تأثیر 8 هفته تمرین مقاومتی برکیناز تنظیم شده توسط پیام خارج سلولی (ERK) تام و فسفریله در عضله تاکننده شست پای موش های صحرایی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 استادیار بخش علوم ورزشی، دانشکده علوم تربیتی و روانشناسی، دانشگاه شیراز، شیراز، ایران.

2 دانشجوی دکتری بیوشیمی و متابولیسم ورزشی، دانشکده علوم تربیتی و روانشناسی، بخش علوم ورزشی، دانشگاه شیراز، شیراز، ایران.

چکیده

زمینه و هدف: تا به امروز بیش از 100 نوع کیناز شناخته شده است، یکی از معروف‌ترین آن­ ها خانواده بزرگ کیناز فعال شده توسط میتوژن (MAPK) است که کیناز تنظیم شده توسط پیام خارج سلولی (ERK) یکی از زیر واحدهای این خانواده می ­باشد؛ ERK بسیاری از عملکردهای مهم سلولی را کنترل می­ کنند اما تأثیر تمرین مقاومتی بر پروتئین ERK به روشنی آشکار نشده است. بنابراین هدف از تحقیق حاضر، بررسی تأثیر 8 هفته تمرین مقاومتی بر ERK تام و فسفریله درعضله تاکننده شست پا (FHL) موش­صحرایی نر سالم بود. روش تحقیق: تعداد 12 سر موش صحرایی نر نژاد اسپراگوداولی به دو گروه مساوی تجربی و کنترل (6=n) تقسیم شدند. گروه تمرین مقاومتی طی 8 هفته و هر هفته 5 جلسه در حالی­که وزنه ­ای به دم آن­ها آویزان بود، از نردبان یک متری بالا می­ رفتند. افزایش بار به صورت هفتگی بر اساس درصدی از وزن بدن به صورت فزآینده اعمال شد. افزایش در هفته اول از 30 درصد شروع و به 200 درصد در هفته هشتم رسید. 48 ساعت پس از آخرین جلسه­ تمرین، عضله FHL استخراج شد و میزان پروتئین­ های مربوطه به روش الایزا اندازه‏ گیری گردیدند. برای تحلیل آماری از روش تحلیل واریانس یک طرفه در سطح معنی داری 0/05>p استفاده شد. یافته­ها: اجرای تمرین مقاومتی موجب افزایش معنی‌دار محتوی پروتئینی تام (0/01=p) شد؛ در حالی که تغییر معنی ‏داری در شکل فسفریله آن ایجاد نگردید (0/08=p). نتیجه­گیری: احتمالا تمرین مقاومتی طولانی مدت مداخله مناسبی جهت فعال­سازی ERK نمی­ باشد. جهت بررسی تغییرات ایجاد شده ناشی از این تمرینات، شاید بهتر باشد مسیرهای پیام ­رسانی دیگر مورد بررسی قرار گیرد.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of 8 weeks of resistance training on total and phosphorylated extracellular signal regulated kinases (ERK) in flexor hallucis longusmuscle of rats

نویسندگان [English]

  • Javad Nemati 1
  • Mahdi Samadi 2
  • Vahid Hadidi 2
  • Leila Ghodrat 2
1 Assistant Professor, Department of Sport Sciences, Faculty of Educational Sciences and Psychology, Shiraz University, Shiraz, Iran
2 PhD Student of Biochemistry for Sport and Exercise Metabolism, Department of Sport Sciences, Faculty of Educational Sciences and Psychology, Shiraz University, Shiraz, Iran
چکیده [English]

Background and Aim: To date, more than 100 types of kinases have been known that one of the most famous of them is the large family of mitochondria -cased kinases (MAPK), which regulated by the out-cell message (ERK). The ERK controls many important cellular functions, but the effect of resistance training on ERK protein has not been clearly revealed. The aim of this study was to investigate the effect of resistance training on the expression of total and phosphorylated ERK proteins in the flexor hallucis longus muscle (FHL) in healthy male rats. Materials and Methods: For this purpose, 12 male Sprague Dawley rats were randomly divided into groups as experimental (n=6) and control (n=6). The experimental groups exerted resistance training including climbing on a ladder during the 8 weeks, 5 sessions per week with a weight hanging on to the tail carried out increased load has been done weekly based on body weight of mice so that the first week was from 30% to 200% in 8 weeks. Fourty eight hours after the last training session, FHL muscle was extracted and the expression of the relevant protein was measured by ELISA. For statistical analysis, one-way ANOVA method was used with a significance level of 0.05. Results: The results showed that resistance exercise significantly increased the total protein content (p=0.01) but had no significantly increased its phosphorylated form (p=0.08). Conclusion: Probably, long-term resistance training is not an appropriate intervention to ERK activation. In order to investigate this exercise induced changes, perhaps it is better to examine other signalling pathways.

کلیدواژه‌ها [English]

  • Extracellular signal-regulated kinase protein
  • resistance training
  • Cell signaling

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