تاثیر 8 هفته تمرین استقامتی و مقاومتی همراه با مصرف عصاره سیر بر بیان ژن پروتئین Bax و مقاومت به انسولین در رت های مبتلا به سندرم متابولیک

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دکتری فیزیولوژی ورزش، دانشکده علوم ورزشی، دانشگاه رازی، کرمانشاه، ایران

2 دانشیار گروه علوم زیستی، دانشکده علوم ورزشی، دانشگاه رازی، کرمانشاه، ایران

3 استاد فیزیولوژی پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکی تبریز، تبریز، ایران

چکیده

زمینه و هدف: بی­تحرکی و تغذیه­ نامتعادل خطر ابتلا به سندرم متابولیک و به دنبال آن بیماری­ های قلبی- عروقی را افزایش  می­دهد. با توجه به اثر تمرین و عصاره سیر بر گلوکز، انسولین و نیمرخ لیپیدی؛ تحقیق حاضر به منظور مقایسه­ اثر 8 هفته­ تمرین استقامتی، مقاومتی و مصرف عصاره­ سیر بر بیان ژن پروتئین Bax، مقاومت به انسولین و نیتریک ­اکساید در رت­های مبتلا به سندرم متابولیک انجام شد. روش تحقیق: چهل و هشت سر رت مبتلا به سندرم متابولیک (12 هفته­ای با میانگین وزنی 25/22±325/36 گرم) در 6 گروه تصادفی همگن شده‌ 8 ­تایی شامل گروه کنترل، تمرین استقامتی، تمرین مقاومتی، تمرین استقامتی + عصاره سیر، تمرین مقاومتی + عصاره سیر و عصاره سیر تقسیم شدند. پس از آشناسازی، رت­های گروه­های آزمایشی در یک پروتکل 8 هفته ای تمرینات استقامتی و مقاومتی (با تکرار 3 جلسه در هفته)  همراه با مصرف عصاره­  سیر (500 میلی­گرم به ازای هرکیلوگرم وزن بدن) شرکت داده شدند. برای بررسی بیان ژن پروتئین Bax و سطوح سرمی متغیرها از روش­های RT-PCR و الایزا استفاده شد. داده­ها با استفاده از آزمون‌های تحلیل واریانس یک سویه و آزمون توکی در سطح 0/05>p بررسی شدند. یافته‌ها: تمرین استقامتی و مقاومتی همراه و بدون مصرف عصاره سیر سبب کاهش معنی­دار بیان ژن پروتئین (Bax (p=0/01 و مقاومت به انسولین  (0/01=p) و افزایش معنی­دار نیتریک ­اکساید (0/01=p)  در رت­ ها شد. به علاوه، دامنه­ افزایش نیتریک ­اکساید در گروه عصاره سیر نسبت به گروه­­های تمرین مقاومتی+ عصاره سیر، تمرین استقامتی و تمرین مقاومتی؛ به طور معنی ­دار (0/0001=p)  بیشتر بود. نتیجه‌گیری: اجرای تمرین استقامتی و تمرین مقاومتی توام با مصرف عصاره سیر، تاثیر بهتری بر تغییرات نامطلوب نیتریک­ اکساید، انسولین و گلوکز در رت­ ها دارد و مانع از بیان شاخص آپوپتوزیس قلبی (ژن Bax) می ­شود.  

کلیدواژه‌ها


عنوان مقاله [English]

Effects of 8 weeks of endurance and resistance training with garlic extract consumption on Bax protein gene expression and insulin resistance in rats with metabolic syndrome

نویسندگان [English]

  • Alireza Rostami 1
  • Vahid Tadibi 2
  • Naser Behpoor 2
  • Naser Ahmadiasl 3
1 Ph.D in Exercise Physiology, Faculty of Sport Sciences, Razi University, Kermanshah, Iran
2 Associate Professor of Biological Sciences Department, Faculty of Sport Sciences, Razi University, Kermanshah, Iran
3 Full Professor in Medical Physiology, Department of Medical, Tabriz University of Medical Sciences,Tabriz, Iran
چکیده [English]

Background and Aim: Inactivity and imbalance diet increase the risk of metabolic syndrome, diabetes, and cardiovascular diseases. Regarding the effects of training and garlic extract consumption on glucose, insulin, and lipid profile; this study was conducted to investigate the effects of 8-weeks of endurance and resistance training, and garlic extract supplementation on Bax gene expression, insulin resistance, and nitric oxide (NO) in rats with metabolic syndrome. Materials and Methods: A total of 48 rats with metabolic syndrome (aged 12-wk, mean weight of 325.36±25.22 g) were randomly divided into 6 groups (N=6) as: endurance training (ET), resistance training (RT), garlic extract supplementation (GE), endurance training along with garlic extract supplementation (ET+GE), resistance training along with garlic extract supplementation (RT+GE), and control (con). After familiarization, rats in the experimental groups completed an 8-weeks (3 sessions a week) ET or RT exercise protocol with or without GE (500 mg.kg-1.day-1) supplementation. The Rt-PCR and the Elisa methods were used to assess the Bax gene expression and the serum levels variables, respectively. Data were analysed using one-way ANOVA and the Tukey test (p˂0.05). Results: The results showed that ET and RT with and without the GE consumption significantly reduced the Bax Gene expression (p=0.01) and the insulin resistance (p=0.01) but for the nitric oxide levels (p=0.01) it showed significantly increased. Moreover, increase of the nitric oxide levels in the GE group was significantly greater than the RT+GE, ET and RT groups (p=0.0001). Conclusion: Endurance and resistance training with GE consumption have superior effects on the nitric oxide, insulin, and glucose in rats and can decrease the expression of cardiac apoptosis marker (Bax gene).

کلیدواژه‌ها [English]

  • Endurance training
  • resistance training
  • Garlic extract
  • Metabolic syndrome

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