اثر تعاملی مصرف ویتامین E همراه با تمرینات ورزشی تداومی و تناوبی بر محتوای مغزی عامل رشد اندوتلیال عروقی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 استادیار فیزیولوژی ورزشی، گروه علوم ورزشی، دانشگاه بجنورد، بجنورد، ایران.

2 دانشیار بیوشیمی بالینی، گروه بیوشیمی بالینی، دانشگاه علوم پزشکی گناباد، گناباد، ایران.

3 استادیار فیزیولوژی ورزشی، مرکز تحقیقات فیزیولوژی ورزش، دانشگاه علوم پزشکی بقیه الله، تهران، ایران.

4 استادیار فیزیولوژی ورزشی، گروه علوم ورزشی، واحد ایلام، دانشگاه آزاد اسلامی، ایلام، ایران.

چکیده

زمینه و هدف: پراکسید هیدروژن (2O2H) باعث بیان عامل رشد اندوتلیال عروقی (VEGF) می‌شود، این در حالی است که  این دو عامل خود تحت تاثیر تمرینات ورزشی و ویتامین E قرار می‌گیرند. هدف مطالعه حاضر بررسی اثر مصرف ویتامین E سوکسینات و تمرینات تداومی و تناوبی برVEGF و 2O2H مغز موش‌های صحرایی بود. روش تحقیق: 56 سر موش‌ صحرایی آلبینو ویستار (12 هفته سن، 250 تا 300 گرم وزن) به طور تصادفی در 7 گروه کنترل (C)، حامل (V)، مکمل (S)، تمرین تداومی (CT)، تمرین تداومی + مصرف مکمل (CT+S)، تمرین تناوبی (IT) و تمرین تناوبی + مصرف مکمل (IT+S) تقسیم شدند. مغز موش‌ها متعاقب 6 هفته تمرین تداومی (80 درصد max2VO) و تناوبی (95 تا 100 درصد max2VO) توام با مصرف ویتامین E (60 میلی‌گرم/کیلوگرم وزن بدن/روز) برداشته شد. محتوای VEGF  به روش ساندویچ الایزا و 2O2H به روش رنگ‌ سنجی اندازه‌گیری شدند. از آزمون تحلیل واریانس یک طرفه و آزمون تعقیبی بونفرونی برای استخراج نتایج در سطح  0/05>p استفاده شد. یافته‌ها: سطوح 2O2H (به ترتیب با 0/01=p و 0/001=p) وVEGF  (به ترتیب با 0/007=p و 0/001=p) مغز به طور معنی‌داری در هر دو گروه CT  و IT نسبت به گروه C افزایش یافت؛ به گونـه‌ای که افزایش 2O2H وVEGF  در گروه IT نسبت به CT  بیشتر بود (0/02=p برای هر دو عامل 2O2H  وVEGF). با وجود این، مکمل‌دهی ویتامین E تاثیری بر 2O2H (به ترتیب با 0/59=p= 0/99 ،p و 0/51=p) و VEGF (به ترتیب با 0/99=p= 0/99 ،p و 0/67=p) مغز سه گروه S،CT+S  وIT+S  نداشت. به علاوه، همبستگی معنی‌داری بین 2O2H و VEGF مغز دیده شد (0/001=p= 0/73 ،r). نتیجه‌گیری: اجرای تمرینات ورزشی تناوبی با حداکثر شدت نسبت به اجرای تداومی، موجب افزایش بیشتری در عوامل درگیر در رگ زایی می‌شود و مکمل‌دهی ویتامین E سوکسینات تاثیری بر این سازگاری‌های ندارد.

کلیدواژه‌ها


عنوان مقاله [English]

The interactive effect of vitamin E supplementation along with continuous and interval exercise trainings on brain content of vascular endothelial growth factor

نویسندگان [English]

  • Hossein TaheriChadorneshin 1
  • Seyed-Hosein Abtahi-Eivary 2
  • Hossein Shirvani 3
  • Mohammad-Reza Yousefi 4
1 Assistant Professor of Exercise Physiology, Department of Sport Sciences, University of Bojnord, Bojnord, Iran.
2 Associate Professor of Clinical Biochemistry, Department of Clinical Biochemistry, Gonabad University of Medical Sciences, Gonabad, Iran.
3 Assistant Professor of Exercise Physiology, Exercise Physiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
4 Assistant Professor of Exercise Physiology, Department of Sport Sciences, Ilam Branch, Islamic Azad University, Ilam, Iran.
چکیده [English]

Background and Aim: Hydrogen peroxide (H2O2) increases the vascular endothelial growth factor (VEGF), while these two factores are affected by exercise and vitamin E. The aim of the present study was to investigate the effect of vitamin E succinate supplementation along with continuous and interval exercise trainings on VEGF and H2O2 in rat brain. Material and Methods: Fifty-six Albino Wistar rats (12 years old, 250 to 300 gr) were randomly divided into 7 groups: control (C), vehicle (V), supplements (S), continuous training (CT), continuous training + supplement (CT+S), interval training (IT), interval training + supplementation (IT+S). Rat brain was dissected after 6 weeks of continuous (80% VO2max) and interval exercise training (95 to 100% VO2max) along with vitamin E supplementation (60 mg/kg body weight/day). The content of VEGF and H2O2 were measured using sandwich ELISA and colorimetric assay, respectively. Data were analysed using one-way analysis of variance followed by Bonferroni post-hoc comparison at pResults: Brain H2O2 (p=0.01 and p=0.001, respectively) level and VEGF (p=0.007 and p=0.001, respectively) increased significantly in both of CT and IT groups compare to C group; while the IT resulted in a greater increase in H2O2 and VEGF than those of CT (p=0.02 for both of H2O2 and VEGF). However, vitamin E supplementation had no significant effect on H2O2 (p=0.59, p=0.99 and p=0.51, respectively) and brain VEGF (p=0.99, p=0.99 and p=0.67, respectively) in S, CT + S and IT + S groups. Furthermore, the results showed a positive correlation between BDNF and H2O2 (r=0.73, p=0.001). Conclusion: Performing of exercise training with maximal sprint can result in greater increase in some factors involved in angiogenesis than continuous training; however vitamin E succinate supplementation has not effect on these angiogenic gains.

کلیدواژه‌ها [English]

  • Continuous training
  • Interval training
  • Vitamin E succinate
  • Vascular endothelial growth factor
  • Hydrogen peroxide

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