Document Type : Original Article
Authors
1 MS in Exercise Physiology, Dept. of Sport Sciences, Faculty of Human Sciences, Lorestan University, Khorramabad, Iran.
2 Associate Professor at Dept. of Sport Sciences, Faculty of Human Sciences, Lorestan University, Khorramabad, Iran,
3 Professor at Dept. of Sport Sciences, Faculty of Human Sciences, Lorestan University, Khorramabad, Iran
4 Ph.D. Student in Exercise Physiology, Dept. of Sport Sciences, Faculty of Human Sciences, Lorestan University, Khorramabad, Iran,
Abstract
Background and purpose: The aim of the present study is to investigate the effect of six weeks of progressive aerobic exercise on angiogenic and inflammatory factors of the heart of diabetic male Wistar rats.
Materials and methods: 40 male Wistar rats were randomly divided into four groups: control, diabetic, exercise, and diabetes + exercise. Induction of diabetes was done through intraperitoneal injection of streptozotocin solution. The training protocol consisted of six weeks of progressive aerobic training, five sessions per week on the treadmill. 48 hours after the end of the exercise, all the samples were unconscious and then, following the ethical principles, heart isolation was performed. The gene expression of vascular endothelium growth factor vegf) and tumor necrosis factor-alpha tnf-α) in left ventricular tissue was measured by Real-Time PCR method. One-way analysis of variance, Tukey's post hoc test and Graph Pad version 8 software were used for statistical analysis and the level of statistical significance was considered as P≤0.05.
Findings : The results showed that the expression of the vegf gene in the left ventricle of the heart in the diabetic group was significantly increased compared to the healthy group (p≤0.0001), and the expression of the tnfα gene in the heart tissue was significantly decreased in the diabetic group compared to the healthy group. The expression of vegf gene in the diabetes+exercise group increased significantly compared to the diabetic group (p=0.0036) and the expression of tnfα gene decreased significantly in the diabetes+exercise group compared to the diabetic group.
Conclusion: Six weeks of progressive aerobic training can possibly have an effect on reducing inflammation and increasing vascularity in diabetic samples as a suitable non-pharmacological method, and this effect is carried out by modulating the expression of vegf and tnf-α genes.
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