The Effect of Different Exercise Training Methods on TIF2 Gene Expression in Subcutaneous Adipose Tissue of Male Wistar Rats

Articles in Press

Document Type : Original Article

Authors

1 Nothing

2 Department of Exercise Physiology, Sport Sciences Institute, Tehran, Iran

3 Department of Sport Sciences, Payam Noor University, Tehran, Iran

10.22077/jpsbs.2025.6780.1961

Abstract

Background and aim: TIF2 is one of the PPAR-γ regulatory cofactors in adipose tissue that can affect lipolysis and thermogenesis processes; however, the effect of different exercise training methods on the expression of this gene in subcutaneous adipose tissue has not been precisely determined. This study aimed to investigate the impact of different training methods on the expression of the TIF2 gene in subcutaneous adipose tissue of male Wistar rats. Materials and Methods: For this purpose, 32 eight-week-old male Wistar rats with an average weight of 237±33 gr were purchased from Razi Institute. The rats were housed in special polycarbonate cages under standard conditions (temperature 22±1.4°C, humidity 55±4%, and a 12:12 h light-dark cycle). During the study, all animals had free access to food and water. After two weeks of adaptation, they were randomly assigned to four groups (N=8):1.Control, 2. Moderate-intensity aerobic training(MIT), 3.High-intensity aerobic training(HIT), and 4. High-intensity interval training(HIIT). The training program was implemented for eight weeks, with five sessions per week. After the end of the training period, the expression level of the TIF2 gene was measured using the RT-PCR method in subcutaneous fat samples of the abdominal area. One-way analysis of variance with LSD test was used at a significant level (P<0.05). Results: The results of the study showed that all three training models significantly reduced TIF2 gene expression in the subcutaneous adipose tissue of rats (P<0.05). However, TIF2 gene expression in the HIT group decreased more than in the HIIT and MIT groups (P=0.001). No significant difference was observed between the training groups (P>0.05). Conclusion: The HIT training model appears to have a greater effect on TIF2 gene expression in subcutaneous adipose tissue than MIT and HIIT, and may also have an effect on other metabolic processes in subcutaneous fat, although further research is needed.

Keywords

Journal of Practical Studies of Biosciences in Sport

Articles in Press, Accepted Manuscript
Available Online from 29 September 2025

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