The effect of aerobic training and MitoQ supplementation on the gene expression of OPA1 and FIS1 in myocardial mitochondrial dynamic pathway in old male rats

Articles in Press

Document Type : Original Article

Authors

1 PhD. Student at Department of Sport Physiology, CT. C., Islamic Azad University, Tehran, Iran

2 Assistant professor at Department of Physical Education and sport Science, Dez. C., Islamic Azad University, Dezful, Iran.

3 Assistant professor at Department of Physical Education and Sport Sciences, Khod.C., Islamic Azad University, Khodabandeh, Iran.

10.22077/jpsbs.2025.8519.1931

Abstract

Background and Aim: One of the problems in most countries of the world is the aging of the population and its consequences are in the occurrence of cardiac diseases. The aim of this study is to investigate the effect of endurance training and MitoQ supplementation on the mitochondrial dynamic signaling pathway (OPA1 and FIS1) in the heart tissue of old male rats. Materials and Methods: In this experimental study, 28 old male Wistar rats were randomly divided into 4 groups of seven rats: control, endurance training, MitoQ supplement and endurance training + MitoQ supplement. After acquainting the animals with the environment for 8 weeks, they performed endurance training for eight weeks (5 days a week) with 70% Vmax. Also, MitoQ supplementation was done at the rate of 250 μM in drinking water for 8 weeks. Real-time PCR method was used to evaluate the variables. The statistical analysis of the data was performed using two-way ANOVA test with a significance level of less than 0.05. Results: Endurance training significantly reduced FIS1 gene expression in myocardial tissue (P = 0.006). MitoQ increased the expression of OPA1 gene in the heart tissue of old rats (P = 0.04), but it has no effect on the FIS1 gene expression (p = 0.006), In addition, the combination of MitoQ and exercise training significantly decreased the gene expression of FIS1 and OPA1 compared to the MitoQ group (P=0.02). Conclusion: MitoQ supplement as an advanced antioxidant can probably play a positive role in the mitochondrial dynamics of the aged cardiac muscle and affect the gene expression of the fusion process. However, the combination of endurance training and MitoQ can modulate the process of mitochondrial fusion and fission.

Keywords

Journal of Practical Studies of Biosciences in Sport

Articles in Press, Accepted Manuscript
Available Online from 08 April 2025

Files

Share

How to cite

Statistics