاوتی بین گروه شم و کنترل در میزان mRNA ژن­ها مشاهده نشد. نتیجه ­گیری: به نظر می­رسد تمرین اجباری با شدت کم احتمالاً از مسیر پیام ­دهی وابسته به  PGC-1αمنجر به افزایش بیان FNDC5 و در نتیجه افزایش بیان BDNF می­گردد. بنابراین، این نوع از تمرین ورزشی را می­ توان به منظور القاء اثرات مفید ورزش بر هیپوکامپ، به کار گرفت.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of the treadmill running on genes expression ofthePGC-1α, FNDC5 and BDNF in hippocampus of male rats

نویسندگان [English]

  • Seyyed Mohammad Ali Azimi Dokht 1
  • Reaz Gharakhanlou 2
  • Naser Naghdi 3
  • Davar Khodadadi 4
  • Ali Asghar Zare Zade Mehrizi 5
1 PhD Student of Exercise Physiology, Department of Physical Education and Sport Sciences, Faculty of Humanities, TarbiatModares University, Tehran, Iran.
2 Professor, Department of Physical Education and Sport Sciences, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran
3 Professor, Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran
4 PhD Student of Exercise Physiology, Department of Physical Education and Sport Sciences, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran
5 PhD Student of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Kharazmi University, Tehran, Iran
چکیده [English]

Background and Aim: Previous studies have shown that brain-derived neurotrophic factor (BDNF) plays a vital role to induce the beneficial effects of exercise on the brain, especially the hippocampus. However, signaling pathways related to increasing BDNF induced by forced exercise in hippocampus not well known. Therefore, the purpose of current study was to investigate the effect of 8-weeks of low-intensity forced treadmill training on genes expression of peroxisome proliferator activated receptor-gamma co-activator 1-alpha (PGC-1α), Fibronectin type III domain containing 5 (FNDC5) and BDNF in hippocampus of male rats. Materials and Methods: Eighteen male Wistar rats were randomly divided into 3 groups: Control (n=6), Sham (n=6) and Forced training (n=6). Animals in the training group performed 8 weeks of forced training (5 sessions per week) with low-intensity (speed: 15 m/min) on the treadmill. Twenty-four hours after last session of exercise, rats were decapitated and the hippocampus were carefully removed and rapidly frozen in liquid nitrogen, and finally stored at -80°C for further analysis. Real-Time-PCR method was used to measure the expression of genes in the hippocampus. Data were analyzed by one way ANOVA and Tukey post hoc test at the significant level of p<0.05.Results: The results of showed that mRNA levels of PGC-1α(p<0.003), FNDC5 (p<0.006) and BDNF(p<0.02) in the forced training group were significantly higher than the control group. However, there was no significant difference in the mRNA levels of genes between the sham and control groups. Conclusion: It seems that the low-intensity forced training, likely through a PGC-1α-dependent signaling pathway, leads to increasing expression of FNDC5 and as a result causes increasing the expression of BDNF. Thus, this type exercise training can be used as induction of beneficial effects of exercise on the hippocampus.

کلیدواژه‌ها [English]

  • low-intensityforced Training
  • PGC-1α
  • FNDC5
  • BDNF
  • Rat Hippocampus

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